Volatile Organic Sulfur Compounds in a Meromictic Alpine Lake

Fritz, Markus; Bachofen, Reinhard

doi:10.1002/1521-401x(20004)28:4<185::aid-aheh185>3.0.co;2-v

Cited by 27 publications

(12 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is also unlikely that epilimnetic organisms would suffer from strong oxidative stress, because they frequently experience high oxygen concentration and have multiple mechanisms to cope with it (22). Although conversion of methylated compounds such as methanethiol to methane can be bioenergetically favorable for methanogenic Archaea (13), neither methanethiol nor other methylated compounds are commonly found in high concentrations in oxygenated epilimnion in lakes (23)(24)(25). In our experiments, the addition of neither inorganic phosphate nor methylated compounds affected methane production; hence, pelagic methanogenesis in Lake Stechlin did not seem to depend on phosphate or methylated substrates like other systems (12,13).…”

Section: Discussionmentioning

confidence: 99%

Microbial methane production in oxygenated water column of an oligotrophic lake

Grossart

Frindte

Dziallas

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

284

328

View full text Add to dashboard Cite

The prevailing paradigm in aquatic science is that microbial methanogenesis happens primarily in anoxic environments. Here, we used multiple complementary approaches to show that microbial methane production could and did occur in the well-oxygenated water column of an oligotrophic lake (Lake Stechlin, Germany). Oversaturation of methane was repeatedly recorded in the well-oxygenated upper 10 m of the water column, and the methane maxima coincided with oxygen oversaturation at 6 m. Laboratory incubations of unamended epilimnetic lake water and inoculations of photoautotrophs with a lake-enrichment culture both led to methane production even in the presence of oxygen, and the production was not affected by the addition of inorganic phosphate or methylated compounds. Methane production was also detected by in-lake incubations of lake water, and the highest production rate was 1.8-2.4 nM·h −1 at 6 m, which could explain 33-44% of the observed ambient methane accumulation in the same month. Temporal and spatial uncoupling between methanogenesis and methanotrophy was supported by field and laboratory measurements, which also helped explain the oversaturation of methane in the upper water column. Potentially methanogenic Archaea were detected in situ in the oxygenated, methane-rich epilimnion, and their attachment to photoautotrophs might allow for anaerobic growth and direct transfer of substrates for methane production. Specific PCR on mRNA of the methyl coenzyme M reductase A gene revealed active methanogenesis. Microbial methane production in oxygenated water represents a hitherto overlooked source of methane and can be important for carbon cycling in the aquatic environments and water to air methane flux. epilimnic methane peak | methanogens A lthough methane makes up <2 parts per million by volume (ppmv) of the atmosphere, it accounts for 20% of the total radiative forcing among all long-lived greenhouse gases (1). In the aquatic environments, methane is also an important substrate for microbial production (2). The prevailing paradigm is that microbial methanogenesis occurs primarily in anoxic environments (3, 4). A commonly observed paradox is methane accumulation in well-oxygenated waters (2, 5), which is often assumed to be the result of physical transport from anoxic sediment and water (6-8) or in situ production within microanoxic zones (9-11). Two recent studies challenged this paradigm and suggested that microbes in oligotrophic ocean can metabolize methylated compounds and release methane even aerobically (12, 13). These claims are not without caveats, because the amounts of methylated compounds added [1-10 μM methylphosphonate (12) and 50 μM dimethyl sulfoniopropionate (13)] were far higher than their environmental concentrations, and therefore, the ecological relevance remains obscure. Moreover, dissolved oxygen (DO) was not monitored during the long incubation (5-6 d), and the possibility that the experimental setups had become anoxic before methane production could not be dismissed.* Despite the unce...

show abstract

Section: Discussionmentioning

confidence: 99%

Microbial methane production in oxygenated water column of an oligotrophic lake

Grossart

Frindte

Dziallas

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

284

328

View full text Add to dashboard Cite

show abstract

“…It is about 850 m long and 420 m wide, with a maximum depth of 21 m and a permanent chemocline found between 9 and 14 m. The lower layer is rich in dissolved salts brought by sulfurous sublacustrine springs. Several biogeochemical and molecular studies have been carried out in this lake (Wagener et al 1990, Putschew et al 1996, Schanz et al 1998, Bosshard et al 2000, Fritz & Bachofen 2000, Tonolla et al 2000, reporting blooms of the purple sulfur bacteria Chromatium okenii and Amoebobacter purpureus at the chemocline.…”

Section: Methodsmentioning

confidence: 99%

Composition and temporal dynamics of planktonic archaeal assemblages from anaerobic sulfurous environments studied by 16S rDNA denaturing gradient gel electrophoresis and sequencing

