Abdominal aortic aneurysm and histological, clinical, radiological correlation

Rodella, Luigi Fabrizio; Rezzani, Rita; Bonomini, Francesca; Peroni, Michele; Cocchi, Marco Angelo; Hirtler, Lena; Bonardelli, Stefano

doi:10.1016/j.acthis.2016.01.007

Cited by 22 publications

(16 citation statements)

References 49 publications

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“…Previous studies have indicated that cd34 cells are closely related to the development of aneurysms. Patients with AAA have a higher proportion of cd34 + cells than patients with peripheral vascular disease (PVd) (55,56). However, in our study, cd34 + cells were difficult to find.…”

Section: Discussioncontrasting

confidence: 64%

The active participation of p22phox-214T/C in the formation of intracranial aneurysm and the suppressive potential of edaravone

Luo

Wang

et al. 2018

Int J Mol Med

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Oxidative stress reactions play an important role in the pathogenesis of intracranial aneurysm (IA). p22phox is involved in the oxidative stress reaction, and it is a critical subunit of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. The present study investigated the association of genetic variants within the gene encoding p22phox‑214T/C with IA. The p22phox‑214T/C gene polymorphisms in 192 cases of IA and 112 controls were analyzed by polymerase chain reaction‑restriction fragment length polymorphism (PCR‑RFLP). The mRNA expression of NADPH oxidase was also analyzed by RT‑PCR. The results of RT‑PCR were validated by ELISA. In a rabbit model of elastase‑induced aneurysm, we used edaravone for anti‑oxidative stress treatment to observe the curative effects. In the clinical cases, a significant difference in p22phox‑214T/C allele frequencies in the IA group was observed compared with the control group (P<0.001). The expression level of NADPH oxidase was differed significantly between the IA group and the control group. In the rabbit model of elastase‑induced aneurysm, the success rate of the aneurysmal model in the edaravone group and the wound ulcer rate were lower than those in the control group. In addition, the diameter of the aneurysm was smaller than in the edaravone group than in the control group (3.26±0.13 mm vs. 3.85±0.07 mm), and the expression of matrix metalloproteinase‑9 (MMP‑9) was significantly lower than that in the control group (P<0.0001). Thus, these data suggest the active participation of p22phox‑214T/C in the formation of IA and the suppressive potential of edaravone against IA formation.

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Section: Discussioncontrasting

confidence: 64%

The active participation of p22phox-214T/C in the formation of intracranial aneurysm and the suppressive potential of edaravone

Luo

Wang

et al. 2018

Int J Mol Med

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“…A study of human infra-renal aortic samples demonstrated that aneurysmal tissue exhibits an increase in SMC apoptosis and a decrease in SMC density (Rowe et al, 2000). In addition, MMP-induced degradation of elastin contributes to AAA pathogenesis (Kadoglou & Liapis, 2004), and the diseased aorta has altered collagen levels with an increase in type I and a decrease in type III (Rodella et al, 2016). Mutations in collagen and fibrillin-1, a key protein in elastic fiber-associated microfibrils, as in Ehlers-Danlos and Marfan syndromes, respectively, predispose to aortic aneurysm, dissection and rupture.…”

Section: Cardiovascular Diseasesmentioning

confidence: 99%

Vascular Cells in Blood Vessel Wall Development and Disease

Mazurek

Dave

Chandran

et al. 2017

Advances in Pharmacology

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The vessel wall is composed of distinct cellular layers, yet communication among individual cells within and between layers results in a dynamic and versatile structure. The morphogenesis of the normal vascular wall involves a highly regulated process of cell proliferation, migration and differentiation. The use of modern developmental biological and genetic approaches has markedly enriched our understanding of the molecular and cellular mechanisms underlying these developmental events. Additionally, the application of similar approaches to study diverse vascular diseases has resulted in paradigm-shifting insights into pathogenesis. Further investigations into the biology of vascular cells in development and disease promise to have major ramifications on therapeutic strategies to combat pathologies of the vasculature.

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“…CD34, CD68, and Ki67 were detected in both human aneurysm and elastase-induced fusiform aneurysms, but not in control arteries (Figures 8, 9, and 10). CD34 is an important proinflammatory and proangiogenic cell in chronic inflammatory vascular disease [13]. The aneurysm tissues have some CD34-positive cell infiltration, while the control arteries have no significant CD34 positive cells, which prove that the characteristics of modeling aneurysms are consistent with those of normal aneurysms (Figure 8).…”

Section: Resultsmentioning

confidence: 67%

An Effective and Simple Way to Establish Elastase-Induced Middle Carotid Artery Fusiform Aneurysms in Rabbits

Lyu

Luo

Zhang

et al. 2020

BioMed Research International

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Objective. Elastase-induced aneurysms in rabbits have been proposed as a preclinical tool for device development, but there is still much deficiency in those aneurismal models. So we need to explore the efficient and convenient animal models for the investigation of intracranial aneurysms. Then, we compared and analyzed three methods of elastase-induced carotid artery aneurysms in rabbits and aimed to find a simple, effective, and reproducible method for creating elastase-induced aneurysms. Methods. 42 standard feeding male adult Japanese white rabbits (3.05±0.65 kg) were randomly divided into 3 groups and treated with elastase ablation to create common carotid artery (RCCA) aneurysm models: Group A (root-RCCA medication group, n=12), Group B (mid-RCCA medication group, n=18), and Group C (ligated RCCA+medication group, n=12). For Group A, the origin of the RCCA was blocked by two temporary aneurysm clips, and the resulting 2 cm cavity was infused with elastase for 20 min, then the clip was removed and the RCCA was not ligated. For Group B, the middle part of RCCA was treated the same way as Group A and the RCCA was not ligated. For Group C, the middle part of RCCA was treated as Group B, but the distal RCCA was ligated. After the aneurysm models were created for 3 weeks, prior to sacrificing the animals, color Doppler ultrasound and angiography were performed for blood flow measurements inside the aneurysms. Histological analysis (such as SMA-α, CD31, CD34, CD68, collagen IV, and Ki67) and the other relevant indexes were compared between the ideal model’s aneurysmal tissues and the human intracranial aneurysm’s tissues to confirm whether we have successfully established elastase-induced aneurysm models. Results. Compared with human intracranial aneurysm specimens by the color Doppler ultrasound, angiography, and changes in the inner diameter of arteries, all three methods have successfully established the elastase-induced aneurysm models. Histology showed that biological responses were similar to both human cerebral aneurysms and previously published elastase-induced rabbit aneurysm models. Group A and Group B had the same morphology, but Group A had a higher mortality rate than Group B. Group B and Group C had different morphology. The aneurysm of Group C was more similar to human cerebral aneurysms but had a higher mortality rate than Group B. Group B was confirmed not only as an alternative method but also as a more safe and effective method for creating elastase-induced aneurysm models. Conclusion. Through analysis and comparison, the Group B is proven to be the simplest, reproducible, and most effective modeling method. The aneurysm model established by Group B can be used for basic research related to aneurysm mechanism. We have provided a new and effective method for basic research on aneurysm.

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Abdominal aortic aneurysm and histological, clinical, radiological correlation

Cited by 22 publications

References 49 publications

The active participation of p22phox-214T/C in the formation of intracranial aneurysm and the suppressive potential of edaravone

The active participation of p22phox-214T/C in the formation of intracranial aneurysm and the suppressive potential of edaravone

Vascular Cells in Blood Vessel Wall Development and Disease

An Effective and Simple Way to Establish Elastase-Induced Middle Carotid Artery Fusiform Aneurysms in Rabbits

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