Decreased oxygen uptake with stored blood in the isolated hindlimb

Yhap, E. O.; Wright, Creighton B.; Popovic, Nataljia; Alix, Eudéline

doi:10.1152/jappl.1975.38.5.882

Cited by 25 publications

(12 citation statements)

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“…However, unwanted changes, not directly related to the shift of the ODC, may be induced by the experimental setup per se. For instance, Yhap et al (34) have reported a decreased oxygen release in an isolated hind-limb preparation during perfusion with acid citrate dextrose (ACD)-stored blood compared with fresh ACD blood and ascribed the results to the leftward shift of the ODC. Ross and Hlastala (33) reproduced these experimental results with ACD blood, but when the ODC was shifted to the left by incubation of cells in sodium metabisulfite or sodium cyanate no such effect was seen.…”

Section: Discussionmentioning

confidence: 99%

Tissue oxygenation and muscular substrate turnover in two subjects with high hemoglobin oxygen affinity.

Wranne¹,

Berlin²,

Jorfeldt³

et al. 1983

J. Clin. Invest.

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A B S T R A C T Oxygen transport to and substrate turnover in leg muscle were studied at rest and during light and heavy upright bicycle exercise in two brothers with a hereditary hemoglobinopathy associated with high oxygen affinity (P50 = 13 mmHg). Femoral venous oxygen tension was below normal and femoral venous oxygen saturation above normal at rest and during exercise. Thus, the arterial-femoral venous oxygen saturation difference was decreased. Despite a compensatory increase in hemoglobin concentration, the arterial-femoral venous oxygen content difference tended to be below normal at heavy exercise. Approximately 25% of the oxygen was delivered via the abnormal hemoglobin at relative heavy exercise. Arterial lactate levels, lactate release, and muscle lactate concentration were not increased at any level of exercise. Glucose, alanine, pyruvate, and glycerol turnover were essentially normal, but the glycogen and creatine phosphate stores were abnormally depleted at the termination of heavy exercise. The exercise electrocardiogram (ECG) was normal, indicating that myocardial oxygenation was adequate. Muscle-surface oxygen pressure fields were normal at rest (not investigated during exercise). It is concluded that the high oxygen affinity of the hemoglobin in our two subjects did not lead to heart or skeletal muscle hypoxia during heavy exercise, as judged from the ECG and from the leg lactate turnover. Despite the lack of evidence for muscle hypoxia, the subjects experienced leg muscle fatigue and the creatine phosphate and glycogen stores were depleted more than normally.

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Section: Discussionmentioning

confidence: 99%

Tissue oxygenation and muscular substrate turnover in two subjects with high hemoglobin oxygen affinity.

Wranne¹,

Berlin²,

Jorfeldt³

et al. 1983

J. Clin. Invest.

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“…There has been mounting evidence which demonstrates that an elevated oxygen affinity of blood is disadvantageous in oxygen transport under normoxic state (VALTIS and KENNEDY, '1954;BUNN et al, 1969;YHAP et al, 1975;OCHIAI and ENOKI, 1976). Living organisms, however, respond to this dysfunction of blood in a variety of compensatory mechanisms, e.g.…”

Section: Discussionmentioning

confidence: 99%

Posttransfusional recovery of defective respiratory function of stored blood in dogs.

1986

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Changes in the oxygenation properties and the relevant biochemical parameters of canine blood were followed during its storage in acid-citrate-dextrose (ACD) medium (4°C) and after transfusion of the stored blood. Reductions of the blood P50 (pH 7.40, P~o2 40 Torr, 37°C) and 2,3-diphosphoglycerate (DPG) during storage was much slower than in human blood. Half decay time for DPG was 21 and 5 days for canine and human blood, respectively. The DPG decay could be extensively accelerated in the presence of bisulfite in ACD. The stored and DPGdepleted red cells required a considerably long period of time for the complete posttransfusional restoration of the DPG and P50. In five dogs whose blood were exchanged by 84% in average with the stored blood of mean DPG depletion of 88%, mean extents of the DPG and P50 restoration were 74 and 89% of the normal values after 24 h. After 5 days both the parameters were within the normal ranges. The red cell extraalong with intra-cellular pH fell immediately after the transfusion, and restored to the normal level after 24 h. From these results, correlations between P50 or red cell transmembrane pH gradient at the extracellular pH of 7.40 (dpH) and red cell DPG/hemoglobin molar ratio (x) were derived as P50 = 10.92x+ 14.2 (r=0.91) or dpH =0.060x+0. 135 (r=0.70).

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“…Lactate production is linear and hemolysis moderate. sion survival and cellular ATP levels [11,13,24,28], In vitro studies have demon strated that 2,3-DPG influences the prop erties of oxyhemoglobin [2,7], and in vivo studies have shown that the increased oxy gen affinity due to the depletion of this organic phosphate may have untoward ef fects [31,35], especially in patients with altered myocardial performance and coro nary artery disease who cannot compensate by increasing coronary blood flow and/or cardiac output [10,19]. The fall in pH is one of the principal causes of gradual dam age of the erythrocytes [4,18].…”

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confidence: 99%

Maintenance of 2,3-DPG and ATP Levels in Blood Stored for 30 Days at a Constant pH Obtained by Variation of C0(2)

Mendonca¹,

Appel²,

Bidault³

et al. 1978

Vox Sanguinis

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A novel procedure for storing blood at a controlled pH consists in collecting blood in a pH 8.20, Tris-CPD solution and storing it in a special recipient including a gas permeable membrane under a CO(2) atmosphere. The recipient is placed in an atmosphere of variable CO(2)content, so that the initial alkalinity of the preservative is balanced by dissolved CO(2), the proportion of which is diminished when lactate production increases with storage. 2,3-DPG and ATP were studied at three different pH levels of approximately 7.25, 7.45, and 7.65 at 4°C. The best pH for the simultaneous maintenance of 2,3-DPG and ATP was 7.65. Under these conditions, 2,3-DPG is maintained at its initial level and ATP at 55% of its initial level at the 30th day. Lactate production is linear and hemolysis moderate.

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Decreased oxygen uptake with stored blood in the isolated hindlimb

Cited by 25 publications

References 0 publications

Tissue oxygenation and muscular substrate turnover in two subjects with high hemoglobin oxygen affinity.

Tissue oxygenation and muscular substrate turnover in two subjects with high hemoglobin oxygen affinity.

Posttransfusional recovery of defective respiratory function of stored blood in dogs.

Maintenance of 2,3-DPG and ATP Levels in Blood Stored for 30 Days at a Constant pH Obtained by Variation of C0(2)

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