Definitive Methods of Zygosity Determination in Twins: Relevance to Problems in the Biology of Twinning

Ga, Machin

doi:10.1017/s0001566000003688

Cited by 12 publications

(7 citation statements)

References 67 publications

(15 reference statements)

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“…Kato and Fujikik found that although certain anomalies occurred at a higher frequency in twins, the more common congenital anomalies did not occur at a signi®cantly higher frequency [19]. Machin calculated that if congenital anomalies due to placental or obstetric conditions were excluded, then congenital anomalies of monozygous twins did not occur at a higher rate [21].…”

Section: Discussionmentioning

confidence: 99%

Oesophageal atresia in twins

Orford

Glasson

Beasley

et al. 2000

Pediatric Surgery International

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The twin incidence is higher in infants with oesophageal atresia (OA) than in the general population. The purpose of this study was to review the twin OA information from five institutions and evaluate possible links between the development of OA and the twinning process. Data were compared, combined, and analysed. There was a total of 1,215 infants with OA, of whom 50 were from a twin pregnancy and 1 from a triplet pregnancy. Two sets of twins were concordant for OA. Mean birth weights and gestational ages were lower in the twin infants (P < 0.0005) and survival was lower in twins (65%, P < 0.005) than singletons. The anatomical variant of pure OA without fistula was seen in proportionally fewer twins (4%) than in singletons (7%). Multiple anomalies were present in 40% of twins compared with 33% of singletons, although this did not reach statistical significance. OA in our multicentre population was more common in twins. Several possible mechanisms are put forward to explain the apparent link between twinning and OA. Further analysis of this aspect of OA may aid in understanding the aetiology of this congenital anomaly.

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Section: Discussionmentioning

confidence: 99%

Oesophageal atresia in twins

Orford

Glasson

Beasley

et al. 2000

Pediatric Surgery International

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“…This technique can easily be applied to the fetuses as well, but this practice remains uncommon [10][11][12]. Zygosity does not influence the management of uncomplicated multiple pregnancies, but this information could be decisive in high-risk situations.…”

Section: Introductionmentioning

confidence: 99%

Prenatal Diagnosis of Zygosity by Fetal DNA Analysis, a Contribution to the Management of Multiple Pregnancies

Levy¹,

Mirlesse²,

Jacquemard³

et al. 2002

Fetal Diagn Ther

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Objective: To evaluate the contribution of prenatal diagnosis of zygosity by fetal DNA analysis to the management of multiple pregnancies. Methods: Between March 1999 and March 2000, 31 same-sex multiple pregnancies, were referred to our Institute during their second trimester to have amniocentesis done. Fetal DNA variants were studied in addition to karyotype. The main indications for amniocentesis were fetal growth discordance, fetal malformations or selective pregnancy termination. Zygosity results were compared to the first-trimester ultrasound diagnosis. Results: 21 out of the 31 multiple pregnancies were dizygous, and 10 out of the 31 pregnancies were monozygous. First-trimester ultrasound provided information on chorionicity only in 24 out of the 31 cases (77%). Of these 24 reports, 21 proved to be correct (87.5%). Moreover, in 2 cases among 14 pregnancies with growth discordance, DNA analysis ruled out a twin-to-twin transfusion syndrome, while ultrasound could not yield a conclusion. In 2 twin pregnancies with fetal malformations affecting one of the fetuses, DNA analysis made it possible to assess the risk for the other twin. In the 5 cases leading to selective termination, prenatal diagnosis of zygosity had a decisive value for the surviving fetus. Conclusion: Fetal DNA analysis is a simple and highly useful test for prenatal diagnosis of zygosity in the management of complicated multiple pregnancies.

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“…However, large sample quantities were required, and the procedures were costly [11]. Monozygosity determined by the more readily available but unreliable methods used in many past clinical twin studies must therefore be regarded as at best "probably acceptable" and at worst "not acceptable" [15]. These deficiencies have been overcome by the use of modern methods of DNA analysis, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…DNA-fingerprinting for the determination of twin zygosity at birth can be carried out with a small sample of chord blood [10,15] and has proved to be reliable and authoritative [3,4]. In a clinical study, we reported on a series of 13 twin pairs with clefts of the lip and palate out of our own collective of 1045 cleft patients between 1973 and 1991.…”

Section: Introductionmentioning

confidence: 99%

Use of Single- and Multi-Locus and Polymerase Chain Reaction Systems for Zygosity Determination - Clinical Application in Twins with Clefts of the Lip and Palate

Eufinger

Rand

Schütte

1995

Acta genet. med. gemellol.: twin res.

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Precision of zygosity determination in twins can be improved by the use of modern methods of DNA analysis. The clinical application of 4 single-(SLS) and 2 multi-locus (MLS), and 6 PCR (polymerase chain reaction) systems for zygosity determination in 12 twin pairs with oral clefts was compared with regard to the quality and quantity of sample material required and the probability of error in monozygosity determination. PCR systems proved to be superior to SLS or MLS, as DNA sampling is much more convenient, while its level of accuracy still fulfils clinical requirements. For this reason, PCR systems should be considered a basic method in modern clinical twin research.

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Definitive Methods of Zygosity Determination in Twins: Relevance to Problems in the Biology of Twinning

Cited by 12 publications

References 67 publications

Oesophageal atresia in twins

Oesophageal atresia in twins

Prenatal Diagnosis of Zygosity by Fetal DNA Analysis, a Contribution to the Management of Multiple Pregnancies

Use of Single- and Multi-Locus and Polymerase Chain Reaction Systems for Zygosity Determination - Clinical Application in Twins with Clefts of the Lip and Palate

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