Decreased thrombin activity by a Congolese herbal medicine used in sickle cell anemia

Nsimba, Marie Miezi; Lami, José Nzunzu; Hayakawa, Yumiko; Yamamoto, Chika; Kaji, Toshiyuki

doi:10.1016/j.jep.2013.05.038

Cited by 7 publications

(4 citation statements)

References 43 publications

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“…Thrombin activity was measured by aqueous extracts from Ceiba pentandra (Malvaceae/Bombacoideae) and Quassia africana (Simaroubaceae) ( C. pentandra ). The results indicated the reduction of thrombin activity and prolonged plasma clotting time through affecting coagulation of intrinsic pathway [ 91 ]. Although there is no direct evidence that herbal medicines target thrombin in degenerative arthritis, the above results indicate both reduction of thrombin activity and extension of plasma clotting time, thus affecting the intrinsic coagulation pathway.…”

Section: Thrombin As a Therapeutic Target In Oamentioning

confidence: 99%

The Characteristics of Thrombin in Osteoarthritic Pathogenesis and Treatment

Chou

Huang

et al. 2014

BioMed Research International

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Osteoarthritis (OA) is a mechanical abnormality associated with degradation of joints. It is characterized by chronic, progressive degeneration of articular cartilage, abnormalities of bone, and synovial change. The most common symptom of OA is local inflammation resulting from exogenous stress or endogenous abnormal cytokines. Additionally, OA is associated with local and/or systemic activation of coagulation and anticoagulation pathways. Thrombin plays an important role in the stimulation of fibrin deposition and the proinflammatory processes in OA. Thrombin mediates hemostatic and inflammatory responses and guides the immune response to tissue damage. Thrombin activates intracellular signaling pathways by interacting with transmembrane domain G protein coupled receptors (GPCRs), known as protease-activated receptors (PARs). In pathogenic mechanisms, PARs have been implicated in the development of acute and chronic inflammatory responses in OA. Therefore, discovery of thrombin signaling pathways would help us to understand the mechanism of OA pathogenesis and lead us to develop therapeutic drugs in the future.

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Section: Thrombin As a Therapeutic Target In Oamentioning

confidence: 99%

The Characteristics of Thrombin in Osteoarthritic Pathogenesis and Treatment

Chou

Huang

et al. 2014

BioMed Research International

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“…The hydroethanolic extract of L. furfuracea prolonged PT, APTT and TT in a concentration-dependent, unlike other plants with anticoagulant activity [58][59][60][61] L. furfuracea prolongs the three times of clotting. In Echinacea purpurea and Fragaria vesca, the coagulation times were prolonged to more than 600 s for APTT and more than 300 s for PT [61]; meanwhile, the Crataegus monogyna extract prolonged clotting times from 37.9 to 350.5 s for APTT and from 13.4 to 16.5 s for PT [58].…”

Section: Effect Of Hydroethanolic Extract On Clotting Timesmentioning

confidence: 92%

Laelia furfuracea Lindl.: an Endemic Mexican Orchid with Anticoagulant Activity

et al. 2021

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Abstract. Laelia furfuracea is an endemic orchid from Mexico, with antioxidant activity. The objective of this study was to evaluate the effect of hydroethanolic extract and fractions obtained from the orchid leaves on the clotting times of patients with venous thromboembolic disease (VTD) and to identify their tentative compounds. The anticoagulant activity was evaluated by determining prothrombin (PT), thrombin (TT) and, activated partial thromboplastin (APTT) times. Identification of the compounds was carried out using a chromatographic technique with an ultra-high-performance liquid chromatographic analyzer coupled with electrospray ionization with quadrupole time of flight-mass-mass spectrometry. The extract prolonged the clotting times depending on the concentration-response (5-60 mg / mL); 25 mg/mL prolonged the PT (33.2 ± 2.3 s) and TT (33.1 ± 0.3 s); and APTT (61.8 ± 3.4 s) at a concentration of 15 mg/mL. The main groups tentatively identified were xanthine, carboxylic acid, amino acid, and phenolic compounds. These compounds or the synergy between them prolong clotting times. Laelia furfuracea is an orchid with research potential in the search for new anticoagulant agents. Resumen. Laelia furfuracea es una orquídea endémica de México, la cual posee actividad antioxidante. El objetivo de este estudio fue evaluar el efecto del extracto hidroetanólico y fracciones obtenidas de hojas de la orquídea sobre los tiempos de coagulación de pacientes con enfermedad tromboembólica venosa (ETV) e identificar sus posibles compuestos. La actividad anticoagulante se evaluó determinando los tiempos de protrombina (TP), trombina (TT) y tromboplastina parcial activada (TTPA). La identificación de los compuestos se realizó usando una técnica cromatográfica con un analizador cromatográfico líquido de Ultra Alta Resolución con Ionización por electroespray acoplado a espectrometría de masas con Cuadrupolo y Tiempo de Vuelo. El extracto prolongó los tiempos de coagulación dependiente de la concentración-respuesta (5-60 mg/mL); 25 mg/mL prolongó el TP (33.2±2.3 s) y TT (33.1±0.3 s); y TTPA (61.8±3.4 s) a una concentración de 15 mg/mL. Los principales grupos de posibles compuestos identificados fueron xantina, ácido carboxílico, aminoácido y compuestos fenólicos. Estos compuestos o la sinergia entre ellos prolongan los tiempos de coagulación. Laelia furfuracea es una orquídea con potencial en investigación para la búsqueda de nuevos agentes anticoagulantes.

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“…Using EDTA as a positive control, the colour reduction is used to gauge the compound's iron chelating action. 26 Serial dilutions (11) of 0.1 mM EDTA stock solution in water were created at concentrations of 5,10,15,20,25,30,35,40,50,60, and 70 mM. Samples of enriched extracts from red and white owers were prepared at a concentration of 0.625 mg mL −1 in methanol.…”

Section: Liquid Chromatography-mass Spectrometry (Lc-ms) Prolingmentioning

confidence: 99%

“…14 Interestingly, Ceiba pentandra (L.) Gaertn ( Bombacaceae ) is used traditionally in Congo to treat SCA and it's aqueous bark extract exhibited significant antisickling and antithrombing activities. 15 Also, previous reports attributed the antisickling effect of several plants to their high anthocyanin content, present mainly in the flowers. 16,17 As little data was available concerning P. ellipticum flowers phytochemical makeup especially anthocyanins, and further prompted by the well reported anthocyanins antisickling potential, this study aims to identify phytoconstituents especially anthocyanins of potential effects in SCA using LC/MS.…”

Section: Introductionmentioning

confidence: 99%