Degradation of 2,4,6-trichlorophenol by bacteria isolated from secondary sludge of a pulp and paper mill

Abstract

Bacterial strains capable of degrading trichlorophenol (2,4,6-TCP) were isolated from the secondary sludge of a pulp and paper mill and were characterized. These isolates were identifi ed as Planococcus rifi etoensis (CL4) and Bacillus pumilus (CL5), based on their 16S rRNA sequence analysis. These isolates were able to grow and utilize 2,4,6-TCP as their source of carbon as well as energy. HPLC analysis and stoichometric release of chloride in the medium confi rmed the degradation ability of these isolates. Removal effi ciency of 2,4,6-TCP by these isolates was discovered to be high. They were able to remove 90% of 2,4,6-TCP when grown at a concentration of 600 mg L 1 . Inoculation of these bacteria completely removed 2,4,6-TCP within 2 weeks from the sludge of the pulp and paper mill when supplemented at the rate of 100 mg L 1 . Absorbable Organic Halogen (AOX) and Extractable Organic Halogen (EOX) were signifi cantly reduced by 63% and 70% respectively from the sludge due to inoculation of these bacteria. These isolates have high potential to remove 2,4,6-TCP and may be used for removal of 2,4,6-TCP from pulp paper mill waste.

A Neoteric Double Perovskite Gd2NiMnO6Nanostructure Electrocatalyst for Augmented Detection of Ecological Pollutant 2, 4, 6 Trichlorophenol

“…5c. Hence, it shows that the reduction and oxidation peak value depends upon the pH value (3)(4)(5)(6)(7)(8)(9)(10)(11). The maximum peak current were raised at pH 3.…”

“…From this practical experiment, the fabricated GNMO/SPCE electrode has an excellent conductivity and magnify the rate of quantification of TCP. Hence, the preferred electrode shows a good potential towards the determination of TCP (2,4,6,Trichlorophenol).…”

“…Chlorophenol products have been widely used in the organic synthesis of insecticides, fungicides and herbicides, preservatives, printing, papermaking, dyeing industries and defoliant in the organic synthesis. [1][2][3][4][5][6][7][8] Among these Chlorophenol, 2,4,6-Trichlorophenol (TCP) is used in the preparation of pesticides, preservatives and dyes as an intensive organic chemical intermediate. 9 TCP has been used in wineries today to disinfect barrels, conserve wood, and cork.…”