Defective Central Immune Tolerance Induced by High-Dose D-Galactose Resembles Aging

Du, Hongmei; Wang, Y J; Liu, X; Wang, S L; Wu, Shengwen; Yuan, Zhe; Zhu, Xiaoying

doi:10.1134/s000629791906004x

Cited by 11 publications

(4 citation statements)

References 36 publications

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“…SOD is considered an anti-aging enzyme, and the level of natural SOD in the body decreases with age [16]. Studies showed that aging mice exhibited significant organ atrophy and reduced organ coefficient [17,18]. As shown in Figure 2, we found that SOD activity, lung weights, and lung organ coefficients of aging mice were significantly lower compared with the control group, which is consistent with previous reports.…”

Section: Discussionsupporting

confidence: 90%

Integrative Analysis of lncRNAs, miRNAs, and mRNA-Associated ceRNA Network in Lung Tissue of Aging Mice and Changes after Intervention of Codonopsis pilosula

Chen

Meng

et al. 2020

Med Sci Monit

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Background: Codonopsis pilosula is a traditional Chinese medicine that has an anti-aging effect. However, the anti-aging effect of Codonopsis pilosula on the lungs remains largely unknown, and the molecular mechanism also needs to be further studied. Thus, we investigated the protective effect of Codonopsis pilosula on the lungs of aging mice, and explored the underlying molecular mechanism. Material/Methods: We established an aging mouse model and then treated the mice with Codonopsis pilosula. Microarray analysis and bioinformatics methods were used to comprehensively analyze the lncRNA-miRNA-mRNA (ceRNA) network. Results: Our results showed that we successfully established the aging mouse model. The microarray analysis showed that 138 lncRNAs, 128 mRNAs, and 7 miRNAs were significantly changed after aging, and 282 lncRNAs, 283 mRNAs, and 19 miRNAs were dysregulated after treatment with Codonopsis pilosula. To explore the signaling pathways involved, KEGG pathway analysis was performed. Compared with the ceRNA network in aging mice and after treatment with Codonopsis pilosula, we found that 3 mRNAs (Hif3a, Zbtb16, Plxna2) and 1 lncRNA (NONMMUT063872) were associated with the anti-aging effect of Codonopsis pilosula and they were validated by quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Conclusions: Our results showed that Codonopsis pilosula has a protective effect on the aging lung, and the ceRNA network plays an important role in the anti-aging effect of Codonopsis pilosula.

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Section: Discussionsupporting

confidence: 90%

Integrative Analysis of lncRNAs, miRNAs, and mRNA-Associated ceRNA Network in Lung Tissue of Aging Mice and Changes after Intervention of Codonopsis pilosula

Chen

Meng

et al. 2020

Med Sci Monit

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“…D-gal-induced aging animal models have been extensively used to study aging mechanisms and anti-aging effects of drugs (27). Many studies show that the accumulation of dgal alters the antioxidant system and speeds up the process of aging (28). The previous studies showed that d-gal induced model could be a good choice for the study of age-dependent skeletal muscle degeneration (29,30).…”

Section: Introductionmentioning

confidence: 99%

Beneficial effects of whey protein peptides on muscle loss in aging mice models

Zhu

et al. 2022

Front. Nutr.

