Degradation of Chrysanthemum (Dendranthema grandiflora) wastes by Pleurotus ostreatus for the production of reducing sugars

Quevedo-Hidalgo, Balkys; Narváez-Rincón, Paulo César; Pedroza-Rodríguez, Aura M.; Velásquez-Lozano, Mario Enrique

doi:10.1007/s12257-012-0227-7

Cited by 11 publications

(20 citation statements)

References 35 publications

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“…The CBHII activity was carried out as previously described [ 18 , 19 ], using 5 mM p-nitrophenyl β -D-cellobioside (pNPC, Sigma-Aldrich) in sodium acetate buffer 50 mM, pH 5.0. One enzyme unit was defined as the amount of enzyme releasing 1 μ mol of p-nitrophenol per min, and the activity was expressed as U L −1 .…”

Section: Methodsmentioning

confidence: 99%

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Production of Recombinant Trichoderma reesei Cellobiohydrolase II in a New Expression System Based on Wickerhamomyces anomalus

et al. 2017

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Cellulase is a family of at least three groups of enzymes that participate in the sequential hydrolysis of cellulose. Recombinant expression of cellulases might allow reducing their production times and increasing the low proteins concentrations obtained with filamentous fungi. In this study, we describe the production of Trichoderma reesei cellobiohydrolase II (CBHII) in a native strain of Wickerhamomyces anomalus. Recombinant CBHII was expressed in W. anomalus 54-A reaching enzyme activity values of up to 14.5 U L−1. The enzyme extract showed optimum pH and temperature of 5.0–6.0 and 40°C, respectively. Enzyme kinetic parameters (KM of 2.73 mM and Vmax of 23.1 µM min−1) were between the ranges of values reported for other CBHII enzymes. Finally, the results showed that an enzymatic extract of W. anomalus 54-A carrying the recombinant T. reesei CBHII allows production of reducing sugars similar to that of a crude extract from cellulolytic fungi. These results show the first report on the use of W. anomalus as a host to produce recombinant proteins. In addition, recombinant T. reesei CBHII enzyme could potentially be used in the degradation of lignocellulosic residues to produce bioethanol, based on its pH and temperature activity profile.

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Section: Methodsmentioning

confidence: 99%

“…The effect of recombinant CBHII on cellulose hydrolysis was evaluated by using Chrysanthemum wastes, as previously described [ 18 , 19 ], with modifications. Chrysanthemum wastes were autoclaved at 121°C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Production of Recombinant Trichoderma reesei Cellobiohydrolase II in a New Expression System Based on Wickerhamomyces anomalus

et al. 2017

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“…Biological treatment using white rot fungi such as T. versicolor involves multiple biochemical and physical reactions that can be carried out simultaneously, like the breakdown of intermolecular bonds, demethylation, hydroxylation, dechlorination, and the opening of the aromatic ring [4]. All of these transformations are developed together through the combined action of several enzymes, for example, laccase, manganese peroxidase, lignin peroxidase, xylanases, veratryl alcohol oxidase, and so forth [5,6]. Other mechanisms are related to physicochemical interactions such as adsorption, deposition, and ion exchange [7].…”

Section: Introductionmentioning

confidence: 99%

Optimization of C/N Ratio and Inducers for Wastewater Paper Industry Treatment Using Trametes versicolor Immobilized in Bubble Column Reactor

