Production of lignocellulolytic enzymes from floriculture residues using Pleurotus ostreatus

Abstract

Floriculture is a vital agro-industrial sector in the Colombian economy; the export of flowers positively impacts employment and the balance of trade. However, this industry could negatively impact the environment if its waste products are not handled properly. These flower residues, rich in lignin, hemicellulose and cellulose, could be a cost-effective raw material to produce enzymes. Here, we evaluate the production of lignocellulolytic enzymes by degradation of Chrysanthemum and Rosa residues using Pleurotus ostreatus, and manganese sulfate and copper sulfate as inductors. From the two residues, we obtained laccase, manganese peroxidase, endoglucanase, exoglucanase, and β-glucosidase. The use of inductors, favored all enzyme activities except for β-glucosidase. The enzymes that displayed the highest activity were laccase (4,693.4 U/L and 2,640 U/L from the residues of Chrysanthemum and Rosa, respectively) and β-glucosidase (9,513 U/L and 6,811.9 U/L). The enzyme that showed the lowest activity was endoglucanase (11.5 U/L and 15.4 U/L). Under the conditions evaluated, the best substrate for enzyme production is Chrysanthemum wastes; the extracts obtained had higher enzymatic activity than the extracts from Rosa residues.

Detoxification of pulping black liquor with Pleurotus ostreatus or recombinant Pichia pastoris followed by CuO/TiO2/visible photocatalysis

“…The removal capacity of P. ostreatus is associated with the native fungus ligninolytic enzyme system. This process involves different laccases, H 2 O 2 -dependent enzymes (manganese peroxidase, and lignin peroxidase), and hydrogen peroxide-generating systems 24 , 38 , 39 . The ligninolytic system can act on BL, white liquor, or lignosulfonates, which are rich in phenolic compounds with various molecular weights, via successive oxidation reactions to form cationic radicals, aliphatic compounds, and possible mineralization leading to CO 2 production 40 .…”

Partial removal and detoxification of Malachite Green and Crystal Violet from laboratory artificially contaminated water by Pleurotus ostreatus

“…Laccase activity was determined following the protocol reported by Tinoco et al, 2001. Manganese peroxidase (MnP) activity was determined following the protocol reported by Quevedo-Hidalgo et al, 2015. …”

Production of Pleurotus sajor-caju crude enzyme broth and its applicability for the removal of bisphenol A

“…Fungi of the Pleurotus genus are considered to be good producers of enzymes such as cellulases, hemicellulases, laccases, manganese peroxidases and lignin peroxidases (Libardi-Junior et al 2011, 2012, Pandey et al 2017. They grow in a wide variety of agro-industrial wastes and are easily cultivated in both liquid and solid media (Bonatti et al 2004, Elisashvili et al 2008, Quevedo-Hidalgo et al 2015. The northeastern region of Santa Catarina, Brazil, is a large banana producer and, consequently, a large generator of agro-industrial wastes which can be used in biotechnological routes, such as fungal enzyme production, reducing production costs and heling in solving environmental problems arising from their accumulation in nature (Bonatti et al 2004, Osma et al 2011, Pandey et al 2017.…”

“…The northeastern region of Santa Catarina, Brazil, is a large banana producer and, consequently, a large generator of agro-industrial wastes which can be used in biotechnological routes, such as fungal enzyme production, reducing production costs and heling in solving environmental problems arising from their accumulation in nature (Bonatti et al 2004, Osma et al 2011, Pandey et al 2017. These wastes are defined as carbon sources in the composition of the culture medium, but, at the same time, also as inductors for the production of lignocellulolytic enzymes by fungi (Elisashvili et al 2008, Rashad et al 2010, Libardi-Junior et al 2012, Tinoco-Valência et al 2014, Quevedo-Hidalgo et al 2015.…”