Decreased expression of response gene to complement 32 in psoriasis and its association with reduced M2 macrophage polarization

Kim, Hee Joo; Jang, Jae-Dong; Lee, Eun Hui; Jung, Sungwon; Roh, Joo Young; Jung, YunJae

doi:10.1111/1346-8138.14733

Cited by 10 publications

(10 citation statements)

References 7 publications

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“…Analysis of the marker genes for these clusters revealed biological subtypes including M2-like macrophages (37.3% of cells), M1 macrophages (35.7%), typical M2 macrophages (15.3%), dendritic cells (5.1%), foam macrophages (2.6%), neutrophils (2.5%), and two clusters of undefined cell types (1.4%). Our M1/M2 macrophages definition was based on previous published literature including [ 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 ] ( Table 2 ). We defined typical M2 by high expression of Trem2 and showed that they have expression patterns consistent with M2-like macrophages but with even higher expression of Slc9a3r2 , Timp2 , and Gpnmb ( Figure 3 b and Figure S1 ).…”

Section: Resultsmentioning

confidence: 99%

Single-Cell RNA-Sequencing Identifies Infrapatellar Fat Pad Macrophage Polarization in Acute Synovitis/Fat Pad Fibrosis and Cell Therapy

et al. 2021

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The pathogenesis and progression of knee inflammatory pathologies is modulated partly by residing macrophages in the infrapatellar fat pad (IFP), thus, macrophage polarization towards pro-inflammatory (M1) or anti-inflammatory (M2) phenotypes is important in joint disease pathologies. Alteration of M1/M2 balance contributes to the initiation and progression of joint inflammation and can be potentially altered with mesenchymal stem cell (MSC) therapy. In an acute synovial/IFP inflammation rat model a single intra-articular injection of IFP-MSC was performed, having as controls (1) diseased rats not receiving IFP-MSC and (2) non-diseased rats. After 4 days, cell specific transcriptional profiling via single-cell RNA-sequencing was performed on isolated IFP tissue from each group. Eight transcriptomically distinct cell populations were identified within the IFP across all three treatment groups with a noted difference in the proportion of myeloid cells across the groups. Largely myeloid cells consisted of macrophages (>90%); one M1 sub-cluster highly expressing pro-inflammatory markers and two M2 sub-clusters with one of them expressing higher levels of canonical M2 markers. Notably, the diseased samples (11.9%) had the lowest proportion of cells expressing M2 markers relative to healthy (14.8%) and MSC treated (19.4%) samples. These results suggest a phenotypic polarization of IFP macrophages towards the pro-inflammatory M1 phenotype in an acute model of inflammation, which are alleviated by IFP-MSC therapy inducing a switch towards an alternate M2 status. Understanding the IFP cellular heterogeneity and associated transcriptional programs may offer insights into novel therapeutic strategies for disabling joint disease pathologies.

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Section: Resultsmentioning

confidence: 99%

Single-Cell RNA-Sequencing Identifies Infrapatellar Fat Pad Macrophage Polarization in Acute Synovitis/Fat Pad Fibrosis and Cell Therapy

et al. 2021

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“…Response gene to complement (RGC)-32 is important for M2 macrophage polarization and phagocytic activity, and inhibits the development of M1 macrophages ( 108 ). The level of RGCC (the gene encoding RGC-32) mRNA was significantly lower in lesional psoriasis than in samples from normal individuals ( 95 ). Furthermore, Rgcc expression was significantly reduced in the lesional skin of imiquimod-induced psoriasiform dermatitis.…”

Section: Macrophagesmentioning

confidence: 99%

“…Furthermore, a considerable number of macrophages was observed in lesional skin ( 94 ). Immunofluorescence staining revealed that CD68 + iNOS + M1 macrophages were increased and CD68 + CD163 + M2 macrophages were decreased in human psoriasis lesional skin compared with skin samples from normal individuals ( 95 ). Another study demonstrated accumulation of dermal CD68 + macrophages that expressed TNF-α in human psoriatic plaques ( 96 ).…”

Section: Macrophagesmentioning

confidence: 99%

Dendritic Cells and Macrophages in the Pathogenesis of Psoriasis

Kamata

Tada

2022

Front. Immunol.