Pedroza-Rodríguez

Rodrı́guez-Vázquez

2013

Journal of Mycology

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C/N ratio and MnSO4and CuSO4concentrations were optimized for decolorization and chemical oxygen demand (COD) removal of bleached Kraft pulp mill effluent byTrametes versicolorimmobilized in polyurethane foam. Statistical differences (P<0.0001) at high C/N ratios (169), 2 mM CuSO4, and 0.071 mM MnSO4were determined. Decolorization of 60.5%, COD removal of 55%, laccase (LAC) 60 U/L, and manganese peroxidase (MnP) 8.4 U/L were obtained. Maximum of decolorization (82%), COD removal (83%), LAC (443.5 U/L), and MnP (18 U/L) activities at C/N ratio of 405 (6.75 mM CuSO4and 0.22 mM MnSO4) was achieved in step 7 at 4 d. Positive correlation between the decolorization, COD removal, and enzymatic activity was found (P<0.0001).T. versicolorbioremediation capacity was evaluated in bubble column reactor during 8 d. Effluent was adjusted according to optimized parameters and treated at 25°C and air flow of 800 mL/min. Heterotrophic bacteria growth was not inhibited by fungus. After 4 d, 82% of COD reduction and 80% decolorization were recorded. Additionally, enzymatic activity of LAC (345 U/L) and MnP (78 U/L) was observed. The COD reduction and decolorization correlated positively (P<0.0001) with enzymatic activity. Chlorophenol removal was 98% of pentachlorophenol (PCP), 92% of 2,4,5-trichlorophenol (2,4,5-TCP), 90% of 3,4-dichlorophenol (3,4-DCP), and 99% of 4-chlorophenols (4CP).

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“…The optimum conditions for Chrysanthemum and Rosa residues were 6.3 % (w/v) concentration and pH 5.6, and 11.8 % (w/v) concentration and pH 5.6, respectively (Quevedo-Hidalgo et al 2012). For the assays without inductors, Cu +2 and Mn +2 concentrations were the same as those originally present in each residue.…”

Section: Enzyme Production From Chrysanthemum and Rosamentioning

confidence: 99%

“…To exploit the potential of lignocellulose as feedstock to produce high added-value products, a physical, chemical or biological pretreatment process is always necessary to modify its three-dimensional structure. Some biological pretreatments have been studied, among these; degradation using enzymes produced by fungi (Stajić et al 2006, Membrillo et al 2008, Bettin et al 2011, Větrovský et al 2013, including the lignocellulosic degradation using P. ostreatus (Isikhuemhen & Mikiashvilli 2009, Quevedo-Hidalgo 2012, Thakur et al 2013.…”

Section: Introductionmentioning

confidence: 99%

Production of lignocellulolytic enzymes from floriculture residues using Pleurotus ostreatus

et al. 2014

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Floriculture is a vital agro-industrial sector in the Colombian economy; the export of flowers positively impacts employment and the balance of trade. However, this industry could negatively impact the environment if its waste products are not handled properly. These flower residues, rich in lignin, hemicellulose and cellulose, could be a cost-effective raw material to produce enzymes. Here, we evaluate the production of lignocellulolytic enzymes by degradation of Chrysanthemum and Rosa residues using Pleurotus ostreatus, and manganese sulfate and copper sulfate as inductors. From the two residues, we obtained laccase, manganese peroxidase, endoglucanase, exoglucanase, and β-glucosidase. The use of inductors, favored all enzyme activities except for β-glucosidase. The enzymes that displayed the highest activity were laccase (4,693.4 U/L and 2,640 U/L from the residues of Chrysanthemum and Rosa, respectively) and β-glucosidase (9,513 U/L and 6,811.9 U/L). The enzyme that showed the lowest activity was endoglucanase (11.5 U/L and 15.4 U/L). Under the conditions evaluated, the best substrate for enzyme production is Chrysanthemum wastes; the extracts obtained had higher enzymatic activity than the extracts from Rosa residues.

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Degradation of Chrysanthemum (Dendranthema grandiflora) wastes by Pleurotus ostreatus for the production of reducing sugars

Cited by 11 publications

References 35 publications

Production of Recombinant Trichoderma reesei Cellobiohydrolase II in a New Expression System Based on Wickerhamomyces anomalus

Production of Recombinant Trichoderma reesei Cellobiohydrolase II in a New Expression System Based on Wickerhamomyces anomalus

Optimization of C/N Ratio and Inducers for Wastewater Paper Industry Treatment Using Trametes versicolor Immobilized in Bubble Column Reactor

Production of lignocellulolytic enzymes from floriculture residues using Pleurotus ostreatus

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