Tactile Experience Induces c-<i>fos</i> Expression in Rat Barrel Cortex

Filipkowski, Robert K.; Rydz, Marek; Berdel, B; Moryś, J; Kaczmarek, Leszek

doi:10.1101/lm.7.2.116

Cited by 76 publications

(52 citation statements)

References 43 publications

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“…Our findings in the somatosensory cortex, which were confirmed in the subset analysis, support the hypothesis that increased freezing and the concomitantly decreased exploratory behavior (as seen in ANX, as opposed to the exploring CTRL rats) led to hypometabolism in this part of the cortex, covering areas involved in exploration (e.g., barrel field) (Filipkowski et al, 2000). Although this cluster also comprised small portions of the left hippocampus, we assume that this is caused by a partial volume effect, resulting in blurring of the image due to limited spatial resolution.…”

Section: Discussionsupporting

confidence: 76%

Micro-Positron Emission Tomography Imaging of Rat Brain Metabolism during Expression of Contextual Conditioning

Luyten¹,

Casteels²,

Vansteenwegen³

et al. 2012

J. Neurosci.

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Using 18F-fluorodeoxygluose microPET imaging, we investigated the neurocircuitry of contextual anxiety versus control in awake, conditioned rats (n ϭ 7-10 per group). In addition, we imaged a group expressing cued fear. Simultaneous measurements of startle amplitude and freezing time were used to assess conditioning. To the best of our knowledge, no neuroimaging studies in conditioned rats have been conducted thus far, although visualizing and quantifying the metabolism of the intact brain in behaving animals is clearly of interest. In addition, more insight into the neurocircuitry involved in contextual anxiety may stimulate the development of new treatments for anxiety disorders. Our main finding was hypermetabolism in a cluster comprising the bed nucleus of the stria terminalis (BST) in rats expressing contextual anxiety compared with controls. Analysis of a subset of rats showing the best behavioral results (n ϭ 5 per subgroup) confirmed this finding. We also observed hypermetabolism in the same cluster in rats expressing contextual anxiety compared with rats expressing cued fear. Our results provide novel evidence for a role of the BST in the expression of contextual anxiety.

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Section: Discussionsupporting

confidence: 76%

Micro-Positron Emission Tomography Imaging of Rat Brain Metabolism during Expression of Contextual Conditioning

Luyten¹,

Casteels²,

Vansteenwegen³

et al. 2012

J. Neurosci.

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“…Notwithstanding the dominant labeling of pyramidal and spiny stellate cells (Filipkowski et al, 2000;Staiger et al, 2000Staiger et al, , 2002, our doubleimmunohistochemical data established that numerous recruited cells, located mainly in layers II/III, corresponded to COX-2 pyramidal cells. Consistent with the direct thalamic afferent input (Staiger et al, 1996b) of the overlapping populations of VIP-and ChAT-expressing GABA interneurons (Chédotal et al, 1994), we observed robust c-Fos induction in these cells throughout layers Figure 6.…”

Section: Identity Of the Cortical Neuronal Circuitry Recruited By Whimentioning

confidence: 88%

“…For anatomical studies, to prevent c-Fos induction related to stress and/or novelty (Staiger, 2006) rather than to the stimulus itself, rats were habituated to the stimulation protocol by a daily ϳ30 -45 min handling period for 1 week preceding the experimental day. During each session, rats were gently restrained in a plastic cone and the whiskers manually displaced with a paintbrush (3-4 Hz) (Filipkowski et al, 2000). Two days before the experiment, all but three whiskers (C1, C2, and C3) (Fig.…”

Section: Methodsmentioning

confidence: 99%

Pyramidal Neurons Are "Neurogenic Hubs" in the Neurovascular Coupling Response to Whisker Stimulation

