Soybean peroxidase is an enzyme extracted from soybean seed hulls. In the presence of hydrogen peroxide, the enzyme has the potential to catalyze the biodegradation of toxic substances like chlorophenols. For this reason, its use in wastewater treatment processes is environmentally friendly since the enzyme can be obtained from a renewable and abundant raw material. In this work, enzymatic biodegradation of 2,4,6-trichlorophenol performed by soybean peroxidase in a microreactor was studied experimentally and theoretically. The experimental data set was obtained with a volume of 250 μL by using different soybean peroxidase concentrations and different reaction times. The fluid dynamics of the microreactor was modeled as well, using ANSYS CFX. The simulations exhibited secondary flows, which enhanced mixing. Although the laminar flow was developed, it can be assumed to be a well-mixed medium. The kinetic data were evaluated through a mechanistic model, the modified bi-bi ping-pong model, which is adequate to represent the enzymatic degradation using peroxidases. The model was composed of an initial value problem for ordinary differential equations that were solved using MATLAB. Some kinetic constants were estimated using the least square function. The results of the model fit well the experimental data.
Robust data reconciliation (RDR) is an effective technique designed to minimize/annul gross errors drawbacks over estimated process variables. In the present work, a brief review on heuristic optimization methods devoted to RDR in chemical processes is performed. Twelve robust estimators were evaluated, including Smith and Bell estimators, which were never before used within this context. The performance of these estimators was evaluated in a Van de Vusse reaction system, described by a nonlinear system of equations. The problem was solved by both the simulated annealing method and the IPOPT framework. The results showed the efficiency and consistency of both schemes and, despite the relevance of gross errors, the redescending estimators exhibited better global performance.
In recent years, bioremediation has become very attractive for environmental applications, especially enzyme-based treatments, since they offer high catalytic capacity with milder reaction conditions, production of nontoxic compounds, and environmental...
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