Deformability-selective particle entrainment and separation in a rectangular microchannel using medium viscoelasticity

Yang, Seungyoung; Lee, Sung-Sik; Ahn, Sungsook; Kang, Kyungran; Shim, Wooyoung; Lee, Gwang; Hyun, Kyu; Kim, Ju Min

doi:10.1039/c2sm07469a

Cited by 102 publications

(133 citation statements)

References 41 publications

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“…The lateral particle migration speed in a DNA solution depends upon the particle size a as denoted in equation (1) (Methods). This kind of behaviour was previously reported in synthetic polymer solutions 18,22,28,29 . We demonstrate a chromatographic separation of micron-sized particles utilizing this dependency of the lateral migration speed on particle size.…”

Section: Resultssupporting

confidence: 50%

“…Thus, the dilute DNA solution is considered to be a Boger fluid, which can be described with Oldroyd-B model 21 . By using a modelling procedure based on previous works 18,20,22 (see Methods for the modelling and direct comparative studies between the theoretical prediction and experimental data ( Supplementary Fig. S2)), the lateral particle migration speed (V) normalized with axial average velocity /uS, can be denoted as:…”

Section: Resultsmentioning

confidence: 99%

“…Lateral particle migration speed under non-Newtonian flow was predicted using previous semiempirical approaches 18,20,22,29 . Specifically, the dilute DNA solutions used in this work are Boger fluids, which are non-Newtonian elastic fluids with constant shear viscosities as shown in Supplementary Fig.…”

Section: Methodsmentioning

confidence: 99%

“…The dependency of Wi and the aspect ratio of particle size to channel height has been previously predicted 18,20,22,29 . The overlapping concentration c ?…”

Section: Polymer Contribution To the Viscosity Of A Polymer Solution mentioning

confidence: 99%

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DNA-based highly tunable particle focuser

et al. 2013

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DNA is distinguished by both long length and structural rigidity. Classical polymer theories predict that DNA enhances the non-Newtonian elastic properties of its dilute solution more significantly than common synthetic flexible polymers because of its larger size and longer relaxation time. Here we exploit this property to report that under Poiseuille microflow, rigid spherical particles laterally migrate and form a tightly focused stream in an extremely dilute DNA solution (0.0005 (w/v)%). By the use of the DNA solution, we achieve highly efficient focusing (499.5%) over an unprecedented wide range of flow rates (ratio of maximum to minimum flow rates B400). This highly tunable particle-focusing technique can be used in the design of cost-effective portable flow cytometers, high-throughput cell analysis and also for cell sorting by size. We demonstrate that DNA is an efficient elasticity enhancer, which originates from its unique structural properties.

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Section: Resultssupporting

confidence: 50%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The dependency of Wi and the aspect ratio of particle size to channel height has been previously predicted 18,20,22,29 . The overlapping concentration c ?…”

Section: Polymer Contribution To the Viscosity Of A Polymer Solution mentioning

confidence: 99%

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DNA-based highly tunable particle focuser

et al. 2013

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“…The off-center focusing has only been reported by numerical studies (κ ≥ 0.25) and macroscale experiments (κ = 0.6). 54,56 Most existing studies manipulate particles or cells with κ around 0.1 in square microchannels, 27,35,36,42,48,57,[59][60][61] and thus this off-center focusing was not discovered in these experiments. This off-center focusing is quite different from that in inertial flow Page 6 of 12 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 of Newtonian fluids.…”

Section: Acs Paragon Plus Environment Analytical Chemistrymentioning

confidence: 99%

Size-Based Separation of Particles and Cells Utilizing Viscoelastic Effects in Straight Microchannels

et al. 2015

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Viscoelasticity-induced particle migration has recently received increasing attention due to its ability to obtain high-quality focusing over a wide range of flow rates. However, its application is limited to low throughput regime since the particles can defocus as flow rate increases. Using an engineered carrier medium with constant and low viscosity and strong elasticity, the sample flow rates are improved to be one order of magnitude higher than those in existing studies. Utilizing differential focusing of particles of different sizes, here we present sheathless particle/cell separation in simple straight microchannels that possess excellent parallelizability for further throughput enhancement. The present method can be implemented over a wide range of particle/cell sizes and flow rates. We successfully separate small particles from larger particles, MCF-7 cells from red blood cells (RBCs), and Escherichia coli (E. coli) bacteria from RBCs in different straight microchannels. The proposed method could broaden the applications of viscoelastic microfluidic devices to particle/cell separation due to the enhanced sample throughput and simple channel design.Continuous manipulation and separation of particles and cells is important for a wide range of applications in biology, 1,2 medicine, 2,3 and industry. [4][5][6] Microfluidic systems have been proven to be promising tools for particle/cell manipulation with higher sensitivity and accuracy than their macroscale counterparts. The last decade has seen extensive development of microfluidic approaches for particle/cell manipulation that resort to immunocapture, 7 externally applied physical fields, [8][9][10][11][12][13][14][15][16][17][18] microfiltration, 19,20 gravitational sedimentation, 21 or deterministic lateral migration. 22,23 More recently, cross-streamline migration induced by the hydrodynamic effects of carrier media, such as inertia 24,25 and viscoelasticity, 26,27 has shown its promise for effective particle/cell manipulation without need of labeling and external force fields. Particles and cells can be separated based on the size-dependent nature of hydrodynamic forces. Briefly, the inertial lift scales as F a ∝ , where a is the particle diameter. There are several cell types of biological and clinical interest with separable size ranges: epithelial tumor cells (15-25 µm in diameter), blood cells (erythrocytes are 6-8 µm biconcave disks and peripheral blood lymphocytes are 7-10 µm in diameter), and bacteria (1-3 µm). Inertial migration in Newtonian fluids has been intensively studied and implemented in high-throughput label-free separation devices for cell separation. [28][29][30][31][32][33][34] Recently, particle migration induced by viscoelasticity has begun to attract increasing attention due to its simple focusing pattern and potential for achieving efficient focusing over a wide range of flow rates. 35,36 In a viscoelastic medium, elasticity coupled with non-negligible inertia will drive particles towards the channel centerline, whic...

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Manipulation of micro‐ and nanoparticles in viscoelastic fluid flows within microfluid systems

Manshadi

Mohammadi

Monfared

et al. 2019

Biotech & Bioengineering

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Manipulation of micro‐ and nanoparticles in complex biofluids is highly demanded in most biological and biomedical applications. A significant number of microfluidic platforms have been developed for inexpensive, rapid, accurate, and efficient particle manipulation. Due to the enormous potential of viscoelastic fluids (VEFs) for particle manipulation, various emerging microfluidic‐based VEFs techniques have been presented over the last decade. This review provides an intuitive understanding of VEF physics for particle separation in different microchannel geometries. Besides, active and passive VEF methods are critically reviewed, highlighting the potential and practical challenges of each technique for particle/cell focusing, sorting, and separation. The outcome of this study could enable recognizing deliverable VEF technology with the promising prospect in the manipulation of submicron biological samples (e.g., exosomes, DNA, and proteins).

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Deformability-selective particle entrainment and separation in a rectangular microchannel using medium viscoelasticity

Cited by 102 publications

References 41 publications

DNA-based highly tunable particle focuser

DNA-based highly tunable particle focuser

Size-Based Separation of Particles and Cells Utilizing Viscoelastic Effects in Straight Microchannels

Manipulation of micro‐ and nanoparticles in viscoelastic fluid flows within microfluid systems

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