Defining the identity of human adipose-derived mesenchymal stem cells

Abstract

Adipose-derived mesenchymal stem cells (ADMSCs) are an ideal population for regenerative medical application. Both the isolation procedure and the culturing conditions are crucial steps, since low yield can limit further cell therapies, especially when minimal adipose tissue harvests are available for cell expansion. To date, a standardized procedure encompassing both isolation sites and expansion methods is missing, thus making the choice of the most appropriate conditions for the preparation of ADMSCs controversial, especially in view of the different applications needed. In this study, we compared the effects of three different commercial media (DMEM, aMEM, and EGM2), routinely used for ADMSCs expansion, and two supplements, FBS and human platelet lysate, recently proven to be an effective alternative to prevent xenogeneic antibody transfer and immune alloresponse in the host. Notably, all the conditions resulted in being safe for ADMSCs isolation and expansion with platelet lysate supplementation giving the highest isolation and proliferation rates, together with a commitment for osteogenic lineage. Then, we proved that the high ADMSC hematopoietic supportive potential is performed through a constant and abundant secretion of both GCSF and SCF. In conclusion, this study further expands the knowledge on ADMSCs, defining their identity definition and offers potential options for in vitro protocols for clinical production, especially related to HSC expansion without use of exogenous cytokines or genetic modifications.

The Adipose Mesenchymal Stem Cell Secretome Inhibits Inflammatory Responses of Microglia: Evidence for an Involvement of Sphingosine-1-Phosphate Signalling

“…ADSCs can be isolated from adipose tissue biopsies by several protocols, different in time to digestion, enzyme type and concentration, and in culture media [28,29]. In addition, nonenzymatic procedures have been described [30][31][32].…”

Hair Follicle Morphogenesis in the Treatment of Mouse Full-Thickness Skin Defects Using Composite Human Acellular Amniotic Membrane and Adipose Derived Mesenchymal Stem Cells

“…ADMSCs express the mesenchymal and pluripotent markers [15, 16] as we examined, and compared with stem cells derived from other sources, ADMSCs have unique advantages of abundant sources, easy obtainment, high proliferative activity, and multidirectional differentiation potentiality and therefore have become common seed cells in tissue engineering [17, 18]. …”

Protein O-mannosylation is crucial for human mesencyhmal stem cells fate

“…Cell isolation and growth MSC from lipoaspirate (ADMSC), bone marrow aspirate (BMMSC), and umbilical cord blood (CBMSC) were obtained from healthy donors after informed consent [3,10,28]. MSC were propagated in alpha MEM supplemented with 20 % FBS (Life Technologies, Carlsbad, CA, USA).…”

“…MSC can be easily isolated from many tissues including adipose tissue [3], bone marrow [4], skeletal muscle [5], deciduous dental pulp [6], synovium [7], Wharton's jelly [8], umbilical cord [9] and umbilical cord blood [10]. In the last years, many reports on both experimental animal models and human clinical trials have shown that MSC may improve tissue repair by secreting different factors (cytokines, proteins, mRNA), either soluble or embedded in extracellular vesicles (EVs), able to regulate a broad range of biological activities including angiogenesis, wound repair, immunity, and defense, as well as neural activities [11,12].…”