Deficiency of ligase IV leads to reduced NHEJ, accumulation of DNA damage, and can sensitize cells to cancer therapeutics

Kumari, Nitu; Antil, Himanshu; Kumari, Susmita; Raghavan, Sathees C.

doi:10.1016/j.ygeno.2023.110731

Genomics

2023

DOI: 10.1016/j.ygeno.2023.110731

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Deficiency of ligase IV leads to reduced NHEJ, accumulation of DNA damage, and can sensitize cells to cancer therapeutics

Nitu Kumari,

Himanshu Antil,

Susmita Kumari

et al.

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2024

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Cited by 2 publications

References 97 publications

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Targeting senescent cells to reshape the tumor microenvironment and improve anticancer efficacy

Jiang,

Zhang,

Zhang

et al. 2024

Seminars in Cancer Biology

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Targeting senescent cells to reshape the tumor microenvironment and improve anticancer efficacy

Jiang,

Zhang,

Zhang

et al. 2024

Seminars in Cancer Biology

View full text Add to dashboard Cite

Crosstalk between BER and NHEJ in XRCC4-Deficient Cells Depending on hTERT Overexpression

Sergeeva,

Loshchenova,

Oshchepkov

et al. 2024

IJMS

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Targeting DNA repair pathways is an important strategy in anticancer therapy. However, the unrevealed interactions between different DNA repair systems may interfere with the desired therapeutic effect. Among DNA repair systems, BER and NHEJ protect genome integrity through the entire cell cycle. BER is involved in the repair of DNA base lesions and DNA single-strand breaks (SSBs), while NHEJ is responsible for the repair of DNA double-strand breaks (DSBs). Previously, we showed that BER deficiency leads to downregulation of NHEJ gene expression. Here, we studied BER’s response to NHEJ deficiency induced by knockdown of NHEJ scaffold protein XRCC4 and compared the knockdown effects in normal (TIG-1) and hTERT-modified cells (NBE1). We investigated the expression of the XRCC1, LIG3, and APE1 genes of BER and LIG4; the Ku70/Ku80 genes of NHEJ at the mRNA and protein levels; as well as p53, Sp1 and PARP1. We found that, in both cell lines, XRCC4 knockdown leads to a decrease in the mRNA levels of both BER and NHEJ genes, though the effect on protein level is not uniform. XRCC4 knockdown caused an increase in p53 and Sp1 proteins, but caused G1/S delay only in normal cells. Despite the increased p53 protein, p21 did not significantly increase in NBE1 cells with overexpressed hTERT, and this correlated with the absence of G1/S delay in these cells. The data highlight the regulatory function of the XRCC4 scaffold protein and imply its connection to a transcriptional regulatory network or mRNA metabolism.

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Deficiency of ligase IV leads to reduced NHEJ, accumulation of DNA damage, and can sensitize cells to cancer therapeutics

Cited by 2 publications

References 97 publications

Targeting senescent cells to reshape the tumor microenvironment and improve anticancer efficacy

Targeting senescent cells to reshape the tumor microenvironment and improve anticancer efficacy

Crosstalk between BER and NHEJ in XRCC4-Deficient Cells Depending on hTERT Overexpression

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