Degradation of dichloroaniline isomers by a newly isolated strain, Bacillus megaterium IMT21

Yao, Xiefeng; Khan, Fazlurrahman; Pandey, Rinku; Pandey, Janmejay; Mourant, Roslyn; Jain, Rakesh; Guo, Jianhua; Russell, Robyn J.; Oakeshott, John G.; Pandey, Gyanendra

doi:10.1099/mic.0.045393-0

Cited by 49 publications

(35 citation statements)

References 26 publications

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“…Similarly, Canton et al [11] also classified 2-C-4-NA as a blacklist substance due to its poor biodegradability. However, based on the previous reports on the biodegradation of structural analogues of 2-C-4-NA such as 4-nitroaniline, 2-, 3-, 4-chloroaniline and 3, 4-dichloroaniline, possibilities for the biodegradation of 2-C-4-NA by microorganisms could also be presumed [12]–[18]. In this communication, we report metabolic characterization of 2-C-4-NA by Rhodococcus sp.…”

Section: Introductionmentioning

confidence: 86%

Metabolism of 2-Chloro-4-Nitroaniline via Novel Aerobic Degradation Pathway by Rhodococcus sp. Strain MB-P1

et al. 2013

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2-chloro-4-nitroaniline (2-C-4-NA) is used as an intermediate in the manufacture of dyes, pharmaceuticals, corrosion inhibitor and also used in the synthesis of niclosamide, a molluscicide. It is marked as a black-listed substance due to its poor biodegradability. We report biodegradation of 2-C-4-NA and its pathway characterization by Rhodococcus sp. strain MB-P1 under aerobic conditions. The strain MB-P1 utilizes 2-C-4-NA as the sole carbon, nitrogen, and energy source. In the growth medium, the degradation of 2-C-4-NA occurs with the release of nitrite ions, chloride ions, and ammonia. During the resting cell studies, the 2-C-4-NA-induced cells of strain MB-P1 transformed 2-C-4-NA stoichiometrically to 4-amino-3-chlorophenol (4-A-3-CP), which subsequently gets transformed to 6-chlorohydroxyquinol (6-CHQ) metabolite. Enzyme assays by cell-free lysates prepared from 2-C-4-NA-induced MB-P1 cells, demonstrated that the first enzyme in the 2-C-4-NA degradation pathway is a flavin-dependent monooxygenase that catalyzes the stoichiometric removal of nitro group and production of 4-A-3-CP. Oxygen uptake studies on 4-A-3-CP and related anilines by 2-C-4-NA-induced MB-P1 cells demonstrated the involvement of aniline dioxygenase in the second step of 2-C-4-NA degradation. This is the first report showing 2-C-4-NA degradation and elucidation of corresponding metabolic pathway by an aerobic bacterium.

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Section: Introductionmentioning

confidence: 86%

Metabolism of 2-Chloro-4-Nitroaniline via Novel Aerobic Degradation Pathway by Rhodococcus sp. Strain MB-P1

et al. 2013

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“…B. megaterium has earlier been reported for its potential against various pollutants such as PAH (polycyclic aromatic hydrocarbons), dyes and chromium. [36,37] Its bifunctional Cytochrome P450 enzyme has been highlighted by various catabolic studies. [38] Thus, strains KKc3, KKc4 and KKc6 detoxified endosulfan, whereas KKc7 followed the toxic endosulfan sulphate pathway.…”

Section: Resultsmentioning

confidence: 99%

Chemotaxis-based endosulfan biotransformation: enrichment and isolation of endosulfan-degrading bacteria

Seralathan

Sivanesan

Nargunanathan

et al. 2014

Environmental Technology

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The study was conducted to isolate endosulfan biotransforming or biodegrading microbes based on chemotaxis. Pseudomonas aeruginosa strain KKc3, Ochrobactrum sp. strain KKc4, Achromobacter xylosoxidans strain KKc6 and Bacillus megaterium KKc7 were isolated based on their migration towards endosulfan in a soil column. Out of the four bacteria, B. megaterium converted endosulfan into toxic metabolite endosulfan sulphate, while the other three bacteria followed the non-toxic endosulfan diol pathway. The mixed culture system consisting of P. aeruginosa, Ochrobactrum sp and A. xylosoxidans could remove 94% of total endosulfan by using endosulfan as the sole source of sulphur.

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“…Bacteria often possess the ability to degrade xenobiotic compounds (Vamsee-Krishna et al, 2006;Cycon et al, 2009). The degradation of propanil by the rice rhizosphere bacteria shows that these organisms had adapted to metabolize this chemical after repeated rounds of exposure in the field (Sarkar et al, 2010;Yao et al, 2011). Earlier studies have suggested that pesticides applied to soil are often degraded more rapidly following repeated application to the same site, where the soil then possesses bacteria capable of degrading the contaminants (Witzig et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Diversity of propanil-degrading bacteria isolated from rice rhizosphere and their potential for plant growth promotion

Procópio¹,

Procopio²,

Pizzirani-Kleiner³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. The herbicide propanil has long been used in rice production in southern Brazil. Bacteria isolated from contaminated soils in Massaranduba, Santa Catarina, Brazil, were found to be able to grow in the presence of propanil, using this compound as a carbon source. Thirty strains were identified as Pseudomonas (86.7%), Serratia (10.0%), and Acinetobacter (3.3%), based on phylogenetic analysis of 16S rDNA. Little genetic diversity was found within species, more than 95% homology, suggesting that there is selective pressure to metabolize propanil in the microbial community. Two strains of Pseudomonas (AF7 and AF1) were selected in bioreactor containing chemotactic growth medium, with the highest degradation activity of propanil exhibited by strain AF7, followed by AF1 (60 and 40%, respectively). These strains when encapsulated in alginate exhibited a high survival rate and were able to colonize the rice root surfaces. Inoculation with Pseudomonas strains AF7 and AF1 significantly improved the plant height of rice. Most of the Pseudomonas strains produced indoleacetic acid, soluble mineral phosphate, and fixed nitrogen. These bacterial strains could potentially be used for the bioremediation of propanil-contaminated soils and the promotion of plant growth.

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Degradation of dichloroaniline isomers by a newly isolated strain, Bacillus megaterium IMT21

Cited by 49 publications

References 26 publications

Metabolism of 2-Chloro-4-Nitroaniline via Novel Aerobic Degradation Pathway by Rhodococcus sp. Strain MB-P1

Metabolism of 2-Chloro-4-Nitroaniline via Novel Aerobic Degradation Pathway by Rhodococcus sp. Strain MB-P1

Chemotaxis-based endosulfan biotransformation: enrichment and isolation of endosulfan-degrading bacteria

Diversity of propanil-degrading bacteria isolated from rice rhizosphere and their potential for plant growth promotion

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