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Aging-related muscle loss is a hallmark of aging and is the cause of some negative outcomes. An optimized diet and supplements have a positive effect in slowing down the process of muscle loss. D-galactose(d-gal) has been used widely to develop aging model. This study explored the beneficial effects of whey protein peptides (WPPs) on sarcopenia in d-gal-induced aging mice. A total of 72 SPF male C57BL/6N mice were used in this study. Sixty mice were modeled by injected intraperitoneally with d-gal (100 mg/kg body weight for 6 weeks), and the other 12 mice were used as control, and injected with the same amount of normal saline. After 6 weeks, the modeled mice were randomly divided into the model control group, whey protein group (1.5 g/kg*bw), and three WPPs intervention groups (0.3 g/kg*bw, 1.5 g/kg*bw, 3.0 g/kg*bw), according to serum malondialdehyde (MDA) level. The test samples were orally given to mice by daily garaged. During the 30 days intervention period, the model control group, whey protein group, and WPPs group continued receiving intraperitoneal injections of d-gal, whereas the control group continued receiving intraperitoneal injections of normal saline. The results showed that WPPs could significantly improve the grip strength of aged mice. WPPs could significantly increase lean mass of aged mice and increase muscle weight of gastrocnemius and extensor digitorum longus. WPPs could significantly increase the level of insulin-like growth factor-1 (IGF-1) and reduce level of interleukin (IL)-1, IL-6, tumor necrosis factor-alpha (TNF-α) in serum. WPPs could affect the muscle fiber size in d-gal-induced aging mice. Its specific mechanism may be related to the activation of IGF-1/Akt/mTOR protein synthesis signaling pathway and reduction of the level of inflammation. These results indicate that WPPs can improve aging-related sarcopenia. Compared with whey protein, WPPs supplement seems a better form for sarcopenia.

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“…continuous modelling was performed for 42 days. For the preparation of the model of aging, a decrease in body weight loss and thymus index indicated the successful establishment of the model (25)(26)(27).…”

Section: Animalsmentioning

confidence: 99%

Integration of lncRNA and mRNA profiles to reveal the protective effects of Codonopsis pilosula extract on the gastrointestinal tract of mice subjected to D‑galactose‑induced aging

Meng

Chen

et al. 2020

Int J Mol Med

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Codonopsis pilosula is a type of traditional chinese medicine that exerts an anti-aging effect and can regulate the gastrointestinal (GI) system. The aim of the present study was to investigate the underlying molecular mechanisms responsible for the anti-aging effects of Codonopsis pilosula in the GI tract of mice with d-galactose-induced aging. First, a successful mouse model of aging was established, and Codonopsis pilosula water extract was then used for treatment. The anti-aging effects of Codonopsis pilosula on the GI tract were then detected from the perspectives of tissue structure, physiological function and cell ultrastructure. Finally, in order to explore the underlying molecular mechanisms, the expression profiles of lncRNAs and mRNAs in the stomach and intestine were examined using microarray technology. A total of 117 (41 lncRNAs and 76 mRNAs) and 168 (85 lncRNA sand 83 mRNAs) differentially expressed genes associated with the anti-aging effects of Codonopsis pilosula were identified in the stomach and intestine, respectively. Through integrated analysis of the stomach and intestine, 4 hub RNAs, including 1 lncRNA (LOc105243318) and 3 mRNAs (Fam132a, Rorc and 1200016E24Rik) were identified, which may be associated with the anti-aging effects of Codonopsis pilosula in the GI tract of aging mice. The Kyoto Encyclopedia of Genes and Genomes analysis revealed that the metabolic pathway was an important pathway underlying the anti-aging effects of Codonopsis pilosula in the GI tract. On the whole, in the present study, 4 hub RNAs associated with these effects and their regulatory networks were found in the GI tract of aging mice. In addition, the metabolic pathway was found to play an important role in these anti-aging effects in the GI tract.

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Defective Central Immune Tolerance Induced by High-Dose D-Galactose Resembles Aging

Cited by 11 publications

References 36 publications

Integrative Analysis of lncRNAs, miRNAs, and mRNA-Associated ceRNA Network in Lung Tissue of Aging Mice and Changes after Intervention of Codonopsis pilosula

Integrative Analysis of lncRNAs, miRNAs, and mRNA-Associated ceRNA Network in Lung Tissue of Aging Mice and Changes after Intervention of Codonopsis pilosula

Beneficial effects of whey protein peptides on muscle loss in aging mice models

Integration of lncRNA and mRNA profiles to reveal the protective effects of Codonopsis pilosula extract on the gastrointestinal tract of mice subjected to D‑galactose‑induced aging

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