Manipulation of micro‐ and nanoparticles in viscoelastic fluid flows within microfluid systems

Manshadi, Mohammad Karim Dehghan; Mohammadi, Mehdi; Monfared, Leila Karami; Sanati‐Nezhad, Amir

doi:10.1002/bit.27211

Cited by 29 publications

(25 citation statements)

References 78 publications

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“…Indeed, both forces are able to drive the flowing particles towards specific equilibrium positions depending on the channel geometry, flow rate, and properties of the suspending liquid, as well documented in several reviews. [72][73][74][75][76][77][78] Once the particles reached the equilibrium position, at sufficiently large concentrations, they begin to interact, possibly leading to the formation of crystal-like structures. In this stage, the overall dynamics depends upon the mutual distance between all the interacting particles.…”

Section: Particle Crystalsmentioning

confidence: 99%

Microfluidic formation of crystal-like structures

2021

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Section: Particle Crystalsmentioning

confidence: 99%

Microfluidic formation of crystal-like structures

2021

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“…In the case of a pressure-driven, non-Newtonian, viscoelastic flow, particles migrate towards the centreline of a microchannel due to a non-uniform distribution of the first normal stress between the centreline and the walls of microchannel 35 with non-negligible inertial effects in addition to the dominant elastic forces [36][37][38][39][40][41][42][43][44][45][46][47] . For 3D focusing of particles in rectangular geometries under similar conditions, the particles migrate towards the centreline and corners of the channel due to nonlinear effects of fluid inertia and fluid elasticity induced by the non-uniform distribution of normal stress.…”

Section: High Throughput Viscoelastic Particle Focusing and Separation In Spiral Microchannelsmentioning

confidence: 99%

High throughput viscoelastic particle focusing and separation in spiral microchannels

Kumar

Ramachandraiah

Iyengar

et al. 2021

Sci Rep

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Passive particle manipulation using inertial and elasto-inertial microfluidics have received substantial interest in recent years and have found various applications in high throughput particle sorting and separation. For separation applications, elasto-inertial microfluidics has thus far been applied at substantial lower flow rates as compared to inertial microfluidics. In this work, we explore viscoelastic particle focusing and separation in spiral channels at two orders of magnitude higher Reynolds numbers than previously reported. We show that the balance between dominant inertial lift force, dean drag force and elastic force enables stable 3D particle focusing at dynamically high Reynolds numbers. Using a two-turn spiral, we show that particles, initially pinched towards the inner wall using an elasticity enhancer, PEO (polyethylene oxide), as sheath migrate towards the outer wall strictly based on size and can be effectively separated with high precision. As a proof of principle for high resolution particle separation, 15 µm particles were effectively separated from 10 µm particles. A separation efficiency of 98% for the 10 µm and 97% for the 15 µm particles was achieved. Furthermore, we demonstrate sheath-less, high throughput, separation using a novel integrated two-spiral device and achieved a separation efficiency of 89% for the 10 µm and 99% for the 15 µm particles at a sample flow rate of 1 mL/min—a throughput previously only reported for inertial microfluidics. We anticipate the ability to precisely control particles in 3D at extremely high flow rates will open up several applications, including the development of ultra-high throughput microflow cytometers and high-resolution separation of rare cells for point of care diagnostics.

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“…Combination of inertial and elastic focusing at finite fluid inertia and elasticity regimes enables unique rigid particle or cell focusing at high throughput (Elasto‐inertial focusing). [ 7–11 ] Two non‐dimensional numbers, the channel Reynolds number ( Re ) and Weissenberg number ( Wi ), measure the inertial and elastic effect of the fluid, respectively:

R e = \frac{ρ_{normalf} V D_{normalh}}{η}

where ρ f is the fluid density; V is the average fluid velocity; D h is the hydraulic diameter of the channel; η is the dynamic viscosity of the fluid.

W i = λ \dot{γ} = λ 2 V / D_{normalh}

where λ is the relaxation time of the fluid;

\dot{γ}

is the characteristic shear rate.…”

Section: Theorectical Backgroundmentioning

confidence: 99%

Separation of Ultra‐High‐Density Cell Suspension via Elasto‐Inertial Microfluidics

Kwon

Choi

Han

2021

Small

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Separation of high‐density suspension particles at high throughput is crucial for many chemical, biomedical, and environmental applications. In this study, elasto‐inertial microfluidics is used to manipulate ultra‐high‐density cells to achieve stable equilibrium positions in microchannels, aided by the inherent viscoelasticity of high‐density cell suspension. It is demonstrated that ultra‐high‐density Chinese hamster ovary cell suspension (>26 packed cell volume% (PCV%), >95 million cells mL−1) can be focused at distinct lateral equilibrium positions under high‐flow‐rate conditions (up to 10 mL min−1). The effect of flow rates, channel dimensions, and cell densities on this unique focusing behavior is studied. Cell clarification is further demonstrated using this phenomenon, from 29.7 PCV% (108.1 million cells mL−1) to 8.3 PCV% (33.2 million cells mL−1) with overall 72.1% reduction efficiency and 10 mL min−1 processing rate. This work explores an extreme case of elasto‐inertial particle focusing where ultra‐high‐density culture suspension is efficiently manipulated at high throughput. This result opens up new opportunities for practical applications of high‐particle‐density suspension manipulation.

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Manipulation of micro‐ and nanoparticles in viscoelastic fluid flows within microfluid systems

Cited by 29 publications

References 78 publications

Microfluidic formation of crystal-like structures

Microfluidic formation of crystal-like structures

High throughput viscoelastic particle focusing and separation in spiral microchannels

Separation of Ultra‐High‐Density Cell Suspension via Elasto‐Inertial Microfluidics

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