Deep divergence and hybridization among sympatric Neotropical toads

Vallinoto, Marcelo; Cunha, Divino Bruno da; Bessa-Silva, Adam; Sodré, Davidson; Sequeira, Fernando

doi:10.1093/zoolinnean/zlw001

Cited by 8 publications

(4 citation statements)

References 76 publications

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“…Finally, on the basis of EWL rates, we estimated skin resistance (R s ) to water evaporation and provide an evaluation of the potential error in estimates of skin permeability assuming EWL Resp as negligible. We chose R. diptycha for the present study because this species has a terrestrial lifestyle and is broadly distributed in tropical biomes of South America (Haddad et al, 2013;Vallinoto et al, 2017) and, therefore, it is likely to experience natural fluctuations in body temperature and hydration state.…”

Section: Introductionmentioning

confidence: 99%

Temperature and dehydration effects on metabolism, water uptake, and the partitioning between respiratory and cutaneous evaporative water loss in a terrestrial toad

Senzano

Andrade

2018

Journal of Experimental Biology

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Terrestrial anurans often experience fluctuations in body temperature and hydration state, which are known to influence evaporative water loss through the skin (EWL Skin ) and lungs (EWL Resp ). These effects arise from associated changes in skin permeability, metabolism and lung ventilation. Herein, we determined the rates of EWL Skin and EWL Resp in the terrestrial toad Rhinella diptycha at different temperatures and hydration states. We measured oxygen uptake rates to verify whether alterations in the partitioning between EWL Skin and EWL Resp were associated with metabolism-induced changes in pulmonary gas exchange. We also measured the influence of hydration and temperature on water uptake (WU) through the skin. Finally, as estimates of skin resistance to evaporation (R s ) are usually inferred from total evaporative water loss (EWL Total ), under the assumption of negligible EWL Resp , we calculated the potential error in accepting this assumption for different temperature and hydration states. EWL Skin and EWL Resp increased with temperature, but this response was greater for EWL Resp , which was attributed to the temperature-induced elevation in metabolism and lung ventilation. Dehydration caused a decrease in the relative contribution of EWL Skin to EWL Total , mirrored by the concurrent increase in the contribution of EWL Resp , at all temperatures. Thus, R s increased with dehydration. WU rates were dictated by dehydration with little influence of temperature. The partitioning between EWL Skin and EWL Resp was affected by both temperature and hydration state and, under some conditions, considering EWL Resp as negligible led to significant errors in the assessment of skin resistance to evaporation.

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Section: Introductionmentioning

confidence: 99%

Temperature and dehydration effects on metabolism, water uptake, and the partitioning between respiratory and cutaneous evaporative water loss in a terrestrial toad

Senzano

Andrade

2018

Journal of Experimental Biology

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“…Hybridization is considered to be an important driving force for the adaptive evolution of species [ 22 , 23 ]. Introgression caused by hybridization has been reported in many animals, such as insects [ 24 ], mollusks [ 25 ], mammals [ 26 ], reptiles [ 27 ], amphibians [ 28 ], and fish [ 29 ]. In recent decades, with the frequent occurrence of invasive species introduction events, hybridization opportunities between invasive species and their relatives have increased, and introgressive hybridization progeny may be more invasive, leading to the extinction of local parent groups [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of the invasive gall pest Leptocybe invasa (Hymenoptera: Apodemidae) from China

