DNA markers identify hybrids between butternut (Juglans cinerea L.) and Japanese walnut (Juglans ailantifolia Carr.)

Zhao, Peng; Woeste, Keith

doi:10.1007/s11295-010-0352-4

Cited by 48 publications

(37 citation statements)

References 41 publications

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“…If true, this could be important because J. aílantífolía is being used in butternut breeding programs as a potential source of resistance to the butternut canker pathogen (37). Our results support the general susceptibility of this species to G. mórbida, although some half-sibling families were resistant.…”

Section: Discussionsupporting

confidence: 69%

Susceptibility of Walnut and Hickory Species to Geosmithia morbida

et al. 2013

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Utley, C, Nguyen, T., Roubtsova, T, Coggeshall, M., Ford, T. N. 2013. Susceptibility of walnut and hickory species to Geosmithia mórbida. Plant Dis. 97:601-607.Thousand cankers disease (TCD) of walnut is a result of feeding in the phloem by the walnut twig beetle (WTB), Pityophthorus juglandis, and subsequent canker formation caused by Geosmithia mórbida around galleries. TCD has caused extensive morbidity and mortality to Juglans nigra in the western United States and, in 2010, was discovered in the eastern United States, where the tree is a highly valuable timber resource. WTB and G. mórbida also have been found in / regia orchards throughout major production areas in California, and the numbers of damaged trees are increasing. We tested the susceptibility of walnut and hickory species to G. mórbida in greenhouse and field studies. Carya illinoinensis, C. aquatica, and C. ovata were immune. All wal-nut species tested, including / ailantifolia, J. californica, J. cinérea, J. hindsii, J. major, J. mandshurica, J. microcarpa, J. nigra, and J. regia, developed cankers following inoculation with G. mórbida. J. nigra was the most susceptible, whereas / major, a native host of the WTB and, presumably, G. mórbida, had smaller and more superficial cankers. Canker formation differed among maternal half-sibling families of / nigra and / cinérea, indicating genetic variability in resistance to G. mórbida. Our inoculation studies with G. mórbida have corroborated many of the field observations on susceptibility of walnut and hickory species to TCD, although the ability of the WTB to successfully attack and breed in walnut is also an important component in TCD resistance.

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Section: Discussionsupporting

confidence: 69%

Susceptibility of Walnut and Hickory Species to Geosmithia morbida

et al. 2013

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“…All the DNA samples were stored at Evolutionary Botany Lab, Northwest University, Xi'an, China. High-quality genomic DNA was extracted using a modified CTAB method (Zhao and Woeste, 2011). The DNA concentration was quantified using a NanoDrop spectrophotometer (Thermo Scientific, Carlsbad, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Completion of the Chloroplast Genomes of Five Chinese Juglans and Their Contribution to Chloroplast Phylogeny

2017

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Juglans L. (walnuts and butternuts) is an economically and ecologically important genus in the family Juglandaceae. All Juglans are important nut and timber trees. Juglans regia (Common walnut), J. sigillata (Iron walnut), J. cathayensis (Chinese walnut), J. hopeiensis (Ma walnut), and J. mandshurica (Manchurian walnut) are native to or naturalized in China. A strongly supported phylogeny of these five species is not available due to a lack of informative molecular markers. We compared complete chloroplast genomes and determined the phylogenetic relationships among the five Chinese Juglans using IIumina sequencing. The plastid genomes ranged from 159,714 to 160,367 bp encoding 128 functional genes, including 88 protein-coding genes and 40 tRNA genes each. A complete map of the variability across the genomes of the five Juglans species was produced that included single nucleotide variants, indels (insertions and deletions), and large structural variants, as well as differences in simple sequence repeats (SSR) and repeat sequences. Molecular phylogeny strongly supported division of the five walnut species into two previously recognized sections (Juglans/Dioscaryon and Cardiocaryon) with a 100% bootstrap (BS) value using the complete cp genomes, protein coding sequences (CDS), and the introns and spacers (IGS) data. The availability of these genomes will provide genetic information for identifying species and hybrids, taxonomy, phylogeny, and evolution in Juglans, and also provide insight into utilization of Juglans plants.

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“…Fresh leaves were collected and dried with silica gel. Genomic DNA was extracted following the methods of Doyle and Doyle [23] and Zhao and Woeste [24] and was resuspended in 50 µL of water, diluted to 10 ng/µL and then stored at −20 • C. RNA-seq libraries were generated using NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, Beverly, MA, USA) following manufacturer's recommendations, and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from 3 µg total RNA using poly-T oligo-attached magnetic beads.…”

Section: Sample Collections Dna Extraction and Rna Extractionmentioning

confidence: 99%

De Novo Assembly and Characterization of Bud, Leaf and Flowers Transcriptome from Juglans Regia L. for the Identification and Characterization of New EST-SSRs

Dang

Zhang

et al. 2016

Forests

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Persian walnut (Juglans regia L.), valued for both its nut and wood, is an ecologically important temperate tree species native to the mountainous regions of central Asia. Despite its importance, there are still few transcriptomic resources in public databases for J. regia, limiting gene discovery and breeding. Here, more than 49.9 million sequencing reads were generated using Illumina sequencing technology in the characterization of the transcriptome of four J. regia organs (bud, leaf, female flowers, and male flowers). De novo assembly yielded 117,229 unigenes with an N50 of 1955 bp. Based on sequence similarity searches against known proteins, a total of 20,413 (17.41%) genes were identified and annotated. A set of 27,584 unigenes with SSR (simple sequence repeats) motifs were identified as potential molecular markers, and a sample of 77 of these EST-SSRs (express sequence tags) were further evaluated to validate their amplification and assess their polymorphism. Next, we developed 39 polymorphic microsatellite markers to screen 88 Persian walnut individuals collected from 11 populations. These markers and transcriptomic resources will be useful for future studies of population genetic structure, evolutionary ecology, and breeding of Persian walnut and other Juglans species.

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DNA markers identify hybrids between butternut (Juglans cinerea L.) and Japanese walnut (Juglans ailantifolia Carr.)

Cited by 48 publications

References 41 publications

Susceptibility of Walnut and Hickory Species to Geosmithia morbida

Susceptibility of Walnut and Hickory Species to Geosmithia morbida

Completion of the Chloroplast Genomes of Five Chinese Juglans and Their Contribution to Chloroplast Phylogeny

De Novo Assembly and Characterization of Bud, Leaf and Flowers Transcriptome from Juglans Regia L. for the Identification and Characterization of New EST-SSRs

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