In vitro analysis of multiple blood flow determinants using red blood cell dynamics under oscillatory flow

Abstract

<p>Flow behavior of blood is determined mainly by red blood cell (RBC) deformation and aggregation as well as blood viscoelasticity. These intricately interdependent parameters should be monitored by healthcare providers...</p>

Biosensing of Haemorheological Properties Using Microblood Flow Manipulation and Quantification

“…Based on Equation (9), λ t was expressed as a function of the interface ( β ). Unlike in previous studies [ 5 , 30 , 38 , 39 ], the present method does not assume that the time constant remains unchanged with respect to the interface. Thus, based on Equation (9), variations in λ t were obtained with respect to the interface under transient blood flow conditions (i.e., λ t = λ t [ β ]).…”

“…Previous studies have reported that coronary heart diseases significantly alter haemorheological properties [ 2 , 3 ]. Various haemorheological properties (i.e., viscosity, deformability, and aggregation) have been measured simultaneously to detect variations in RBCs and plasma [ 4 , 5 , 6 , 7 , 8 ]. Blood viscosity is quantified by measuring pressure-related parameters (i.e., interface relocation [ 9 , 10 ] or fluidic resistance variation [ 11 , 12 , 13 ]) under constant shearing blood flow.…”

“…Therefore, turning off the syringe pump or flow source, rather than decreasing the flow rate, is more effective [ 21 , 22 ]. Periodic on–off blood flow patterns are preferred to effectively obtain the biomechanical properties of blood [ 5 , 23 ]. Lee et al suggested a blood-flow switching mechanism in a bridge-shaped fluidic channel.…”

“…Kakei et al suggested an electrical impedance spectroscopy for monitoring RBC sedimentation in T-shaped container [ 28 ]. Elbuken et al measured two haemorheological properties (i.e., λ and AI) in the capillary tube by analysing the blood–air interface and blood image intensity after turning off the pump [ 5 ]. Using variations of the optical transmitted signal ( I ), λ was calculated by conducting a nonlinear curve fitting of I ( t ) = I 0 + I 1 exp (− t / λ ) [ 24 ].…”

Assessment of Blood Biophysical Properties Using Pressure Sensing with Micropump and Microfluidic Comparator

“…As the number of RBCs is much larger than the number of other cells or cell elements (i.e., white blood cells and platelets), most of the biophysical properties of blood samples have been determined by RBCs. Thus, most researchers have focused on RBCs and plasma protein [ 24 ].…”