Casamayor¹,

Muyzer²,

Pedrós-Alió³

2001

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

The planktonic archaeal assemblages of several anaerobic, sulfide-rich, aquatic environments were analyzed in space and time by PCR-denaturing gradient gel electrophoresis and sequencing of 16S rRNA gene fragments. The systems were sampled in different years between 1992 and 1998. PCR products were obtained directly from the original DNA without previous nested amplification and yielded successful fingerprints with mostly sharp bands in the gel. Nineteen samples from the anaerobic hypolimnia of 8 lakes and 1 coastal lagoon in NE Spain, Mallorca and Switzerland were compared and a temporal survey was carried out in one of the lakes (Lake Vilar). Between 4 and 14 well-defined bands appeared. All the sequenced bands belonged to Archaea. Although most of the water bodies shared the same climatic conditions and presence of sulfide, the limnological parameters were different among them and different fingerprints were observed in different lakes. Euryarchaeota, i.e., methanogen-and thermoplasma-related sequences, appeared in all the samples but crenarchaeota were recovered only from Lake Vilar. A temporal shift in the predominant members of the archaeal assemblage from crenarchaeota to members of the cluster of thermoplasmales and relatives took place in Lake Vilar between February and June. Sequences related to thermoplasmales and crenarchaeota were distantly related to cultured strains (81% similarity in 16S rDNA) and clustered with branches represented only by environmental clones, whereas sequences related to methanogens grouped with a sequence from an endosymbiont of 1 anaerobic ciliate. A new branch of freshwater euryarchaeota appeared within the cluster of thermoplasmales and relatives. Our study indicates the presence of dynamic archaeal populations in the water column of nonthermophilic, sulfide-rich environments, further extending the diversity and distribution of Archaea in nature. The temporal shift in community composition in Lake Vilar suggests that Archaea grow under in situ conditions. If this is the case, Archaea would be active players in the anaerobic biogeochemical cycles of these environments.

show abstract

“…Additional work by Higgins et al, (2006) on municipal waste water activated sludge systems indicated the formation of DMDS could be an abiotic process that converts methanethiol to DMDS in the presence of oxygen and a metal catalyst. Fritz and Bachofen (2000) have also suggested this abiotic pathway for the formation of DMDS and DMTS in natural, freshwater systems. Our work suggests the controlling factor for emission to the atmosphere is water solubility regardless of the pathway by which volatile organic sulfur compounds are formed.…”

Section: Treatment Effects On Vocmentioning

confidence: 96%

VOC emissions from beef feedlot pen surfaces as affected by within-pen location, moisture and temperature

Woodbury

Gilley

Parker

et al. 2015

Biosystems Engineering

View full text Add to dashboard Cite

Feedlot Gas chromatographyOdour VOC A laboratory study was conducted to evaluate the effects of pen location, moisture, and temperature on emissions of volatile organic compounds (VOC) from surface materials obtained from feedlot pens where beef cattle were fed a diet containing 30% wet distillers grain plus solubles. Surface materials were collected from the feed trough (bunk), drainage, and raised areas (mounds) within three feedlot pens. The surface materials were mixed with water to represent dry, wet, or saturated conditions and then incubated at temperatures of 5, 15, 25 and 35 C. A wind tunnel and gas chromatograph-mass spectrometer were used to collect and quantify emissions of eight volatile fatty acids (VFAs), five aromatics and two sulfur-containing compounds. Pen location significantly (P < 0.05) affected measurements of 10 of the VOC with the largest values occurring for materials collected near the mound area. The largest VFA and aromatic emissions resulted for the dry moisture condition while wet and saturated conditions produced the largest sulfide emissions.Temperature affected emission of each VOC except indole, with values generally increasing as temperature increased. Odour activity value (OAV), which was the ratio of measured concentration of a single compound normalised to the odour threshold for that compound, was calculated for each compound. Four VFAs contributed 7.5% of the total OAV but only one aromatic, 4-methylphenol, was a major contributor to total OAV at 2.5%.In comparison, sulfide compounds contributed 87.3% of the total OAV. This research shows VOC emissions are affected by pen location, moisture condition, and temperature.Published by Elsevier Ltd on behalf of IAgrE. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Abbreviations: AFOs, animal feeding operations; DMDS, dimethyl disulfide; DMTS, dimethyl trisulfide; OAV, odour activity value; VFAs, volatile fatty acids; VOCs, volatile organic compounds; WDGS, wet distillers grains with solubles. * USDA is an equal opportunity provider and employer. The mention of trade names of commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. ScienceDirect jo urnal homepage: www .e lsev ie r.com/ locate/issn/153 75110 b i o s y s t e m s e n g i n e e r i n g 1 3 4 ( 2 0 1 5 ) 3 1 e4 1 http://dx.

show abstract

Volatile Organic Sulfur Compounds in a Meromictic Alpine Lake

Cited by 27 publications

References 30 publications

Microbial methane production in oxygenated water column of an oligotrophic lake

Microbial methane production in oxygenated water column of an oligotrophic lake

Composition and temporal dynamics of planktonic archaeal assemblages from anaerobic sulfurous environments studied by 16S rDNA denaturing gradient gel electrophoresis and sequencing

VOC emissions from beef feedlot pen surfaces as affected by within-pen location, moisture and temperature

Contact Info

Product

Resources

About