Decreased RNA Polymerase Activity in Mammalian Zinc Deficiency

Terhune, M. W.; Sandstead, Harold H.

doi:10.1126/science.177.4043.68

Cited by 111 publications

(24 citation statements)

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“…As a micro-element, manganese acts either as a cofactor activating a number of enzymes thereby forming metal-enzyme complexes or as an integral part of some metalloenzymes [12], while zinc has been reported to increase the activities of more than 70 enzymes [13]. Moreover, a retarded DNA, RNA and protein synthesis and subsequent impairment in cellular division, growth and repair of worn-out tissues has also been reported in zinc deficient animals [14]. Amongst the eighteen amino acids observed in this mushroom, glutamic acid and aspartic acid were in fairly high concentrations.…”

Section: Resultsmentioning

confidence: 99%

Nutriceutical potential of Pleurotus tuber-regium sclerotium

Ohiri¹

2018

Ukr.Biochem.J

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Section: Resultsmentioning

confidence: 99%

Nutriceutical potential of Pleurotus tuber-regium sclerotium

Ohiri¹

2018

Ukr.Biochem.J

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“…A normal developmental pattern of RNA concentration (milligrams per gram wet weight) was maintained in all three test groups. This is of interest since RNA polymerase is reduced with zinc deficiency in neonatal rat liver (27) and the RNA polymerase catalyzing the synthesis of ribosomal RNA in forebrain and whole brain were reduced in zinc deficiency (5). Apparently, the level of RNA is maintained in spite of reduced RNA polymerase activity.…”

Section: Discussionmentioning

confidence: 99%

Some Effects of Postnatal Zinc Deficiency on Developing Rat Brain

Al-Ubaidi

Sandstead

1975

Pediatric Research

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Extractpups suckled by zinc-adequate, pair-fed, or ad libitum-fed dams. The 12-day-old zincdeficient pups also displayed a A deficiency of dietary zinc during the suckling period of decreased incorporation of thymidine into D~~ and of the rat results in a depression in normal growth, which is, in inorganic sulfate into the acid-insoluble fraction of brain (19). part, due to the inanition experienced by the dam. The pups The studies reported in this paper are an extension of the from such zinc-deficient dams have smaller forebrains at all of above studies on effects of a deficiency of dietary zinc on the time intervals investigated in comparison with pups from brain composition and development during the suckling both zinc-adequate controls. The cell number of the forebrain period. of the zinc-deficient pup was also reduced in comparison with the zinc-adequate pups. RNA concentration did not appear to be affected, although the total RNA content was reduced METHODSbecause the smaller brain size. The protein per Pregnant Sprague-Dawley rats were fed an adequate ration cell was reduced at 6 and 15 days in the zinc-deficient ad libitum until delivery. soon as possible after parturition, forebrain and polysomal profiles displayed abnormal distribu-the dams were moved to clean Plexiglas cages. The number of tion of material between monosomes and polysomes in the in each litter was made equivalent at 8 and only litters zinc-deficient brain. Zinc deficiency during the suckling period that were maintained at or 8 pups during the test period thus appears to disadvantage the animal in terms of body, were used for analysis.animals were then fed a brain growth, accretion of cells into the forebrain, and normal biotin-enriched, 20% sprayed egg white diet (13) which was protein metabolism.modified to incorporate inositol at 1 g/kg and to omit the antibiotic. The zinc content was less than 1 mg/kg by analysis. Speculation One group of dams was given the diet ad libitum and distilled, zinc is essential for development of the brain. infants who deionized water to drink. Two zinc-adequate controls were experience zinc deficiency during the critical period for brain utilized. The first was pair-fed on an individual basis with the growth will suffer brain damage which may be poorly dams in the zinc-deficient group. The second control was fed reversible.the diet ad libitum. Both zinc-adequate controls received zinc at 100 mg/liter in their drinking water. At intervals, the entire litter of a dam was killed by Zinc has been known to be an essential trace element for decapitation. The brains were rapidly removed, separated by more than a century (17), although its essentiality for a blunt dissection into cerebellum and forebrain (the brain mammalian species was not established until 1934 (28), when anterior to the cerebellum excluding the brain stem and the deficiency syndrome was produced in rats. The manifesta-olfactory bulbs), and placed in ice-cold, buffered sucrose. tions of zinc deficiency have been reviewed by Underwood After wei...

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“…One possible explanation for this is that DNA polymerase and RNA polymerase were zinc metalloenzymes, [20][21][22] furthermore, Zn 2C played a very important role in cell differentiation and gene expression, 18,23 and therefore, changing the content of Zn 2C would probably influence transcription, translation and protein synthesis of E. coli P84A/MC1061. Furthermore, Zn 2C is an an activator of alcohol dehydrogenase and lactate dehydrogenase in the glycolysis process, 24,25 therefore, the glycolysis metabolic output of E. coli P84A/MC1061 would be promoted through the activating effect of Zn 2C absorbed from the broth, which maintained at a appropriate Zn 2C concentration pressure during lag phase and late log phase increased physiologic activity to a higher level resulting in a the higher metabolic output.…”

Section: Cmentioning

confidence: 99%

Effect of Zn²⁺ on halohydrin dehalogenase expression and accumulation through multi-parameter correlation research with Escherichia coli P84A/MC1061

Luo

Dong-rui

et al. 2017

Bioengineered

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2C was confirmed as the significant factor, and its optimal concentration for HheC expression was 3.87 mg/L through fermentation experiments in shaking flasks. Second, based on experimental results from the different strategies, it was found that PA, nutrient consumption rate (NCR) and specific growth rate (m) for E. coli P84A/MC1061 were promoted in the log phase (4-8 h) under appropriate Zn 2C concentrations in the lag phase and late log phase. Furthermore cell biomass was also increased to a higher level and the maximum HheC activity (i.e. HheC max ) was increased by 9.80%, and the time to reach HheC max was reduced from 16 to 12 hours. Furthermore, appropriate supplementation of Zn 2C caused higher m for E. coli P84A/MC1061, which resulted in more rapid accumulation of increased acetic acid concentrations, leading to higher acetic acid consumption avoiding any negative effects on producing HheC because of carbon source being exhausted prematurely and acetic acid being consumed rapidly.

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Decreased RNA Polymerase Activity in Mammalian Zinc Deficiency

Cited by 111 publications

References 15 publications

Nutriceutical potential of Pleurotus tuber-regium sclerotium

Nutriceutical potential of Pleurotus tuber-regium sclerotium

Some Effects of Postnatal Zinc Deficiency on Developing Rat Brain

Effect of Zn²⁺ on halohydrin dehalogenase expression and accumulation through multi-parameter correlation research with Escherichia coli P84A/MC1061

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Decreased RNA Polymerase Activity in Mammalian Zinc Deficiency

Cited by 111 publications

References 15 publications

Nutriceutical potential of Pleurotus tuber-regium sclerotium

Nutriceutical potential of Pleurotus tuber-regium sclerotium

Some Effects of Postnatal Zinc Deficiency on Developing Rat Brain

Effect of Zn2+ on halohydrin dehalogenase expression and accumulation through multi-parameter correlation research with Escherichia coli P84A/MC1061

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Effect of Zn²⁺ on halohydrin dehalogenase expression and accumulation through multi-parameter correlation research with Escherichia coli P84A/MC1061