Degradation of carbondisulphide by a Thiobacillus isolate

Plas, Christian; Wimmer, Katharina; Holubar, P.; Mattanovich, Diethard; Danner, H; Jelinek, Eveline; Harant, Hanna; Braun, Rudolf

doi:10.1007/bf00167151

Cited by 23 publications

(11 citation statements)

References 18 publications

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“…The kinetics of the oxidation of CS 2 by the strain TJ 330 differ from the parameters reported earlier for bacterial CS 2 oxidation. Smith and Kelly (1988) and Plas et al . (1993) reported K s values of 16·5 µmol l −1 for T. thioparus strain TK‐m, and 25 µmol l −1 for another Thiobacillus isolate.…”

Section: Discussionmentioning

confidence: 93%

“…This strain produced carbonyl sulphide (COS) and hydrogen sulphide (H 2 S) anaerobically, and these were also assumed to be the first intermediates in aerobic metabolism. Strain TK‐m was active in the presence of CS 2 in a neutral environment, as was the Thiobacillus strain studied by Plas et al . (1993) and four eubacterial strains isolated from the soil and leaves of a CS 2 ‐producing tree ( Jordan et al .…”

Section: Introductionmentioning

confidence: 79%

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Physiology and taxonomy of Thiobacillus strain TJ330, which oxidizes carbon disulphide (CS2)

Hartikainen¹,

Ruuskanen²,

Räty

et al. 2000

J Appl Microbiol

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A bacterium (strain TJ330) capable of using carbon disulphide (CS 2 ) as its sole energy source in an acidic environment was isolated from a peat bio®lter used in experiments to remove CS 2 and hydrogen sulphide (H 2 S) from air. Its physiology and taxonomy are described here. The strain oxidized CS 2 , H 2 S and elemental sulphur to sulphate chemolithotrophically. The rate of sulphate production was highest at pH 2. The maximum growth rate constant (m max ) using CS 2 as a substrate was 3Á9 Â 10 À2 h À1 (generation time 18 h) and the Monod constant (K s ) was 0Á97±2Á6 mmol l À1 CS 2 (74±198 mg l À1 ), corresponding to an equilibrium with 15±40 ppm CS 2 in the headspace. The optimum growth temperature using elemental sulphur as a substrate was 28 C. The strain bears morphological and physiological similarities to Thiobacillus thiooxidans, but the latter is incapable of oxidizing CS 2 . The strain TJ330 (DSM 8985) showed only 44Á2 11Á8% DNA homology with the type strain T. thiooxidans ATCC 19377, while its homology with T. ferrooxidans ATCC 23270 was 17Á1 3Á4%. The strain TJ 330 represents a high-af®nity bacterium which can effectively remove low CS 2 concentrations in an acid environment. These properties can be utilized in biotechnological puri®cation applications.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 79%

Physiology and taxonomy of Thiobacillus strain TJ330, which oxidizes carbon disulphide (CS2)

Hartikainen¹,

Ruuskanen²,

Räty

et al. 2000

J Appl Microbiol

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“…Various Thiobacillus species and Thiothrix ramosa have been described to use carbon disulfide as sole energy source for growth [23]. Various Thiobacillus species and Thiothrix ramosa have been described to use carbon disulfide as sole energy source for growth [23].…”

Section: Discussionmentioning

confidence: 99%

Volatile Organic Sulfur Compounds in a Meromictic Alpine Lake

Fritz¹,

Bachofen²

2000

Acta hydrochim. hydrobiol.

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“…Several bacterial species that can grow chemolithoautotrophically on CS 2 at neutral pH have been identified from soil, sludge, and freshwater habitats (8)(9)(10)(11)(12). At acidic pH, so far only some (Acidi)thiobacillus strains, isolated from hot springs and volcanic areas, were shown to be able to use CS 2 (8,13).…”

mentioning

confidence: 99%

Bacterial CS2 Hydrolases from Acidithiobacillus thiooxidans Strains Are Homologous to the Archaeal Catenane CS2 Hydrolase

Smeulders

Pol

Venselaar³

et al. 2013

Journal of Bacteriology

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c Carbon disulfide (CS 2 ) and carbonyl sulfide (COS) are important in the global sulfur cycle, and CS 2 is used as a solvent in the viscose industry. These compounds can be converted by sulfur-oxidizing bacteria, such as Acidithiobacillus thiooxidans species, to carbon dioxide (CO 2 ) and hydrogen sulfide (H 2 S), a property used in industrial biofiltration of CS 2 -polluted airstreams. We report on the mechanism of bacterial CS 2 conversion in the extremely acidophilic A. thiooxidans strains S1p and G8. The bacterial CS 2 hydrolases were highly abundant. They were purified and found to be homologous to the only other described (archaeal) CS 2 hydrolase from Acidianus strain A1-3, which forms a catenane of two interlocked rings. The enzymes cluster in a group of ␤-carbonic anhydrase (␤-CA) homologues that may comprise a subclass of CS 2 hydrolases within the ␤-CA family. Unlike CAs, the CS 2 hydrolases did not hydrate CO 2 but converted CS 2 and COS with H 2 O to H 2 S and CO 2 . The CS 2 hydrolases of A. thiooxidans strains G8, 2Bp, Sts 4-3, and BBW1, like the CS 2 hydrolase of Acidianus strain A1-3, exist as both octamers and hexadecamers in solution. The CS 2 hydrolase of A. thiooxidans strain S1p forms only octamers. Structure models of the A. thiooxidans CS 2 hydrolases based on the structure of Acidianus strain A1-3 CS 2 hydrolase suggest that the A. thiooxidans strain G8 CS 2 hydrolase may also form a catenane. In the A. thiooxidans strain S1p enzyme, two insertions (positions 26 and 27 [PD] and positions 56 to 61 [TPAGGG]) and a nine-amino-acid-longer C-terminal tail may prevent catenane formation.

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Degradation of carbondisulphide by a Thiobacillus isolate

Cited by 23 publications

References 18 publications

Physiology and taxonomy of Thiobacillus strain TJ330, which oxidizes carbon disulphide (CS2)

Physiology and taxonomy of Thiobacillus strain TJ330, which oxidizes carbon disulphide (CS2)

Volatile Organic Sulfur Compounds in a Meromictic Alpine Lake

Bacterial CS2 Hydrolases from Acidithiobacillus thiooxidans Strains Are Homologous to the Archaeal Catenane CS2 Hydrolase

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