RpS13 controls the homeostasis of germline stem cell niche through Rho1‐mediated signals in the <i>Drosophila</i> testis

Wang, Min; Chen, Xia; Wu, Yibo; Zheng, Qianwen; Chen, Wanyin; Yan, Yi–Dan; Luan, Xiaojin; Shen, Cong; Fang, Jie; Zheng, Bo; Yu, Jun

doi:10.1111/cpr.12899

Cited by 17 publications

(9 citation statements)

References 50 publications

(118 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To further explore the roles of CG6015 during germline differentiation, we analyzed morphological changes of fusomes using immunofluorescence staining. Fluorescence tomography imaging has shown that fusomes, which are connections and special structures among germ cells, undergo dynamic changes from a punctate to a branched morphology during germline differentiation 30 , 31 . In this study, we observed that undifferentiated germ cells, which were marked by DNA and Vasa, accumulated in Bam> CG6015 RNAi testes (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…We next investigated the maintenance of cyst cells using cyst cell markers (Zfh1 and Eya) under conditions of loss of CG6015 in spermatogonia. In control testes, Zfh1 could label cyst stem cells (CySCs) and the early stage of cyst cells, while Eya was the marker of mature cyst cells 31 , 33 . The decay of Zfh1 is necessary for promoting the differentiation of cyst cell lineage 33 .…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

CG6015 controls spermatogonia transit-amplifying divisions by epidermal growth factor receptor signaling in Drosophila testes

Zheng

et al. 2021

Cell Death Dis

Self Cite

View full text Add to dashboard Cite

Spermatogonia transit-amplifying (TA) divisions are crucial for the differentiation of germline stem cell daughters. However, the underlying mechanism is largely unknown. In the present study, we demonstrated that CG6015 was essential for spermatogonia TA-divisions and elongated spermatozoon development in Drosophila melanogaster. Spermatogonia deficient in CG6015 inhibited germline differentiation leading to the accumulation of undifferentiated cell populations. Transcriptome profiling using RNA sequencing indicated that CG6015 was involved in spermatogenesis, spermatid differentiation, and metabolic processes. Gene Set Enrichment Analysis (GSEA) revealed the relationship between CG6015 and the epidermal growth factor receptor (EGFR) signaling pathway. Unexpectedly, we discovered that phosphorylated extracellular regulated kinase (dpERK) signals were activated in germline stem cell (GSC)-like cells after reduction of CG6015 in spermatogonia. Moreover, Downstream of raf1 (Dsor1), a key downstream target of EGFR, mimicked the phenotype of CG6015, and germline dpERK signals were activated in spermatogonia of Dsor1 RNAi testes. Together, these findings revealed a potential regulatory mechanism of CG6015 via EGFR signaling during spermatogonia TA-divisions in Drosophila testes.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

CG6015 controls spermatogonia transit-amplifying divisions by epidermal growth factor receptor signaling in Drosophila testes

Zheng

et al. 2021

Cell Death Dis

Self Cite

View full text Add to dashboard Cite

show abstract

“…In Drosophila testes, the stem cell niche is studied as a model to mimic the formation of TGCTs. Drosophila provides a powerful genetically tractable system to study how germ cell differentiation has adapted in response to changes in external signals in testes [ 41 – 43 ]. Our study investigated roles of translation initiation factor eIF5 in Drosophila testes systemically and uncovered that CySC lineage eIF5 was essential for cyst cell differentiation, which then promoted germ cell differentiation via non-autonomous cell effects (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that CySC lineage cells lacking small ribosomal subunits, e.g., ribosomal protein S13 (RpS13), ribosomal protein S26 (RpS26), ribosomal protein S30 (RpS30), ribosomal protein S15Aa (RpS15Aa), and ribosomal protein S15Ab (RpS15Ab), lead to the accumulation of undifferentiated GSC-like masses [ 14 , 43 ]. In this study, we found that cyst cells lacking eIF5, eIF1A, and eIF2γ, which are key components of the pre-initiation complex, mimicked the phenotype of cells lacking several small ribosomal subunits for germ cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

