Degradation of 4-hydroxyphenylacetic acid by Trichosporon cutaneum

Sparnins, Velta L.; Anderson, J J; Omans, J; Dagley, S.

doi:10.1128/jb.136.1.449-451.1978

Cited by 19 publications

(9 citation statements)

References 16 publications

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“…Organism and cell extracts. The organism, a strain of T. cutaneum used in previous work (17), is able to utilize L-tyrosine and certain other aromatic compounds, such as phenol and 4-hydroxybenzoic, phenylacetic, and 4-hydroxyphenylacetic acids, as main carbon sources. Shake cultures were grown at 300C in a medium that contained (per liter): Na2HPO4, (or succinic acid), 0.35 g. The pH of the medium was 6.4.…”

Section: Methodsmentioning

confidence: 99%

Catabolism of L-tyrosine in Trichosporon cutaneum

Sparnins¹,

Burbee²,

Dagley³

1979

J Bacteriol

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Protocatechuic acid was a catabolite in the degradation of L-tyrosine by Trichosporon cutaneum. Intact cells oxidized to completion various compounds proposed as intennediates in this conversion, but they did not readily oxidize catabolites of the homogentisate and homoprotocatechuate metabolic pathways, which are known to function in other organisms. Cell extracts converted tyrosine first to 4-hydroxycinnamic acid and then to 4-hydroxybenzaldehyde and 4-hydroxybenzoic acid. The proposed hydration product of 4-hydroxycinnamic acid, namely, fl-(4-hydroxyphenyl)-hydracrylic acid, was synthesized chemically, and its enzymatic degradation to 4-hydroxybenzaldehyde was shown to be dependent upon additions of adenosine triphosphate and coenzyme A. The hydroxylase that attacked 4-hydroxybenzoate showed a specific requirement for reduced nicotinamide adenine dinucleotide phosphate. Protocatechuate, the product of this reaction, was oxidized by cell extracts supplemented with reduced nicotinamide adenine dinucleotide or, less effectively, with reduced nicotinamide adenine dinucleotide phosphate, but these extracts contained no ring fission dioxygenase for protocatechuate. Evidence is presented that the principal hydroxylation product of protocatechuate was hydroxyquinol, the benzene nucleus of which was cleaved oxidatively to give maleylacetic acid.

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Section: Methodsmentioning

confidence: 99%

Catabolism of L-tyrosine in Trichosporon cutaneum

Sparnins¹,

Burbee²,

Dagley³

1979

J Bacteriol

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“…An apparent exception to this second generalization is provided by T. cutaneum grown at the expense of 4-HPA. The ratio Hr/Hc for 3-HPA, which is not a catabolite of 4-HPA, is slightly less than for the established intermediate 3,4-HPA (14), and is significantly lower than for the growth substrate and isomer, 4-HPA. However, other observations suggest an explanation; namely, that 3-HPA is metabolized by the same enzymes as those used for 3,4-HPA.…”

Section: (I)mentioning

confidence: 77%

“…cutaneum (14). The low value (Hr/Hc = 0.191) obtained for 2,5-HPA using 4-HPA-grown cells also indicates that homogentisate (2,5-HPA) dioxygenase is used for 4-HPA catabolism, as well as for PA catabolism.…”

Section: Notes 527mentioning

confidence: 95%

Heat evolution of microbial catabolism: effects of monooxygenases

Anderson¹,

Dagley²

1980

J Bacteriol

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“…Trichosporon cutaneum is a yeast used for the treatment of waste liquid, and is very often isolated from activated sludge. In addition, some strains utilize phenol or its derivatives 1,4,[12][13][14] . Previously, we reported the isolation of T. cutaneum strain KUY-6A which grew on cyclohexanecarboxylic acid (CHCA) as a sole source of carbon, the degradation pathway of CHCA, and the utilization of aromatic compounds by the strain KUY-6A [7][8][9] .…”

mentioning

confidence: 99%

Degradation of Cyclopentanol by Trichosporon cutaneum Strain KUY-6A

et al. 2004

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Trichosporon cutaneum strain KUY-6A, a cyclohexanecarboxylic acid-utilizing yeast, was able to grow on cyclopentanol (CPOL) as a sole source of carbon and energy. Growth experiments revealed the strain, KUY-6A, could utilize up to 42 mM of CPOL with an optimum at 24 mM. Optimal growth was found between pH 4.0 to 9.0. The generation time under optimal growth conditions on CPOL was 3.0 h. Analysis indicated that cyclopentanone (CPON) and glutaric acid were intermediates of CPOL metabolism in strain KUY-6A. The results of growth and enzyme experiments are consistent with the degradation of CPOL via CPON, 5-valerolactone, 5-hydroxyvaleric acid, and glutaric acid.

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Degradation of 4-hydroxyphenylacetic acid by Trichosporon cutaneum

Abstract: Trichosporon cutaneum degraded 4-hydroxyphenylacetic acid to acetoacetic and malic acids. 3,4-Dihydroxyphenylacetic acid, an intermediate in the reaction sequence, underwent hydroxylation before the benzene ring was cleaved.

Cited by 19 publications

References 16 publications

Catabolism of L-tyrosine in Trichosporon cutaneum

Catabolism of L-tyrosine in Trichosporon cutaneum

Heat evolution of microbial catabolism: effects of monooxygenases

Degradation of Cyclopentanol by Trichosporon cutaneum Strain KUY-6A

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