Degradation of a Plasticizer, di-n-Butylphthalate by Delftia sp. TBKNP-05

Patil, K. Neelakanteshwar; Kundapur, Rajesh R.; Shouche, Yogesh S.; Karegoudar, T. B.

doi:10.1007/s00284-005-0258-9

Cited by 39 publications

(23 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The initial step in the metabolism of phthalate esters is hydrolysis of ester bond by esterase/hydrolase to yield monoesters and then phthalate isomer [49,66,71,78,83,100]. Esterases/hydrolase are inducible and show broad substrate specifi city [10,14,78,83,102], while few are specifi c [103].…”

Section: Esterase/hydrolasementioning

confidence: 99%

See 1 more Smart Citation

Bacterial degradation of phthalate isomers and their esters

Vamsee-Krishna

Phale

2008

Indian J Microbiol

View full text Add to dashboard Cite

Phthalate isomers and their esters are used heavily in various industries. Excess use and leaching from the product pose them as major pollutants. These chemicals are toxic, teratogenic, mutagenic and carcinogenic in nature. Various aspects like toxicity, diversity in the aerobic bacterial degradation, enzymes and genetic organization of the metabolic pathways from various bacterial strains are reviewed here. Degradation of these esters proceeds by the action of esterases to form phthalate isomers, which are converted to dihydroxylated intermediates by specifi c and inducible phthalate isomer dioxygenases. Metabolic pathways of phthalate isomers converge at 3,4-dihydroxybenzoic acid, which undergoes either ortho-or meta-ring cleavage and subsequently metabolized to the central carbon pathway intermediates. The genes involved in the degradation are arranged in operons present either on plasmid or chromosome or both, and induced by specifi c phthalate isomer. Understanding metabolic pathways, diversity and their genetic regulation may help in constructing bacterial strains through genetic engineering approach for effective bioremediation and environmental clean up.

show abstract

Section: Esterase/hydrolasementioning

confidence: 99%

“…Esterases/hydrolase are inducible and show broad substrate specifi city [10,14,78,83,102], while few are specifi c [103]. The enzyme is either monomeric (molecular weight, 56 kDa) or dimeric (monomer molecular weight, 31 kDa or 27 kDa) [1,72].…”

Section: Esterase/hydrolasementioning

confidence: 99%

Bacterial degradation of phthalate isomers and their esters

Vamsee-Krishna

Phale

2008

Indian J Microbiol

View full text Add to dashboard Cite

show abstract

“…Xu et al (2005) studied the biodegradation of an endocrine-disrupting chemical di-n-butyl phthalate ester by Pseudomonas uorescens B-1. Patil et al (2006) investigated the degradation of DBP by Delftia sp. TBKNP-05.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of di-n-butyl phthalate in sequencing batch reactor bioaugmented with Micrococcus sp. and the bacterial community analysis

Yang

Wang

2014

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

Di-n-butyl phthalate is widely used as plasticizer, which has been listed as priority pollutant due to its toxic and ubiquitous characteristics. It is difficult to remove by the conventional wastewater treatment processes. In this paper, the feasibility of using Micrococcus sp. to bioaugment a sequencing batch reactor for degrading di-n-butyl phthalate (DBP) was investigated. The terminal restriction fragment length polymorphisms (T-RFLP) were used to analyze the variation of microbial community in the reactor. The experimental results showed that for the bioaugmented reactor, the removal efficiency of DBP was about 85 % as compared to 25 % of the control reactor when initial DBP concentration was 100 mg/L. The bioaugmentation not only enhanced the removal efficiency of target compound, but also shortened the start-up time of the reactor. The kinetics of DBP degradation conformed to the first-order model in both reactors. The T-RFLP analysis indicated the bacterial community changes in the acclimated activated sludge and the introduced Micrococcus sp. during the operational process.

show abstract

“…The detection of MBP as intermediate of DBP degradation in this work is consistent with previous reports. However, the most frequently reported products such as PA and PCA [13] are not detected. CHANG et al [14] pointed out that the high degradation rate of DBP might be the reason for that PA was not detected by GC/MS.…”

Section: Dbp Biodegradationmentioning

confidence: 95%

Aerobic biodegradation of di-n-butyl phthalate by Xiangjiang River sediment and microflora analysis

Zhou

Lin

et al. 2009

J. Cent. South Univ. Technol.

View full text Add to dashboard Cite

Di-n-butyl phthalate (DBP), one of phthalate acid esters (PAEs), was investigated to determine its biodegradation rate using Xiangjiang River sediment and find potential DBP degraders in the enrichment culture of the sediment. The sediment sample was incubated with an initial concentration of DBP of 100 mg/L for 5 d. The biodegradation rate of DBP was detected using HPLC and the degraded products were analyzed by GC/MS. Subsequently, the microbial diversity of the enrichment culture was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results reveal that almost 100% of DBP is degraded after merely 3 d, generating two main degraded products: mono-butyl phthalate (MBP) and 9-octadecenoic acid. After a six-month enrichment period under the pressure of DBP, the dominant family in the final enrichment culture is clustered with the Comamonas sp., the remaining are affiliated with Sphingomonas sp., Hydrogenophaga sp., Rhizobium sp., and Acidovorax sp. The results show the potential of these bacteria to be used in the bioremediation of DBP in the environment.

show abstract

Degradation of a Plasticizer, di-n-Butylphthalate by Delftia sp. TBKNP-05

Cited by 39 publications

References 21 publications

Bacterial degradation of phthalate isomers and their esters

Bacterial degradation of phthalate isomers and their esters

Biodegradation of di-n-butyl phthalate in sequencing batch reactor bioaugmented with Micrococcus sp. and the bacterial community analysis

Aerobic biodegradation of di-n-butyl phthalate by Xiangjiang River sediment and microflora analysis

Contact Info

Product

Resources

About