Degradation of orange dyes and carbamazepine by soybean peroxidase immobilized on silica monoliths and titanium dioxide

Calza, Paola; Zacchigna, Dario; Laurenti, Enzo

doi:10.1007/s11356-016-7399-1

Cited by 43 publications

(36 citation statements)

References 48 publications

(53 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the extraction of soybean peroxidase (SBP) (Tolardo et al, 2019 ), the seeds were peeled, and the obtained hulls were stored at −12°C until use. SBP was extracted and purified by a process based on a previously published method (Calza et al, 2016 ): 100 g of soybean hulls were ground in a mortar, added to 600 mL of phosphate buffer (0.025 M, pH 7) and left under stirring for 2 h at room temperature. Then, the hulls were separated from the solution by filtration with a cotton gauze and subjected to the same treatment until the filtrate gave a negative response to enzymatic activity test for SBP.…”

Section: Methodsmentioning

confidence: 99%

A Way to Close the Loop: Physicochemical and Adsorbing Properties of Soybean Hulls Recovered After Soybean Peroxidase Extraction

et al. 2020

Self Cite

View full text Add to dashboard Cite

Soybean hulls are one of the by-products of soybean crushing and find application mainly in the animal feed sector. Nevertheless, soybean hulls have been already exploited as source of peroxidase (soybean peroxidase, SBP), an enzyme adopted in a wide range of applications such as bioremediation and wastewater treatment, biocatalysis, diagnostic tests, therapeutics and biosensors. In this work, the soybean hulls after the SBP extraction, destined to become a putrescible waste, were recovered and employed as adsorbents for water remediation due to their cellulose-based composition. They were studied from a physicochemical point of view using different characterization techniques and applied for the adsorption of five inorganic ions [Fe(III), Al(III), Cr(III), Ni(II), and Mn(II)] in different aqueous matrixes. The behavior of the exhausted soybean hulls was compared to pristine hulls, demonstrating better performances as pollutant adsorbents despite significant changes in their features, especially in terms of surface morphology, charge and composition. Overall, this work evidences that these kinds of double-recovered scraps are an effective and sustainable alternative for metal contaminants removal from water.

show abstract

Section: Methodsmentioning

confidence: 99%

A Way to Close the Loop: Physicochemical and Adsorbing Properties of Soybean Hulls Recovered After Soybean Peroxidase Extraction

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the last decades, new chemicals were detected in wastewaters as a result of new industrial processes and increased consumption of pharmaceuticals and personal care products [3,4].…”

Section: Introductionmentioning

confidence: 99%

“…Out of methods listed above, the adsorption technique [14] has attracted considerable attention because of the simple procedures needed, because no toxic substances are produced, because of ability to treat concentrated forms of the pollutants, and to reuse the spent adsorbent via regeneration. Moreover, it plays a central role in drinking water purification and wastewater treatments [3,7,15,16].…”

Section: Introductionmentioning

confidence: 99%

An Easy Synthesis for Preparing Bio-Based Hybrid Adsorbent Useful for Fast Adsorption of Polar Pollutants

et al. 2019

Self Cite

View full text Add to dashboard Cite

For the first time, γ-Al2O3 and Bio-Based Substances (BBS) hybrids (A-BBS) were prepared through a simple electrostatic interaction occurring between alumina, used as a support, and BBS (Bio-Based Substance from composted biowastes) carrying positive and negative charges, respectively. We evaluated the optimal amount of BBS to be immobilized on the support and the stability of the resulting A-BBS in order to use this novel hybrid material as an adsorbent for the removal of polar pollutants. Characterization was carried out by X-Ray Diffraction (XRD) for evaluating the crystal structure of the support, Fourier transform infrared spectroscopy (FT-IR) to evidence the presence of BBS on the hybrid material, thermogravimetric analysis (TGA) to measure the thermal stability of the hybrid materials and quantify the BBS amount immobilized on the support, N2 adsorption at 77 K for the evaluation of the surface area and porosity of the systems, Zeta potential measurements to evaluate the effect of BBS immobilization on the surface charge of the particles and choose the substrates possibly interacting with them. Firstly, we tested the adsorption capability of three samples differently coated with BBS toward cationic species considering various adsorbate/adsorbent ratio. Crystal Violet (CV) was chosen as model pollutant to compare the performance of the hybrid materials with those of other materials described in the literature. The adsorption data were modeled by Langmuir and Freundlich adsorption isotherms. Then, we studied the adsorption capability of the developed material towards molecules with different structures; for this purpose, two contaminants of emerging concerns (carbamazepine and atenolol) were tested. The results indicate that A-BBS could be applied in wastewater treatment for the removal of a significant amount of polar species. In addition, a comparison with literature data concerning CV adsorption was carried out in order to evaluate the environmental impact of synthetic routes used to prepare different adsorbents.

show abstract

“…Many authors showed the effectiveness of SBP in the oxidation of chlorophenols [14][15][16][17][18]. The ability of SBP in the degradation of 2,4,6-trichlorophenol (TCP) was previously studied by Calza et al [19] using composite material under UV irradiation.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Degradation of 2,4,6-Trichlorophenol in a Microreactor using Soybean Peroxidase

et al. 2020

Self Cite

View full text Add to dashboard Cite

Soybean peroxidase is an enzyme extracted from soybean seed hulls. In the presence of hydrogen peroxide, the enzyme has the potential to catalyze the biodegradation of toxic substances like chlorophenols. For this reason, its use in wastewater treatment processes is environmentally friendly since the enzyme can be obtained from a renewable and abundant raw material. In this work, enzymatic biodegradation of 2,4,6-trichlorophenol performed by soybean peroxidase in a microreactor was studied experimentally and theoretically. The experimental data set was obtained with a volume of 250 μL by using different soybean peroxidase concentrations and different reaction times. The fluid dynamics of the microreactor was modeled as well, using ANSYS CFX. The simulations exhibited secondary flows, which enhanced mixing. Although the laminar flow was developed, it can be assumed to be a well-mixed medium. The kinetic data were evaluated through a mechanistic model, the modified bi-bi ping-pong model, which is adequate to represent the enzymatic degradation using peroxidases. The model was composed of an initial value problem for ordinary differential equations that were solved using MATLAB. Some kinetic constants were estimated using the least square function. The results of the model fit well the experimental data.

show abstract

Degradation of orange dyes and carbamazepine by soybean peroxidase immobilized on silica monoliths and titanium dioxide

Cited by 43 publications

References 48 publications

A Way to Close the Loop: Physicochemical and Adsorbing Properties of Soybean Hulls Recovered After Soybean Peroxidase Extraction

A Way to Close the Loop: Physicochemical and Adsorbing Properties of Soybean Hulls Recovered After Soybean Peroxidase Extraction

An Easy Synthesis for Preparing Bio-Based Hybrid Adsorbent Useful for Fast Adsorption of Polar Pollutants

Enzymatic Degradation of 2,4,6-Trichlorophenol in a Microreactor using Soybean Peroxidase

Contact Info

Product

Resources

About