Deformability based sorting of stored red blood cells reveals donor-dependent aging curves

Abstract

Cell sorting using microfluidic ratchets enables sensitive and consistent characterization of donor red blood cell deformability. Using this capability, we show the degradation of red blood cell deformability during cold storage is donor-dependent.

3D printed imaging platform for portable cell counting

“…Particle velocities can be calculated using track data obtained from particle counter code, using manual observation and PIVlab software. This PIV data can be used to obtain forces acting on particles and might further be useful in clinical applications like deformability-based cell sorting 41 or performing assays based on measuring the mechanical properties of biomaterials. 42 The future work on this topic will focus on improving the portability of our platform and expanding its diagnostic capabilities.…”

Concurrent Assessment of Deformability and Adhesiveness of Sickle Red Blood Cells by Measuring Perfusion of an Adhesive Artificial Microvascular Network

“…At present, these two RBC properties are typically assessed in vitro using separate devices, which entirely overlooks the complex interplay between the effect of deformability and adhesiveness on microvascular blood flow observed in vivo. Deformability is typically measured using classic ektacytometry (Connes et al, 2014(Connes et al, , 2018Parrow et al, 2017) and filtration assays (Smith et al, 1987), as well as various microfluidic devices (Burns et al, 2012;Picot et al, 2015;Guzniczak et al, 2018;Islamzada et al, 2020;Robidoux et al, 2020). Adhesiveness is evaluated by quantifying RBC adhesion to cultured endothelium or specific adhesion molecules under static (Smith et al, 1987) and flow conditions (Montes et al, 2002;Relevy et al, 2008;Alapan et al, 2016b).…”

“…The channels of microfluidic networks lined with endothelial cells invariably must have diameters larger than 15-20 µm to enable confluent culture; this trade-off omits the deformations RBCs experience in the smallest capillaries (Carden et al, 2017). Conversely, unlined microfluidic devices with narrow, capillarysize channels (some as small as 3-5 µm in diameter) designed to evaluate various aspects of RBC deformability do not assess adhesion (Glodek et al, 2010;Du et al, 2015;Picot et al, 2015;Burns et al, 2016;Islamzada et al, 2020;Man et al, 2020;Robidoux et al, 2020).…”

In vitro analysis of multiple blood flow determinants using red blood cell dynamics under oscillatory flow

“…0.025% v/v GA fixation increases RBC rigidity around 4 times. 35 Then, six additional blood samples (S-samples 1 to 6; S for stiffened) were prepared by suspending stiffened RBCs (47% v/v) in media 1 to 6, respectively. 70 kDa Dextran (Sigma-Aldrich) was added to three blood samples (healthy RBCs suspended in 1× PBS, 47% v/v) at different concentrations to change RBC aggregability (A-samples 1 to 3; A for aggregated).…”