et al. 2021

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Leptocybe invasa Fisher et LaSalle is a global invasive pest that seriously damages Eucalyptus plants. Studying the genetic diversity, genetic structure and introgression hybridization of L. invasa in China is of great significance for clarifying the breeding strategy, future invasion and diffusion trends of L. invasa in China and developing scientific prevention and control measures. Genetic diversity and phylogenetic analyses of 320 L. invasa female adults from 14 geographic populations in China were conducted using 10 polymorphic microsatellite loci (SSRs) and mitochondrial DNA cytochrome oxidase I gene sequences (COIs). (1) The Bayesian phylogenetic tree and haplotype network diagram showed that only haplotype Hap3 existed in L. invasa lineage B in China, while haplotypes Hap1 and Hap2 existed in lineage A, among which haplotype Hap2 was found for the first time. The nucleotide and haplotype diversities of lineage A were higher than those of lineage B. (2) The SSR genetic diversity of the Wuzhou Guangxi, Ganzhou Jiangxi and Panzhihua Sichuan populations was higher than that of the other 11 populations, and the SSR genetic diversity of lineage A was higher than that of lineage B. (3) The AMOVA analysis of mitochondrial COI data showed that 75.55% of the variation was among populations, and 99.86% of the variation was between lineages, while the AMOVA analysis of nuclear SSR data showed that 35.26% of the variation was among populations, and 47.04% of the variation was between lineages. There were obvious differences in the sources of variation between the COI and SSR data. (4) The optimal K value of COI and SSR data in structure analysis was 2, and PCoA analysis also divided the dataset into two obvious categories. The UPMGA phylogenetic tree based on SSR data clustered 14 geographic species into two groups. The results of genetic structure analysis supported the existence of two lineages, A and B, in China. (5) Structural analysis showed that there was obvious introgressive hybridization in Wuzhou Guangxi, Ganzhou Jiangxi, Panzhihua Sichuan and other populations. These results suggest that lineage introgressive hybridization has occurred in the L. invasa population in China. The introgressive hybridization degree and genetic diversity of lineage A are obviously higher than those of lineage B. Lineage introgressive hybridization may be the driving force for further L. invasa invasion and diffusion in China in the future.

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“…Hybridization is considered an important driver of adaptive evolution 1,2 . Hybridization and gene introgression have been reported in many animals, including mollusks, 3 insects, 4 fishes, 5 amphibians, 6 reptiles, 7 and mammals 8 . Aquatic fauna, with the absence of dispersal barriers preventing their contact, are especially prone to produce hybrids among closely related species 9 .…”

Section: Introductionmentioning

confidence: 99%

Introgressive hybridization between two non‐native apple snails in China: widespread hybridization and homogenization in egg morphology

Yang

Liu

et al. 2020

Pest Management Science

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BACKGROUND: Apple snails from the genus Pomacea have spread widely in paddy fields and other wetlands of southern China since their introduction in the 1980s. Pomacea spp. are commonly identified using mitochondrial COI sequences. However, sequencing the nuclear elongation factor 1-alpha (EF1⊍) gene revealed genetic introgression between field populations of P. canaliculata and P. maculata, which produce surviving hybrids in laboratory crossbreeding experiments. RESULTS: In this study, we sequenced 1054 EF1⊍ clones to design specific primers and established a fast and accurate multiplex polymerase chain reaction (PCR) method for genotyping EF1⊍. Combined with genotyping P. canaliculata and P. maculata based on mitochondrial COI and nuclear EF1⊍, we revealed the genetic introgression patterns of 30 apple snail populations in China. Purebred and hybrid individuals of P. canaliculata were widely distributed, while pure maculata-EF1⊍ type was detected only in a few individuals identified as P. canaliculata based on COI sequences. Each egg clutch had one to three genetic patterns, indicating multiple paternity or segregation in the progeny of hybrids. The higher percentages of hybrids in both wild populations and progeny than the homozygotes indicated a potential heterosis in the apple snail populations. Additionally, egg size and clutch size of the apple snails became homogeneous among the non-native populations exhibiting introgression hybridization. CONCLUSION: Our findings emphasize the value of apple snails as a model to study the mechanisms and impacts of introgressive hybridization on fitness traits.

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Deep divergence and hybridization among sympatric Neotropical toads

Cited by 8 publications

References 76 publications

Temperature and dehydration effects on metabolism, water uptake, and the partitioning between respiratory and cutaneous evaporative water loss in a terrestrial toad

Temperature and dehydration effects on metabolism, water uptake, and the partitioning between respiratory and cutaneous evaporative water loss in a terrestrial toad

Genetic diversity analysis of the invasive gall pest Leptocybe invasa (Hymenoptera: Apodemidae) from China

Introgressive hybridization between two non‐native apple snails in China: widespread hybridization and homogenization in egg morphology

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