Degradation of Pentachlorophenol by Potato Polyphenol Oxidase

Hou, Meifang; Tang, Xiaoyan; Zhang, Wei-De; Liao, Lin; Wan, Hongfu

doi:10.1021/jf202236c

Cited by 20 publications

(11 citation statements)

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“…The total reaction mixture volume (2 mL) containing 1 mL of 0.2 M phosphate buffer (pH 7.0), 0.5 mL of culture supernatant and 0.5 mL of 10 mM catechol in 0.2 M phosphate buffer at pH 7.0 was incubated at 50 °C for 3 min and the change in absorbance at 420 nm was measured spectrophotometrically. One unit of enzyme (PPO) activity was defined as the amount of enzyme that increased absorbance of 0.001 per minute (Bevilaqua et al 2002; Hou et al 2011). …”

Section: Methodsmentioning

confidence: 99%

“…Hence, the enzymatic process has an advantage than microorganisms by overcoming these problems for the detoxification of toxic pollutants and is useful for industrial applications. There are reports on copper-containing enzymes like polyphenol oxidases (PPO) which catalyze the oxidation of phenol derivatives in the presence of O 2 (Burton 1994; Rapeanu et al 2006) and also used for the detoxification of other organic contaminants (Bollag et al 2003; Hou et al 2011; Rodriguez Couto and Toca Herrera 2006). However, PPOs from different sources might differ in substrate specificity and catalytic competency (Durán et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of p-cresol degradation with polyphenol oxidase (PPO) immobilized in various matrices

et al. 2016

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p-Cresol is an environmental pollutant due to its vast use, toxicity and persistence, nevertheless, its degradation in an enzyme is unclear. In this study, we used Pleurotus sp. isolate VLECK02 polyphenol oxidase (PPO) for the determination of p-cresol degradation. On the basis of UV, FT-IR and chromatographic (HPLC and GC–MS) analysis, 4-methylcatechol was identified as the main metabolite of p-cresol catabolism. In addition, batch and semi-continuous degradation of p-cresol (10 and 20 mM) were studied and compared by free and immobilized PPO in different matrices like sodium alginate (SA), sodium alginate–polyvinyl alcohol (SA–PVA) and sodium alginate–polyvinyl alcohol–silver nanoparticles (SA–PVA–AgNPs). The experimental data showed that an enzyme (PPO) immobilized in SA–PVA–AgNPs was completely degraded p-cresol at initial concentrations of 10 and 20 mM within 30 h. These results suggest that the enzyme immobilized in SA–PVA–AgNPs has achieved higher degradation rates at a given time than free PPO and PPO immobilized in SA–PVA and SA. The SA–PVA–AgNPs and SA–PVA immobilized enzyme could be reused for more than 12 and 8 cycles, respectively, without losing any degradation capacity. Moreover, the immobilized PPO showed higher tolerance to various temperatures and pH than free PPO. Hence, immobilized PPO could be useful for the bioremediation of environment contaminated with phenolic compounds like p-cresol.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0547-y) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of p-cresol degradation with polyphenol oxidase (PPO) immobilized in various matrices

et al. 2016

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“…PPO has been used to synthesize value-added products such as 3,4-dihydroxy-ʟ- phenylalanine (ʟ-DOPA) for treatment of Parkinson’s disease [6]. PPO from potato or fungi has been used to degrade organic contaminants [7].…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of polyphenol oxidase from fresh ginseng

Kim¹,

Kim²

2013

Journal of Ginseng Research

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Polyphenol oxidase (PPO) was purified from fresh ginseng roots using acetone precipitation, carboxymethyl (CM)-Sepharose chromatography, and phenyl-Sepharose chromatography. Two isoenzymes (PPO 1 and PPO 2) were separated using an ion-exchange column with CM-Sepharose. PPO 1 was purified up to 13.2-fold with a 22.6% yield. PPO 2 bound to CM-Sepharose, eluted with NaCl, and was purified up to 22.5-fold with a 17.4% yield. PPO 2 was further chromatographed on phenyl-Sepharose. The molecular weight of the purified PPO 2 from fresh ginseng was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was about 40 kDa. The optimum temperature and pH were 20℃ and 7.0, respectively, using catechol as a substrate. Pyrogallol showed the highest substrate specificity. The effect of a PPO inhibitor showed that its activity increased slightly in the presence of a low concentration of citric acid. High concentrations of acidic compounds and sulfite agents significantly inhibited purified ginseng PPO 2.

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“…3 The United States Environment Protection Agency (USEPA) considers PCP as a probable carcinogen with severe health hazards. 4 In recent years, the dechlorination of PCP has gained signicant attention, and various technologies have been developed to treat PCP, including zero-valent metal based degradation, 5 biological methods like biosorption and bioremediation, 6 enzyme-catalyzed oxidation, 7,8 photocatalytic degradation, 9 and electrochemical processes. 10 The sorption methods have the disadvantage of only transferring PCP from waste to adsorbent without its actual treatment.…”

Section: Introductionmentioning

confidence: 99%