Defining gene networks controlling the maintenance and function of the differentiation niche by an in vivo systematic RNAi screen

Gao, Yuan; Mao, Ying; Xu, Rong-Gang; Zhu, Ruibao; Zhang, Ming; Sun, Jin; Shen, Dan; Peng, Ping; Xie, Ting; Ni, Jian-Quan

doi:10.1016/j.jgg.2018.10.008

Cited by 6 publications

(9 citation statements)

References 64 publications

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“…The finding that somatic cells on most germline cysts are replaced by an independent epithelial cell population at an analogous developmental stage in both Drosophila and mouse ovaries suggests that cellular succession is functionally important and has been conserved in evolution. The transcriptomes of mouse BPG cells (Dataset S2) will make it easier to compare the early stages of ovarian follicle formation in mammals and invertebrates (29,93,94).…”

Section: Two Pregranulosa Cell Pathways Support Two Different Folliclementioning

confidence: 99%

“…In both mouse and Drosophila , primordial germ cells migrate to the gonadal primordium ( 24 , 25 ), and oocytes differentiate within interconnected cysts of meiotic germ cells with the assistance of nurse-like cells that transfer organelles ( 26 , 27 ). In both organisms, early PG cells generated from bipotential precursors express Wnts and signal to developing germ cells ( 15 – 19 , 28 , 29 ). In Drosophila , these early cells are termed “escort cells” (ECs) and arise from bipotential pupal gonadal “intermingled cells” ( 30 ).…”

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confidence: 99%

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Two distinct pathways of pregranulosa cell differentiation support follicle formation in the mouse ovary

Niu

Spradling

2020

Proc. Natl. Acad. Sci. U.S.A.

161

205

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We sequenced more than 52,500 single cells from embryonic day 11.5 (E11.5) postembryonic day 5 (P5) gonads and performed lineage tracing to analyze primordial follicles and wave 1 medullar follicles during mouse fetal and perinatal oogenesis. Germ cells clustered into six meiotic substages, as well as dying/nurse cells. Wnt-expressing bipotential precursors already present at E11.5 are followed at each developmental stage by two groups of ovarian pregranulosa (PG) cells. One PG group, bipotential pregranulosa (BPG) cells, derives directly from bipotential precursors, expresses Foxl2 early, and associates with cysts throughout the ovary by E12.5. A second PG group, epithelial pregranulosa (EPG) cells, arises in the ovarian surface epithelium, ingresses cortically by E12.5 or earlier, expresses Lgr5, but delays robust Foxl2 expression until after birth. By E19.5, EPG cells predominate in the cortex and differentiate into granulosa cells of quiescent primordial follicles. In contrast, medullar BPG cells differentiate along a distinct pathway to become wave 1 granulosa cells. Reflecting their separate somatic cellular lineages, second wave follicles were ablated by diptheria toxin treatment of Lgr5-DTR-EGFP mice at E16.5 while first wave follicles developed normally and supported fertility. These studies provide insights into ovarian somatic cells and a resource to study the development, physiology, and evolutionary conservation of mammalian ovarian follicles.

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Section: Two Pregranulosa Cell Pathways Support Two Different Folliclementioning

confidence: 99%

mentioning

confidence: 99%

Two distinct pathways of pregranulosa cell differentiation support follicle formation in the mouse ovary

Niu

Spradling

2020

Proc. Natl. Acad. Sci. U.S.A.

161

205

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“…In general, many of these signals act to sustain the cytoskeletal structure and/or dynamics of escort cell protrusions. It has also been postulated that most escort cell signals limit the range of BMP signals emanating from cap cells, which blocks the undifferentiated (stem cell) state and promotes germ cell differentiation and mitotic division (Gao et al, 2019).…”

Section: Paracrine Signaling Promotes Gsc Self-renewal and Daughter Cmentioning

confidence: 99%

“…As a result, many genetic mutants that abrogate female fertility were described morphologically with respect either to cell biology (i.e., are oocytes made and if so, are they made correctly) or to meiotic recombination (i.e., did chromosomes exchange information correctly). More recently, screens employing powerful genetic tools to generate mutant cells specifically in the germline or ovarian soma increased our knowledge of the number of genes and genetic networks that underlie oogenesis (Morris et al, 2003;Denef et al, 2008;Ni et al, 2011;Horne-Badovinac et al, 2012;Czech et al, 2013;Jagut et al, 2013;Yan et al, 2014;Ables et al, 2016;Cho et al, 2018;Gao et al, 2019). These studies revealed that many fundamental molecular networks, particularly those that underlie asymmetric cell division during embryogenesis, are reiterated during the earliest steps of oogenesis to shape oocyte development.…”

Section: Introductionmentioning

confidence: 99%

Coordinating Proliferation, Polarity, and Cell Fate in the Drosophila Female Germline

Hinnant

Merkle

Ables

2020

Front. Cell Dev. Biol.

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Gametes are highly specialized cell types produced by a complex differentiation process. Production of viable oocytes requires a series of precise and coordinated molecular events. Early in their development, germ cells are an interconnected group of mitotically dividing cells. Key regulatory events lead to the specification of mature oocytes and initiate a switch to the meiotic cell cycle program. Though the chromosomal events of meiosis have been extensively studied, it is unclear how other aspects of oocyte specification are temporally coordinated. The fruit fly, Drosophila melanogaster, has long been at the forefront as a model system for genetics and cell biology research. The adult Drosophila ovary continuously produces germ cells throughout the organism's lifetime, and many of the cellular processes that occur to establish oocyte fate are conserved with mammalian gamete development. Here, we review recent discoveries from Drosophila that advance our understanding of how early germ cells balance mitotic exit with meiotic initiation. We discuss cell cycle control and establishment of cell polarity as major themes in oocyte specification. We also highlight a germline-specific organelle, the fusome, as integral to the coordination of cell division, cell polarity, and cell fate in ovarian germ cells. Finally, we discuss how the molecular controls of the cell cycle might be integrated with cell polarity and cell fate to maintain oocyte production.

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“…39 CySCs have been proven to regulate the differentiation of GSCs in Drosophila testes. 7 Our study found that knockdown of 43 Additionally, Rho1 works in escort cells to promote GSCs progeny differentiation by maintaining EGFR signalling and preventing BMP signalling. 44 The ERK signalling pathway attenuates Rho1 activity to maintain the somatic escort cells' shape and function.…”

Section: Discussionmentioning

confidence: 69%

RpS13 controls the homeostasis of germline stem cell niche through Rho1‐mediated signals in the Drosophila testis

Wang

Chen

et al. 2020

Cell Proliferation

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Defining gene networks controlling the maintenance and function of the differentiation niche by an in vivo systematic RNAi screen

Cited by 6 publications

References 64 publications

Two distinct pathways of pregranulosa cell differentiation support follicle formation in the mouse ovary

Two distinct pathways of pregranulosa cell differentiation support follicle formation in the mouse ovary

Coordinating Proliferation, Polarity, and Cell Fate in the Drosophila Female Germline

RpS13 controls the homeostasis of germline stem cell niche through Rho1‐mediated signals in the Drosophila testis

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