Cyst stem cell lineage eIF5 non-autonomously prevents testicular germ cell tumor formation via eIF1A/eIF2γ-mediated pre-initiation complex

et al. 2022

Stem Cell Res Ther

Self Cite

View full text Add to dashboard Cite

Background Stem cell niche maintains stem cell population identity and is essential for the homeostasis of self-renewal and differentiation in Drosophila testes. However, the mechanisms of CySC lineage signals-mediated soma–germline communications in response to external stimuli are unclear. Methods Pre-initiation complex functions were evaluated by UAS-Gal4-mediated cell effects. RNA sequencing was conducted in NC and eIF5 siRNA-treated cells. Genetic interaction analysis was used to indicate the relationships between eIF5 and eIF1A/eIF2γ in Drosophila testes. Results Here, we demonstrated that in CySCs, translation initiation factor eIF5 mediates cyst cell differentiation and the non-autonomously affected germ cell differentiation process. CySCs lacking eIF5 displayed unbalanced cell proliferation and apoptosis, forming testicular germ cell tumors (TGCTs) during spermatogenesis. eIF5 transcriptional regulation network analysis identified multiple metabolic processes and several key factors that might be involved in germ cell differentiation and TGCT formation. Importantly, knockdown of eIF1A and eIF2γ, key components of pre-initiation complex, mimicked the phenotype of knocking down eIF5 in the stem cell niche of Drosophila testes. Genetic interaction analysis indicated that eIF5 was sufficient to rescue the phenotype of tumorlike structures induced by down-regulating eIF1A or eIF2γ in CySCs. Conclusions These findings demonstrated that CySC lineage eIF5, together with eIF1A or eIF2γ, mediates soma–germline communications for the stem cell niche homeostasis in Drosophila testes, providing new insights for the prevention of TGCTs.

show abstract

“…Immuno uorescence assays were conducted as previous descriptions 17,18,19,20 . Brie y, the slides containing target cells and tissues were incubated with primary and Alexa-Fluor secondary antibodies (Thermo Scienti c, Waltham, USA) orderly.…”

Section: Immuno Uorescencementioning

confidence: 99%

Long Non-coding RNA NRSN2-AS1 promotes ovarian cancer progression through targeting PTK2/β-catenin pathway

Shen,

Wu,

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

As a common malignant tumor among women, ovarian cancer poses a serious threat to their health. This study demonstrates that long non-coding RNA NRSN2-AS1 is over-expressed in ovarian cancer tissues using patient sample and tissue microarrays. In addition, NRSN2-AS1 is shown to promote ovarian cancer cell proliferation and metastasis both in vitro and in vivo. Mechanistically, NRSN2-AS1 stabilizes protein tyrosine kinase 2 (PTK2) to activate the β-catenin pathway via repressing MG-53-mediated ubiquitinated degradation of PTK2, thereby facilitating ovarian cancer progression. Rescue experiments verify the function of the NRSN2-AS1/PTK2/β-catenin axis and the effects of MG53 on this axis in ovarian cancer cells. In conclusion, this study demonstrates the key role of the NRSN2-AS1/PTK2/β-catenin axis for the first time and explores its potential clinical applications in ovarian cancer.

show abstract

RpS13 controls the homeostasis of germline stem cell niche through Rho1‐mediated signals in the Drosophila testis

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 17 publications

References 50 publications

CG6015 controls spermatogonia transit-amplifying divisions by epidermal growth factor receptor signaling in Drosophila testes

CG6015 controls spermatogonia transit-amplifying divisions by epidermal growth factor receptor signaling in Drosophila testes

Cyst stem cell lineage eIF5 non-autonomously prevents testicular germ cell tumor formation via eIF1A/eIF2γ-mediated pre-initiation complex

Long Non-coding RNA NRSN2-AS1 promotes ovarian cancer progression through targeting PTK2/β-catenin pathway

Contact Info

Product

Resources

About