Chemotaxis-based endosulfan biotransformation: enrichment and isolation of endosulfan-degrading bacteria

Seralathan, Muhil Vannan; Sivanesan, Saravanadevi; Nargunanathan, Srinivasan; Bafana, Amit; Krishnamurthi, Kannan; Chakrabarti, Tapan

doi:10.1080/09593330.2014.937464

Cited by 11 publications

(3 citation statements)

References 41 publications

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“…In addition, plasmid conjugation machinery has been identified in Ochrobactrum plasmids ( 20 , 42 ), and horizontal gene transfer by conjugation was demonstrated in this genus ( 47 , 48 ). Ochrobactrum species was previously found to degrade a wide spectrum of recalcitrant and xenobiotic compounds, such as organophosphates ( 49 ), tetrachloroethene ( 50 ), dichloro-diphenyl-trichloroethane (DDT) ( 51 ), 2,4-dichlorophenoxyacetic acid, endosulfan ( 52 ) and aniline ( 53 ), among many others. Interestingly, ligninolytic activity was also reported from Ochrobactrum species ( 39 , 41 ).…”

Section: Resultsmentioning

confidence: 99%

Identification and Characterization of a Novel Plasmid-Encoded Laccase-Like Multicopper Oxidase from Ochrobactrum sp. BF15 Isolated from an On-Farm Bio-Purification System

Martini

Berini

Ausec

et al. 2021

Food Technol. Biotechnol. (Online)

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Research background. In recent decades, laccases (p-diphenol-dioxygen oxidoreductases; EC 1.10.3.2) have attracted the attention of researchers due to their wide range of biotechnological and industrial applications. Laccases can oxidize a variety of organic and inorganic compounds, making them suitable as biocatalysts in biotechnological processes. Even though the most traditionally used laccases in the industry are of fungal origin, bacterial laccases have shown an enormous potential given their ability to act on several substrates and in multiple conditions. The present study aims to characterize a plasmid-encoded laccase-like multicopper oxidase (LMCO) from Ochrobactrum sp. BF15, a bacterial strain previously isolated from polluted soil. Experimental approach. We used in silico profiles Hidden Markov Models to identify novel laccase-like genes in Ochrobactrum sp. BF15. For laccase characterization, we performed heterologous expression in E. coli, purification and activity measurement on typical laccase substrates. Results and conclusions. Profiles Hidden Markov Models allowed us to identify a novel LMCO, named Lac80. In silico analysis of Lac80 revealed the presence of the three conserved copper-oxidase domains characteristic of three-domain laccases. We successfully expressed Lac80 heterologously in Escherichia coli, allowing us to purify the protein for further activity evaluation. Of thirteen typical laccase substrates tested, Lac80 showed discrete activity on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), pyrocatechol, pyrogallol, and vanillic acid, and higher activity on 2,6-dimethoxyphenol. Novelty and scientific contribution. Our results point out Lac80 as a promising laccase for use in industrial applications. The present work shows the relevance of bacterial laccases and highlights the importance of environmental plasmids as valuable sources of new genes encoding enzymes with potential use in biotechnological processes.

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Section: Resultsmentioning

confidence: 99%

Identification and Characterization of a Novel Plasmid-Encoded Laccase-Like Multicopper Oxidase from Ochrobactrum sp. BF15 Isolated from an On-Farm Bio-Purification System

Martini

Berini

Ausec

et al. 2021

Food Technol. Biotechnol. (Online)

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“…For example, Kumar and Pannu [ 83 ] identified Rhodanobacter lindaniclasticus, Alkaligens faecalis , and Pseudomonas aeruginosa as capable of dechlorinating lindane, Wang, et al [ 84 ] reported that Stroptomyces sp. strain can degrade DDT, and Seralathan, et al [ 85 ] confirmed that Pseudomonas aeruginosa, Ochrobacterium sp, and Achromobacter xylosoxidans degrade endosulfan and use it as a source of sulfur. These organochlorine pesticide-degrading species contain genes, e.g., lin and Esd genes that encode for dehalogenases, hydrolases, dehydrochlorinases, and monooxygenases enzymes that take part in mineralizing these compounds [ 86 ].…”

