Deep and Superficial Keloid Fibroblasts Contribute Differentially to Tissue Phenotype in a Novel In Vivo Model of Keloid Scar

Abstract

Aspects of the phenotypes of engineered skin substitutes prepared with keloid cells are analogous to thickening and spreading of human keloid scars. Therefore, use of keloid engineered skin substitutes is a valuable new tool for the study of keloid scarring.

Abdominal aortic aneurysm and histological, clinical, radiological correlation

“…The same products of collagen degradation subsequently retrieve additional immune cells. Despite the fact that the collagen is degraded, fibroblasts and smooth muscle cells increase local production of type I collagen produced above all during inflammation (Supp et al, 2012). Type I collagen are more resistant and less elastic fibres, able to confer greater rigidity than type III collagen.…”

Reconstitution of Human Keloids in Mouse Skin

“…13 However, xenografts contain not only cellular components but also donor-derived extracellular matrix, which makes it difficult to be certain that it is the keloid-derived cells that are responsible for any observed keloid phenotype. A related approach was used by Supp et al 6 , who grafted engineered human skin substitutes with keloid-derived fibroblasts and keratinocytes to athymic mice, and the resulting grafts were thicker and wider than grafts made with normal skin-derived cells. However, their organotypic skin included an artificial collagen–glycosaminoglycan scaffold and did not have macroscopic or pathological features of keloids.…”

Keloids: The paradigm of skin fibrosis — Pathomechanisms and treatment

“…In a recent study, the location of fibroblasts within the lesional skin also appears to contribute differentially to the fibrosis. Isolated deep fibroblasts display elevated expressions of COL1A1, TGF-β1, periostin, PAI-2, and inhibin beta A compared to their superficial and normal counterparts [19]. …”