Degradation of Cyclopentanol by Trichosporon cutaneum Strain KUY-6A

Abstract

Trichosporon cutaneum strain KUY-6A, a cyclohexanecarboxylic acid-utilizing yeast, was able to grow on cyclopentanol (CPOL) as a sole source of carbon and energy. Growth experiments revealed the strain, KUY-6A, could utilize up to 42 mM of CPOL with an optimum at 24 mM. Optimal growth was found between pH 4.0 to 9.0. The generation time under optimal growth conditions on CPOL was 3.0 h. Analysis indicated that cyclopentanone (CPON) and glutaric acid were intermediates of CPOL metabolism in strain KUY-6A. The results of growth and enzyme experiments are consistent with the degradation of CPOL via CPON, 5-valerolactone, 5-hydroxyvaleric acid, and glutaric acid.

Comparison of initial hydrolysis of the three dimethyl phthalate esters (DMPEs) by a basidiomycetous yeast, Trichosporon DMI-5-1, from coastal sediment

“…Error bars show standard deviations among the triplicate samples plant and animal materials, and human patients (Middelhoven et al 2004;Kaszycki et al 2006). Other studies have already shown that Trichosporon species have the ability to metabolize a wide range of recalcitrant compounds including benzene compounds (phenol, benzoic acid, phenylacetic acid, cinnamic acid, and their derivatives as well as some other monoaromatic compounds) (Middelhoven et al 1992;Middelhoven 1993), cyclopentanol (Iwaki et al 2004), biarylic compounds (biphenyl, dibenzofuran, and diphenyl ether) (Sietmann et al 2002), and PAHs (phenanthrene) (MacGillivray and Shiaris 1993). As DMPEs show similar structural characteristics with the above aromatic compounds, it was not surprising that Trichosporon species was capable of utilizing DMPEs as the sole source of carbon and energy in this study.…”