Deep Proteomic Insights into the Individual Short‐Term Pellicle Formation on Enamel—An In Situ Pilot Study

Trautmann, Simone; Künzel, Nicolas; Fecher‐Trost, Claudia; Barghash, Ahmad; Schalkowsky, Pascal; Dudek, Johanna; Delius, Judith; Helms, Volkhard; Hannig, Matthias

doi:10.1002/prca.202070054

Cited by 15 publications

(21 citation statements)

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“…In a recent in situ study, 498 proteins were identified in individual pellicles samples formed on bovine enamel. The higher number of identified proteins in that study is likely to be due to the much bigger surface area (8 cm 2 ) sampled and the fact that the pellicle is collected using solutions that cannot be employed in the clinical condition (25). The total number of proteins identified in situ was virtually identical to the one in vivo (170 proteins), which is lower than previously reported by V entura and coworkers (10).…”

Section: Discussionmentioning

confidence: 98%

Proteomic profiles of the acquired enamel pellicle formed in vitro, in situ, or in vivo

Pelá

Lunardelli

Ventura

et al. 2020

European J Oral Sciences

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This study compared the protein profile of the acquired enamel pellicle (AEP) formed under three conditions: in vitro, in situ, and in vivo. Nine volunteers participated in all procedures. In the in vitro condition, the volunteers donated saliva, in which specimens were incubated to form the AEP. In the in situ condition, the volunteers used an oral device containing specimens where the AEP was formed. In the in vivo condition, the AEP was collected from the volunteers own teeth. All AEPs were formed for 120 min, collected and processed by mass spectrometry. Overall, a total of 321 proteins were identified, among which 37 proteins are commonly considered typical in the AEP. For each of the in vitro, in situ, and in vivo conditions, respectively, 66, 174, and 170 proteins were identified. For the in vitro condition, 17 pellicle-typical proteins were not identified. Furthermore, several proteins with important functions within the AEP presented differences in expression in the three conditions. The qualitative profile of the proteins, especially the typical ones, is different in the in vitro condition. In addition, there are important quantitative differences that may interfere when attempting to extrapolate in vitro results to an in situ and in vivo condition.

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Section: Discussionmentioning

confidence: 98%

Proteomic profiles of the acquired enamel pellicle formed in vitro, in situ, or in vivo

Pelá

Lunardelli

Ventura

et al. 2020

European J Oral Sciences

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“…The formation of the mucosal pellicle is influenced by hydrophobic interactions and the cross-linking of proteins and salivary components [ 125 , 126 , 127 , 128 , 129 ]. Microorganisms can directly adhere to the epithelial cell membranes, whereas on the tooth surface, bacteria adhere indirectly through attachment to the acquired enamel pellicle layer [ 124 , 130 , 131 , 132 , 133 ]. The mucosal pellicle’s components are represented by soluble and epithelial mucins (mucin 5B, 7, 1, 4, 16) CA VI, sIgA, cystatin, amylase and PrPs [ 125 , 129 , 134 , 135 , 136 ].…”

Section: Pellicle Formation and Initial Bacterial Colonization On Solid Surfacesmentioning

confidence: 99%

Preventive Applications of Polyphenols in Dentistry—A Review

Flemming

Meyer-Probst

Speer

et al. 2021

IJMS

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Polyphenols are natural substances that have been shown to provide various health benefits. Antioxidant, anti-inflammatory, and anti-carcinogenic effects have been described. At the same time, they inhibit the actions of bacteria, viruses, and fungi. Thus, studies have also examined their effects within the oral cavity. This review provides an overview on the different polyphenols, and their structure and interactions with the tooth surface and the pellicle. In particular, the effects of various tea polyphenols on bioadhesion and erosion have been reviewed. The current research confirms that polyphenols can reduce the growth of cariogenic bacteria. Furthermore, they can decrease the adherence of bacteria to the tooth surface and improve the erosion-protective properties of the acquired enamel pellicle. Tea polyphenols, especially, have the potential to contribute to an oral health-related diet. However, in vitro studies have mainly been conducted. In situ studies and clinical studies need to be extended and supplemented in order to significantly contribute to additive prevention measures in caries prophylaxis.

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“…Then the samples were either directly treated with elution buffer to elute the adsorbed macromolecules or were incubated in PBS at room temperature and the elution was performed 3.5 h later. The elution, subsequent precipitation and final preparation for SDS-PAGE and Coomassie-staining were conducted [41].…”

Section: Sample Characterizationmentioning

confidence: 99%

“…To determine the molecular weight of the macromolecules of the conditioning films we performed sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Coomassie-staining as previously described by Trautmann et al at two different ages of the films [41]. The specimens consisting exclusively of enamel with a total surface area of 8 cm 2 were purified with 3 % NaOCl, washed with water, ultrasonicated in 70 % isopropanol, and air-dried before exposure to either saliva or PBS for 3 min at room temperature.…”

Section: Sample Characterizationmentioning

confidence: 99%

“…For example, it has been shown that a salivary macromolecule film, termed pellicle, forms within seconds after a surface is brought into contact with saliva [39]. The pellicle reaches a thickness of around 7 nm within three minutes and is free from bacteria at this early stage [40,41]. We also incubate bacteria in saliva to mimic the natural case, in which a bacterium comes into contact with the surfaces we chose from the oral cavity setting.…”

Section: Introductionmentioning

confidence: 99%

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Hydroxyapatite pellets as versatile model surfaces for systematic studies on enamel

Mischo

Faidt

McMillan

et al. 2021

Preprint

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Research into materials for medical application draws inspiration from naturally occurring or synthesized surfaces, just like many other research directions. For medical application of materials, particular attention has to be paid to biocompatibility, osseointegration and bacterial adhesion behavior. To understand their properties and behavior, experimental studies with natural materials such as teeth are strongly required. The results, however, may be highly case-dependent because natural surfaces have the disadvantage of being subject to wide variations, for instance in their chemical composition, structure, morphology, roughness, and porosity. A synthetic surface which mimics enamel in its performance with respect to bacterial adhesion and biocompatibility would, therefore, facilitate systematic studies much better. In this study, we discuss the possibility of using hydroxyapatite (HAp) pellets to simulate the surfaces of teeth and show the possibility and limitations of using a model surface. We performed single-cell force spectroscopy with single Staphylococcus aureus cells to measure adhesion-related parameters such as adhesion force and rupture length of adhesins binding to HAp and enamel. We also examine the influence of blood plasma and saliva on the adhesion properties of S. aureus. The results of these measurements are matched to water wettability, elemental composition of the samples and the change in the macromolecules adsorbed over time. We found that the adhesion properties of S. aureus were similar on both samples under all conditions: Significant decreases in adhesion strength were found equally in the presence of saliva or blood plasma on both surfaces. We therefore conclude that HAp pellets are a good alternative for natural dental material. This is especially true when slight variations in the physicochemical properties of the natural materials may affect the experimental series.

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Deep Proteomic Insights into the Individual Short‐Term Pellicle Formation on Enamel—An In Situ Pilot Study

Cited by 15 publications

References 0 publications

Proteomic profiles of the acquired enamel pellicle formed in vitro, in situ, or in vivo

Proteomic profiles of the acquired enamel pellicle formed in vitro, in situ, or in vivo

Preventive Applications of Polyphenols in Dentistry—A Review

Hydroxyapatite pellets as versatile model surfaces for systematic studies on enamel

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