Defensive conditioning-related increase in AP-1 transcription factor in the rat cortex

Łukasiuk, Katarzyna; Savonenko, Alena; Nikolaev, Evgeni; Rydz, Marek; Kaczmarek, Leszek

doi:10.1016/s0169-328x(99)00045-5

Cited by 17 publications

(10 citation statements)

References 27 publications

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“…At present, experimental models of vibrissae stimulation described in the literature require immobilization and pain (Jablonska et al 1996;Lukasiuk et al 1999), anesthesia (Mack and Mack 1992), other artificial situations like a Lausanne stimulator (an electromagnetic coil with magnetic field bursts moving metal pieces glued to whiskers, Welker 1964;Welker et al 1989), or introduction of animals into a very elaborate environment (Montero 1997). We have developed simpler experimental systems to investigate the effects of tactile stimulation of vibrissae on gene expression.…”

mentioning

confidence: 99%

Tactile Experience Induces c-fos Expression in Rat Barrel Cortex

Filipkowski¹,

Rydz²,

Berdel³

et al. 2000

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Understanding gene expression that is responsive to sensory stimulation is central to elucidate molecular mechanisms underlying neuronal plasticity. In this study we demonstrate two new methods of stimulating whiskers that provide major sensory input to rat neocortex. In the first paradigm, animals were placed on the top of a cylinder and their vibrissae were brushed by hand. In the second paradigm, animals were placed for a brief period of time into a new, wired cage resulting in vibrissae stimulation when they explored the new environment. Both approaches induced c-Fos expression in barrel cortex corresponding to the stimulated vibrissae, especially in layer IV. Layers II/III and V/VI also showed c-Fos induction, but there were no detectable changes in layer VIb. The majority of c-Fos-expressing cells are probably not inhibitory neurons, because they do not show parvalbumin staining. Both paradigms, in contrast to the previous methods, are simple to use and do not require anesthesia, restraint of animals, or elaborate experimental setups.

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confidence: 99%

Tactile Experience Induces c-fos Expression in Rat Barrel Cortex

Filipkowski¹,

Rydz²,

Berdel³

et al. 2000

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“…DNA‐binding activity was measured with the EMSA method, as described by Lukasiuk et al . (1999), with slight modifications.…”

Section: Methodsmentioning

confidence: 99%

Cellular mechanisms of Δ⁹‐tetrahydrocannabinol behavioural sensitization

Rubino

Viganò

Massi

et al. 2003

Eur J of Neuroscience

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We investigated the cellular events linked to the induction of cannabinoid behavioural sensitization. In sensitized rats, autoradiographic binding studies with [3H]CP-55,940 showed a significant increase in cannabinoid receptor binding, specifically in the cerebellum, with no changes in the other brain areas where basal CB1-receptor expression is observed. In vitro autoradiography of CP-55,940-stimulated [35S]GTP gamma S binding provided a picture of cannabinoid receptor-mediated G protein activation. Basal [35S]GTP gamma S binding was not affected, whereas sensitized rats showed a significant increase of net [35S]GTP gamma S binding in the caudate putamen and cerebellum. Autoradiographic studies suggested that only these two areas had altered receptor functionality. We therefore focused our intracellular investigations only there, first surveying the responsiveness of the cAMP system to cannabinoids. CP-55,940 was unable to inhibit forskolin-induced cAMP accumulation in the cerebellum of sensitized animals, but no difference was observed between groups in the caudate putamen. Finally, we surveyed the levels of CREB and AP-1 binding activity, in the same two areas and found no difference in sensitized rats. The intracellular picture in sensitized rats suggests that besides the cAMP cascade, other signalling pathways may participate in the development of cannabinoid sensitization.

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“…These results suggest that c-Fos activation depends on specific neuronal connections and not on widespread, non-specific responses. In addition, the changes in c-fos expression were often seen following the first training session (Maleeva et al, 1989(Maleeva et al, , 1990Kaczmarek and Nikolajew, 1990;Nikolaev et al, 1992a, b;Lukasiuk et al, 1999;Anokhin et al, 2000;Bertaina-Anglade et al, 2000). Interestingly, in the reviewed literature an apparent controversy has emerged, on whether the elevated c-fos expression correlates with either learning or performing an already acquired task.…”

Section: Summary On C-fos Resultsmentioning

confidence: 99%

“…Using this paradigm, our group reported a marked increase in c-fos mRNA and protein levels in rat hippocampus, sensory and limbic cortex (Kaczmarek and Nikolajew, 1990;Lukasiuk et al, 1999;Nikolaev et al, 1992a, b). Later, in detailed analysis of c-Fos expression in the amygdala, we applied factor analysis to separate and group a number of behavioral components .…”

Section: Correlative Studies (C-fos Expression In Learning)mentioning

confidence: 99%

“…Levels of c-fos/c-Fos expression were found to be elevated in relevant brain structures in several further paradigms of avoidance learning as well as following other behavioral tasks (listed in Table 8.1). (Maleeva et al, 1989(Maleeva et al, , 1990Kaczmarek, 1990;Kaczmarek and Nikolajew, 1990;Tischmeyer et al, 1990;Nikolaev et al, 1992a, b;Lukasiuk et al, 1999;Savonenko et al, 1999;Radwanska et al, 2002) Other paradigms of avoidance learning…”

Section: Correlative Studies (C-fos Expression In Learning)mentioning

confidence: 99%

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