Defining the sources of Paradox: DNA sequence markers for North American walnut (Juglans L.) species and hybrids

Potter, Daniel; Gao, F.; Baggett, Scott; McKenna, James R.; McGranahan, Gale H.

doi:10.1016/s0304-4238(01)00358-2

Cited by 51 publications

(33 citation statements)

References 20 publications

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“…The ITS1-5.8S-ITS2 region was amplified with the primers ITS5_bryo and ITS4_bryo (Stech & Frahm, 1999), the trnL-F region with the primers trnC and trnF (Taberlet et al, 1991) and trnD-T with the primers trnD and trnT (Demesure, Sodzi & Petit, 1995). For some specimens the trnD-T region was sequenced in two pieces with primers trnD and trnY_F, and trnT and trnY_R (Huttunen & Ignatov, unpublished data) to avoid problems with the poly-A/T repeat at end of the trnE-Y spacer (Potter et al, 2002;Shaw et al, 2005). PCR cycles included an initial denaturation with 5 min at 95uC, followed by 35 cycles of 30 s 95uC, 30 s 52uC, 1 min 30 s 72uC for the ITS region, 30-35 cycles of 45 s 95uC, 30 s 52uC, 1 min 30 s 72uC for the trnD-T, and 35 cycles of 1 min 95uC, 1 min 52uC, 1 min 72uC for the trnL-F, and a final elongation of 8 min at 72uC.…”

Section: Molecular Methodsmentioning

confidence: 99%

Rhynchostegium confusum, a new species from the Iberian Peninsula and its relation toR. confertumbased on morphological and molecular data

Cezón

Muñoz

Hedenäs

et al. 2010

Journal of Bryology

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Rhynchostegium confusum, a new species from Spain and the Iberian Peninsula is described. It is closely related to R. confertum, from which it differs in its noticeably wider stem leaves with wider and apically spurred costa, more flexuous branch leaves, and shorter seta. It also differs in its habit and habitat: the plants are tightly attached to siliceous rocks just over the water level in rivulets that dry out during the summer in the Mediterranean region. Molecular data show that the new species derives from R. confertum, but is clearly differentiated from the studied populations of the latter in a number of missing haplotypes.

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Section: Molecular Methodsmentioning

confidence: 99%

Rhynchostegium confusum, a new species from the Iberian Peninsula and its relation toR. confertumbased on morphological and molecular data

Cezón

Muñoz

Hedenäs

et al. 2010

Journal of Bryology

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“…The ITS region of the nuclear DNA includes three components: internal transcribed spacer 1, the 5.8S ribosomal RNA gene, and internal transcribed spacer 2 (Baldwin 1992). Sequence diversity among ITS1 alleles has been used to distinguish closely related species and for phylogenetic studies in many angiosperm families and fungi (Baldwin et al 1995;Potter et al 2002). Because the ITS region is highly conserved intraspecifically but variable among species, it is often used in taxonomy (Liston et al 1999).…”

mentioning

confidence: 99%

“…The trnT UGU -trnF GAA region, like other regions of the chloroplast, has been used extensively in plant systematic to differentiate among taxa (Olmstead and Palmer 1994;Won and Renner 2005). There are only a few species-specific markers for the Juglandaceae (Germain et al 1993;Potter et al 2002;McCleary et al 2009). Now that the regeneration of butternut has become a pressing concern, there is a need to develop concomitant molecular methods to identify nonhybrid butternut (Ostry and Woeste 2004).…”

mentioning

confidence: 99%

DNA markers identify hybrids between butternut (Juglans cinerea L.) and Japanese walnut (Juglans ailantifolia Carr.)

Zhao

Woeste

2010

Tree Genetics & Genomes

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Butternut (Juglans cinerea L.) is a temperate deciduous hardwood native to the eastern USA and southern Canada valued for its nuts and wood. Butternut's survival is threatened by butternut canker, a disease caused by the exotic fungus Sirococcus clavigignentijuglandacearum Nair, Kostichka & Kuntz. Field observations indicate that trees commonly called buartnut (a hybrid of butternut and its close congener Japanese walnut (Juglans ailantifolia × J. cinerea)) may be more resistant to butternut canker than is either parental species. Hybrids are difficult to distinguish morphologically from butternuts, and scientists have expressed concern over the possibility of range-wide genetic invasion by Japanese walnut via hybridization with butternut. We used pair-wise combinations of 40 random primers to screen bulked DNA pools of butternut, Japanese walnut, and buartnuts to identify genomic regions unique to Japanese walnut. We ultimately identified one ITS region marker, one chloroplast marker, one mitochondrial marker, and six nuclear markers. The utility of the markers for identifying hybrids was tested and verified using more than 190 genotypes. The markers will be used to identify buartnut hybrids based on the presence of introgressed genomic fragments inherited from Japanese walnut. We confirmed that hybrids have a complex genetic history and present features of the parental species in all possible combinations. These results will assist in the identification and testing of (non-hybrid) butternut for breeding and reintroduction of the species to its former habitats.

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“…Understanding the nature and origins of hybrids is important to breeders, ecologists, and taxonomists. DNA markers are the most commonly used molecular tools for identifying species and interspecific hybrids, and marker systems describing hybridization relevant to hardwood improvement have been described for Alnus (Prat 1988), Betula (Clausen 1979), Juglans (Potter et al 2002), Liriodendron (Wang 2003), Platanus (Santini 2001), Salix (Krstinic and Kajba 1997) Ulmus (Pinon et al 1999), and probably other genera as well.…”

Section: Molecular Marker Applicationsmentioning

confidence: 99%

Technological advances in temperate hardwood tree improvement including breeding and molecular marker applications

Pijut

Woeste

Vengadesan

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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Hardwood forests and plantations are an important economic resource for the forest products industry worldwide and to the international trade of lumber and logs. Hardwood trees are also planted for ecological reasons, for example, wildlife habitat, native woodland restoration, and riparian buffers. The demand for quality hardwood from tree plantations will continue to rise as the worldwide consumption of forest products increases. Tree improvement of temperate hardwoods has lagged behind that of coniferous species and hardwoods of the genera Populus and Eucalyptus. The development of marker systems has become an almost necessary complement to the classical breeding and improvement of hardwood tree populations for superior growth, form, and timber characteristics. Molecular markers are especially valuable for determining the reproductive biology and population structure of natural forests and plantations, and the identity of genes affecting quantitative traits. Clonal reproduction of commercially important hardwood tree species provides improved planting stock for use in progeny testing and production forestry. Development of in vitro and conventional vegetative propagation methods allows mass production of clones of mature, elite genotypes or genetically improved genotypes.Genetic modification of hardwood tree species could potentially produce trees with herbicide tolerance, disease and pest resistance, improved wood quality, and reproductive manipulations for commercial plantations. This review concentrates on recent advances in conventional breeding and selection, molecular marker application, in vitro culture, and genetic transformation, and discusses the future challenges and opportunities for valuable temperate (or "fine") hardwood tree improvement.

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Defining the sources of Paradox: DNA sequence markers for North American walnut (Juglans L.) species and hybrids

Cited by 51 publications

References 20 publications

Rhynchostegium confusum, a new species from the Iberian Peninsula and its relation toR. confertumbased on morphological and molecular data

Rhynchostegium confusum, a new species from the Iberian Peninsula and its relation toR. confertumbased on morphological and molecular data

DNA markers identify hybrids between butternut (Juglans cinerea L.) and Japanese walnut (Juglans ailantifolia Carr.)

Technological advances in temperate hardwood tree improvement including breeding and molecular marker applications

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