Objective: The aim of this study was to investigate the effects of two different self-adjusting file (SAF) working times and three different chelating agents on the push-out bond strength of root canal fillings in oval root canals. Materials and Methods: Eighty mandibular premolars were selected and distributed into 8 groups (n=10): 4 min SAF (G1), 4 min SAF + 17% ethylenediaminetetraacetic acid (EDTA) (G2), 4 min SAF + 10% citric acid (CA) (G3), 4 min SAF + 1% peracetic acid (PAA) (G4), 6 min SAF (G5), 6 min SAF + 17% EDTA (G6), 6 min SAF + 10% CA (G7), and 6 min SAF + 1% PAA (G8). The root canals were filled using guttapercha and AH Plus root canal sealer, and the teeth were prepared for the push-out assessments using 1.0 mm thick root slices. Loading was performed with a universal testing machine at a speed of 0.5 mm/min; and then, collected data were analyzed statistically (α=0.05). Results: While the different chelating agents significantly affected the push-out bond strength of the root canal filling (p<0.05), the different SAF working times did not (p>0.05). There were no significant differences among the groups in the apical and middle thirds of the roots (p>0.05). In the coronal thirds, G6 and G8 indicated significantly greater bond strength values than G1 and G5 (p<0.05). Conclusion: According to the results, the 4 and 6 min SAF working times did not affect the bond strength of the root canal filling. PAA and EDTA can be recommended for root canal irrigation after SAF usage.