Lecrux

Toussay

Kocharyan

et al. 2011

Journal of Neuroscience

150

165

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The whisker-to-barrel cortex is widely used to study neurovascular coupling, but the cellular basis that underlies the perfusion changes is still largely unknown. Here, we identified neurons recruited by whisker stimulation in the rat somatosensory cortex using double immunohistochemistry for c-Fos and markers of glutamatergic and GABAergic neurons, and investigated in vivo their contribution along with that of astrocytes in the evoked perfusion response. Whisker stimulation elicited cerebral blood flow (CBF) increases concomitantly with c-Fos upregulation in pyramidal cells that coexpressed cyclooxygenase-2 (COX-2) and GABA interneurons that coexpressed vasoactive intestinal polypeptide and/or choline acetyltransferase, but not somatostatin or parvalbumin. The evoked CBF response was decreased by blockade of NMDA (MK-801, Ϫ37%), group I metabotropic glutamate (MPEPϩLY367385, Ϫ40%), and GABA-A (picrotoxin, Ϫ31%) receptors, but not by GABA-B, VIP, or muscarinic receptor antagonism. Picrotoxin decreased stimulus-induced somatosensory evoked potentials and CBF responses. Combined blockade of GABA-A and NMDA receptors yielded an additive decreasing effect (Ϫ61%) of the evoked CBF compared with each antagonist alone, demonstrating cooperation of both excitatory and inhibitory systems in the hyperemic response. Blockade of prostanoid synthesis by inhibiting COX-2 (indomethacin, NS-398), expressed by ϳ40% of pyramidal cells but not by astrocytes, impaired the CBF response (Ϫ50%). The hyperemic response was also reduced (Ϫ40%) after inhibition of astroglial oxidative metabolism or epoxyeicosatrienoic acids synthesis. These results demonstrate that changes in pyramidal cell activity, sculpted by specific types of inhibitory GABA interneurons, drive the CBF response to whisker stimulation and, further, that metabolically active astrocytes are also required.

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“…Although strong c-Fos induction has been described 1-2 h after sensory stimulation (Filipkowski et al, 2000;Bisler et al, 2002) or fear conditioning (Herry and Mons, 2004), cortical c-Fos levels can rise as early as 30 min after sensory stimulation (Chaudhuri et al, 2000). We thus analyzed c-Fos induction 30 and 120 min after the beginning of sound pulse stimulation.…”

Section: Unpredictability Induces Sustained Amygdala Activation In Micementioning

confidence: 99%

Processing of Temporal Unpredictability in Human and Animal Amygdala

Herry¹,

Bach²,

Esposito³

et al. 2007

J. Neurosci.

358

291

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The amygdala has been studied extensively for its critical role in associative fear conditioning in animals and humans. Noxious stimuli, such as those used for fear conditioning, are most effective in eliciting behavioral responses and amygdala activation when experienced in an unpredictable manner. Here, we show, using a translational approach in mice and humans, that unpredictability per se without interaction with motivational information is sufficient to induce sustained neural activity in the amygdala and to elicit anxiety-like behavior. Exposing mice to mere temporal unpredictability within a time series of neutral sound pulses in an otherwise neutral sensory environment increased expression of the immediate-early gene c-fos and prevented rapid habituation of single neuron activity in the basolateral amygdala. At the behavioral level, unpredictable, but not predictable, auditory stimulation induced avoidance and anxietylike behavior. In humans, functional magnetic resonance imaging revealed that temporal unpredictably causes sustained neural activity in amygdala and anxiety-like behavior as quantified by enhanced attention toward emotional faces. Our findings show that unpredictability per se is an important feature of the sensory environment influencing habituation of neuronal activity in amygdala and emotional behavior and indicate that regulation of amygdala habituation represents an evolutionary-conserved mechanism for adapting behavior in anticipation of temporally unpredictable events.

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Tactile Experience Induces c-fos Expression in Rat Barrel Cortex

Cited by 76 publications

References 43 publications

Micro-Positron Emission Tomography Imaging of Rat Brain Metabolism during Expression of Contextual Conditioning

Micro-Positron Emission Tomography Imaging of Rat Brain Metabolism during Expression of Contextual Conditioning

Pyramidal Neurons Are "Neurogenic Hubs" in the Neurovascular Coupling Response to Whisker Stimulation

Processing of Temporal Unpredictability in Human and Animal Amygdala

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