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Psoriasis is a chronic inflammatory skin disease characterized by scaly indurated erythema. This disease impairs patients’ quality of life enormously. Pathological findings demonstrate proliferation and abnormal differentiation of keratinocytes and massive infiltration of inflammatory immune cells. The pathogenesis of psoriasis is complicated. Among immune cells, dendritic cells play a pivotal role in the development of psoriasis in both the initiation and the maintenance phases. In addition, it has been indicated that macrophages contribute to the pathogenesis of psoriasis especially in the initiation phase, although studies on macrophages are limited. In this article, we review the roles of dendritic cells and macrophages in the pathogenesis of psoriasis.

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“…Based on above evidence and recent findings, considering the relationship of autophagy and psoriasis lesions [ 22 ], we propose the hypothesis that tattoos represent a “psoriasis-hostile” tissue environment pertained by a population of LAP active M2-polarized macrophages. Expanding our above observation of guttate psoriasis tending to spar tattoo areas, we assume that further study of the relationship of psoriasis lesions to tattooed skin may provide important insights into the role of macrophages in the pathogenesis of this disease.…”

Section: Discussion/conclusionmentioning

confidence: 67%

“…In human psoriasis, a subpopulation of classically activated proinflammatory macrophages plays a crucial role in the pathogenesis of skin lesions, and a preponderance of M1 macrophage activation state is also associated with increased PASI scores [ 21 ]. In accordance with above, markers of M2 macrophage polarization are reduced in psoriasis [ 22 ], and the improvement of psoriasis with TNF- α inhibitors correlates with the inhibition of the M1 macrophage polarization pathway [ 23 ]. Stimulated macrophages are the main immune-effector cell species in animal psoriasis models, like the inducible human TNF transgenic mouse line [ 24 ].…”

Section: Discussion/conclusionmentioning

confidence: 75%

A Guttate Psoriasis That Tends to Spare Three Tattoos: A Macrophage Liaison

Spyridonos

Zampeli

Rapti

et al. 2021

Case Reports in Dermatological Medicine

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Induction of new psoriasis sites was reported in only a small amount of psoriasis patients undergoing tattooing, despite the intuitive belief that tattoo trauma might awaken the disease due to the isomorphic phenomenon of Koebner. In this case report, we discuss a patient who presented with a remarkable sparing of his three tattoo sites during a guttate psoriasis flare-up that was unrelated to tattooing. The spatial concordance of tattoo and psoriasis lesions was analyzed on clinical pictures of tattoo sites taken during the psoriasis episode. For the quantification of the spatial distribution of the psoriasis lesions, Voronoi diagrams were generated, and coefficients of variation and the two-sample t-test were employed to compare the distributions of Voronoi patch sizes in different settings. Compared to skin areas without tattoos, a tattoo introduced a higher variation in the sizes of the Voronoi patches centered around psoriasis lesions. Based on our findings, we would like to discuss the possible role of macrophages as the key cellular link in the complex pathophysiologic relationship between tattooing/tattoo and psoriasis. Taking into account the relationship of autophagy and psoriasis lesions, we propose the hypothesis that tattoos represent a “psoriasis-hostile” tissue environment pertained by a population of LAP active M2-polarized macrophages. Further clinical studies of the relationship of psoriasis lesions to the tattooed skin are needed and may provide important insights into the role of macrophages in the pathogenesis of psoriasis.

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Decreased expression of response gene to complement 32 in psoriasis and its association with reduced M2 macrophage polarization

Cited by 10 publications

References 7 publications

Single-Cell RNA-Sequencing Identifies Infrapatellar Fat Pad Macrophage Polarization in Acute Synovitis/Fat Pad Fibrosis and Cell Therapy

Single-Cell RNA-Sequencing Identifies Infrapatellar Fat Pad Macrophage Polarization in Acute Synovitis/Fat Pad Fibrosis and Cell Therapy

Dendritic Cells and Macrophages in the Pathogenesis of Psoriasis

A Guttate Psoriasis That Tends to Spare Three Tattoos: A Macrophage Liaison

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