Defining critical antibody titre in column agglutination method to guide fetal surveillance

Krishnan, Vinod; Shenoy, Veena; Sunny, Shilna; Thambiran, Ramanathan; Keezhattoor, Dhanya; Namdeo, Sushmita; Radhamany, K

doi:10.1016/j.transci.2020.102732

Cited by 4 publications

(7 citation statements)

References 12 publications

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“…We noticed a large disparity between titers obtained by CTT and GMA (in some cases, four dilutions: CTT = 2 and GMA = 32). Three to eight dilutions greater in GMA than in CTT were likewise reported by other authors when analyzing alloimmunized patients (Krishnan et al 2020;Novaretti et al 2003). Nonetheless, equivalent levels or one to two differences in dilution by the two methods were also detected (Finck et al 2013;Flesiopoulou et al 2020).…”

Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

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Rho(D) Immune Globulin (anti-D) Concentration-Time Curve After Antenatal Immunoprophylaxis in RhD-Negative Pregnant Women

Silva¹,

Santos²,

Quintana

et al. 2021

Preprint

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Objectives: To assess anti-D concentration through maternal indirect antiglobulin test (IAT) titers and scores after RhD-negative pregnant women receive antenatal anti-D until delivery. Methods: Cross-sectional study. Twenty-seven RhD-negative pregnant women were evaluated after receiving a prenatal anti-D dose around 28th week of pregnancy. Serial blood samples were collected at 3, 7, 21, 42, 63 and 84 days after anti-D administration. We performed IAT by conventional tube test (CTT) and gel microcolumn assay (GMA) to assess anti-D scores and titers. Statistical analyzes were performed using IBM SPSS Statistics software. Results: Anti-D had maximum scores values around 7 days after its administration, and scores were higher in GMA than CTT for all patients. Anti-D was detected at delivery in 59% of the participants by IAT performed in GMA. Overweight and obese pregnant women may present lower concentrations of anti-D. No correlation was found between maximum scores when comparing them with these variables: newborn RhD blood type; maternal-fetal ABO incompatibility and the number of pregnancies. Conclusions: Administering 300 μg of anti-D in 28th week of pregnancy should be compared in studies analyzing perinatal outcomes, since anti-D was not detectable in 41% of patients, suggesting a significant period without prophylaxis.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Rho(D) Immune Globulin (anti-D) Concentration-Time Curve After Antenatal Immunoprophylaxis in RhD-Negative Pregnant Women

Silva¹,

Santos²,

Quintana

et al. 2021

Preprint

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“…Three to eight dilutions greater in GMA than in CTT were likewise reported by other authors when analyzing alloimmunized patients. [14,15] Nonetheless, equivalent levels or one to two differences in dilution by the two methods were also detected. [16,17] Although it is not possible to differentiate through CTT or GMA which type of antibody is being titrated (whether from anti-D prophylaxis or due to alloimmunization) [18] the results of this study may be useful to corroborate this discernment.…”

Section: Discussionmentioning

confidence: 92%

“…In cases of titers smaller than 16 to 32, it is usually not correlated to adverse perinatal outcomes. It requires antenatal supervision, but less mandatory distinguishing between alloimmunization and anti-D. [14] Criteria such as these do not discriminate antibody source, since it is not possible to exclude an alloimmunized pregnant woman with low titers. On the other hand, in cases of titers higher than 64 in GMA and 8-16 in CTT there is a high probability of being an alloimmunization, since these levels were not reached in the titration of anti-D. [19] It is also important to monitor titers throughout pregnancy, re-analyzing samples after 3-4 weeks (whenever possible in parallel with previous sample), since if antibody were prophylactic, it is likely titer has declined, as demonstrated.…”

Section: Discussionmentioning

confidence: 99%

“…Logo, o acesso desses eritrócitos fetais ao sistema imune materno é bloqueado, limitando a sensibilização ao antígeno RhD. [14][15][16] No entanto, a incompatibilidade ABO não oferece proteção para o feto numa resposta imune secundária, uma vez que a sensibilização já ocorreu. 17 Além disso, acredita-se que a incompatibilidade ABO também atue na redução de imunização contra antígenos não-RhD.…”

Section: Introductionunclassified

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Curva da concentração de imunoglobulina anti-RhD após imunoprofilaxia pré-natal em gestantes RhD negativo

Silva¹

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Ao meu orientador, e ouso dizer amigo, Prof. Dr. Geraldo Duarte, por sua acolhida, confiança, paciência, disponibilidade, incentivo e carinho. Obrigada por possibilitar que eu aprendesse tanto ao seu lado! O senhor é um modelo de dedicação e paixão pelo ensino e pela atenção à saúde! À Dra. Flávia Leite, que apesar de não poder constar oficialmente como minha coorientadora, é incrivelmente merecedora desse título, por todo ensinamento e contribuição para meu crescimento.À minha professora, Gabriela Campos, por ter sido um anjinho ao cruzar meu caminho, acreditando em mim antes que eu pensasse ser possível chegar onde estou.Aos meus pais e ao meu irmão, por todo amor que demonstram ao apoiar, em todos os sentidos possíveis, as minhas escolhas e meus sonhos, desde sempre! Ao meu namorado, por todo carinho, e por compreender, incentivar e permanecer sempre ao meu lado em cada uma de minhas realizações.Aos meus amigos, que já faziam parte da minha vida e os que a pós-graduação me presenteou, por terem oferecido um momento de apoio, de conselho, de carinho, de torcida e de calmaria.Aos professores e funcionários de todos os cantinhos da USP de Ribeirão Preto em que me aventurei na busca de aprendizado, com destaque ao Departamento de Ginecologia e Obstetrícia, o qual nunca tinha imaginado antes poder ser parte, e fico muito grata por tê-lo escolhido.Ao Hemocentro de Ribeirão Preto, em especial a toda equipe dos Laboratórios de Imunohematologia de Receptores e Doadores, e de Citometria de Fluxo, por permitirem a execução dos experimentos, testes e análises deste estudo.

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Maternal mirror syndrome with foetal hydrops due to isoimunization by anti‐KPa antibodies: A case report and narrative literature review

Moreno

Abad

Pérez‐Corral

et al. 2022

Clinical Case Reports

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Defining critical antibody titre in column agglutination method to guide fetal surveillance

Cited by 4 publications

References 12 publications

Rho(D) Immune Globulin (anti-D) Concentration-Time Curve After Antenatal Immunoprophylaxis in RhD-Negative Pregnant Women

Rho(D) Immune Globulin (anti-D) Concentration-Time Curve After Antenatal Immunoprophylaxis in RhD-Negative Pregnant Women

Curva da concentração de imunoglobulina anti-RhD após imunoprofilaxia pré-natal em gestantes RhD negativo

Maternal mirror syndrome with foetal hydrops due to isoimunization by anti‐KPa antibodies: A case report and narrative literature review

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