Section: Bioremediation Of Recalcitrant Organic Contaminantsmentioning

confidence: 99%

Composting and its application in bioremediation of organic contaminants

et al. 2022

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This review investigates the findings of the most up-to-date literature on bioremediation via composting technology. Studies on bioremediation via composting began during the 1990s and have exponentially increased over the years. A total of 655 articles have been published since then, with 40% published in the last six years. The robustness, low cost, and easy operation of composting technology make it an attractive bioremediation strategy for organic contaminants prevalent in soils and sediment. Successful pilot-and large-scale bioremediation of organic contaminants, e.g., total petroleum hydrocarbons, plasticizers, and persistent organic pollutants (POPs) by composting, has been documented in the literature. For example, composting could remediate >90% diesel with concentrations as high as 26,315 mg kg −a of initial composting material after 24 days. Composting has unique advantages over traditional single- and multi-strain bioaugmentation approaches, including a diverse microbial community, ease of operation, and the ability to handle higher concentrations. Bioremediation via composting depends on the diverse microbial community; thus, key parameters, including nutrients (C/N ratio = 25–30), moisture (55–65%), and oxygen content (O 2 > 10%) should be optimized for successful bioremediation. This review will provide bioremediation and composting researchers with the most recent finding in the field and stimulate new research ideas.

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“…(Verma et al 2011), Achromobacter xylosoxidans, Bordetella sp. (Sand and Singh 2009), Klebsiella, Acinetobacter, Alcaligenes, Flavobacterium, Bacillus (Kafilzadeh et al 2015), and A. xylosoxidans (Seralathan et al 2015). Diagrammatic representation of endosulfan biodegradation by Pseudomonas mendocina ZAM1 strain is depicted in Fig.…”

Section: Introductionmentioning

confidence: 99%

Degradation and conversion of endosulfan by newly isolated Pseudomonas mendocina ZAM1 strain

Mir¹,

Ali

Tyagi

et al. 2017

3 Biotech

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Endosulfan contamination is one of the major concerns of soil ecosystem, which causes detrimental effects not only to humans but also to animals and plants. Therefore, the aim of this study was to isolate and identify a novel bacterial strain capable of degrading endosulfan in agriculture contaminated soils. A novel bacterial strain was isolated from the sugarcane field contaminated with endosulfan, and was named as ZAM1 strain. The ZAM1 bacterial strain was further identified as Pseudomonas mendocina based on the biochemical and molecular analysis. 16sRNA sequence analysis of ZAM1 strain shows maximum similarity with known endosulfan-degrading bacteria (Pseudomonas putida), respectively. Enrichment was carried out using the endosulfan as sole sulfur source. The ZAM1 strain was able to use α and β endosulfan as a sole sulfur source. Our results showed that ZAM1 strain degrades endosulfan >64.5% (50 mg/l) after 12 days of incubation. The residues were analyzed by GC-MS analysis and confirmed the formation of metabolites of dieldrin, 2 heptanone, methyl propionate, and endosulfan lactone compounds. Hence, these results indicate that the ZAM1 strain is a promising bacterial source for detoxification of endosulfan residues in the environment.

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Chemotaxis-based endosulfan biotransformation: enrichment and isolation of endosulfan-degrading bacteria

Cited by 11 publications

References 41 publications

Identification and Characterization of a Novel Plasmid-Encoded Laccase-Like Multicopper Oxidase from Ochrobactrum sp. BF15 Isolated from an On-Farm Bio-Purification System

Identification and Characterization of a Novel Plasmid-Encoded Laccase-Like Multicopper Oxidase from Ochrobactrum sp. BF15 Isolated from an On-Farm Bio-Purification System

Composting and its application in bioremediation of organic contaminants

Degradation and conversion of endosulfan by newly isolated Pseudomonas mendocina ZAM1 strain

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