Dendritic Cells and Macrophages in the Pathogenesis of Psoriasis

Kamata, Masahiro; Tada, Yayoi

doi:10.3389/fimmu.2022.941071

Cited by 73 publications

(45 citation statements)

References 131 publications

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“… 9 Some studies have found that macrophages contribute to the pathogenesis of psoriasis, especially in the initiation stage. 10 In addition, T cells and neutrophils play an important role in the occurrence and development of psoriasis. 11 , 12 Thus, psoriasis and immunity are inseparable and it is necessary to reveal the immune cell spectrum of psoriasis mice.…”

Section: Introductionmentioning

confidence: 99%

Single-Cell RNA Sequencing Reveals the Immune Cell Profiling in IMQ Induced Psoriasis-Like Model

Jin¹,

Wang²,

Qie³

et al. 2022

JIR

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Purpose Psoriasis is a chronic systemic inflammatory skin disease with a high recurrence rate. The immune response plays an important role in psoriasis. However, the subsets of immune cells involved in inflammation in psoriatic mice have not been fully studied. This study showed the immune environment characteristics of psoriasis in mice. Methods We used single-cell RNA sequencing (10× Genomics) as an unbiased analytical strategy to investigate the heterogeneity of skin immune cells in imiquimod-induced psoriasis mice systematically. Results We identified 10 major clusters and their marker genes among 14,439 cells. The proportions of macrophages, NK/T cells, conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs) were increased in psoriatic mice. Macrophages were the largest group and were further divided into 7 subgroups, and all macrophage clusters were increased in psoriatic mice. Differentially expressed genes in control versus psoriatic mice skin lesions showed that Fcgr4, Saa3 and Acp5 in macrophages, Acp5, Fcgr4 and Ms4a6d in NK/T cells, Saa3 in cDCs, and Ifitm1 in pDCs were upregulated in psoriasis mice. Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis emphasized the role of oxidative phosphorylation signals and antigen processing and presentation signals in murine psoriasis-like models. Conclusion Our study reveals the immune environment characteristics of the commonly used IMQ induced psoriasis-like models and provides a systematic insight into the immune response of mice with psoriasis, which is conducive to comparing the similarities and differences between the mouse model and human psoriasis.

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Section: Introductionmentioning

confidence: 99%

Single-Cell RNA Sequencing Reveals the Immune Cell Profiling in IMQ Induced Psoriasis-Like Model

Jin¹,

Wang²,

Qie³

et al. 2022

JIR

View full text Add to dashboard Cite

show abstract

“…Furthermore, the infiltration of M1-Mø is another key characteristic of psoriasis. Previous studies have shown that the infiltration of M1-Mø greatly elevates the expression of inflammatory cytokines such as interleukins and TNF-α, which triggers psoriasis by stimulating the proliferation and inflammation of keratinocytes (Kamata and Tada, 2022). To date, little is known about the regulatory effects of ELE on Mø.…”

Section: Discussionmentioning

confidence: 99%

“…Elevated expression of M1-Mø is another key characteristic of psoriasis, it has been found that various inflammatory cytokines such as ILs and TNF-α secreted by M1-Mø contribute to psoriasis by triggering inflammation in keratinocytes (Kamata and Tada, 2022). We therefore stimulated Mø using LPS/INF-γ to obtain M1-Mø in vitro and then examined the effects of ELE on the gene expression of key inflammatory cytokines in M1-Mø.…”

Section: Ele Indirectly Attenuates Keratinocytes Inflammation By Medi...mentioning

confidence: 99%

Dissolvable hyaluronic acid microneedles loaded with β-Elemene for the treatment of psoriasis

et al. 2022

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The pathology of psoriasis involves the over-proliferation of keratinocytes, exaggerated inflammation of keratinocytes, and infiltration of inflammatory cells such as macrophages (Mø), etc. The therapeutic outcomes of current treatment targeting one single pathological process are less than satisfactory. Based on their diverse biological activities, natural products offer a potential solution to this problem. In this study, we investigated the effects of β-Elemene (ELE) on both psoriatic keratinocytes and M1-type Mø (M1-Mø) in vitro. Hyaluronic acid (HA) microneedles loaded with ELE (HA-ELE-MN) were also fabricated and tested for the treatment of psoriasis in vivo using an imiquimod (IMQ)-induced psoriatic mice model. Our data suggest that ELE induces apoptosis and inhibits inflammation of psoriatic keratinocytes. In addition, ELE attenuates the expression of inflammatory cytokines secreted from M1-Mø, thus indirectly inhibiting the inflammation of keratinocytes. Furthermore, HA-ELE-MN has been found to significantly alleviate symptoms in an IMQ-induced psoriatic mice model by inducing keratinocytes apoptosis, suppressing keratinocytes proliferation, and inhibiting M1-Mø infiltration. Taken together, this study demonstrates that ELE can be used for the treatment of psoriasis by targeting both keratinocytes and M1-Mø, which provides a potential novel reagent for psoriasis treatment.

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“…Although psoriasis is predominately a T-cell mediated disease, infiltration of monocytes/macrophages has been well characterized ( Vestergaard et al, 2004 ; Kamata and Tada 2022 ). A significant increase in 15-HETE was detected in monocytes from patients with psoriatic arthritis ( Wójcik et al, 2019 ).…”

Section: Alox15b In Inflammation and Diseasementioning

confidence: 99%

Arachidonate 15-lipoxygenase type B: Regulation, function, and its role in pathophysiology

2022

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As a lipoxygenase (LOX), arachidonate 15-lipoxygenase type B (ALOX15B) peroxidizes polyenoic fatty acids (PUFAs) including arachidonic acid (AA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and linoleic acid (LA) to their corresponding fatty acid hydroperoxides. Distinctive to ALOX15B, fatty acid oxygenation occurs with positional specificity, catalyzed by the non-heme iron containing active site, and in addition to free PUFAs, membrane-esterified fatty acids serve as substrates for ALOX15B. Like other LOX enzymes, ALOX15B is linked to the formation of specialized pro-resolving lipid mediators (SPMs), and altered expression is apparent in various inflammatory diseases such as asthma, psoriasis, and atherosclerosis. In primary human macrophages, ALOX15B expression is associated with cellular cholesterol homeostasis and is induced by hypoxia. Like in inflammation, the role of ALOX15B in cancer is inconclusive. In prostate and breast carcinomas, ALOX15B is attributed a tumor-suppressive role, whereas in colorectal cancer, ALOX15B expression is associated with a poorer prognosis. As the biological function of ALOX15B remains an open question, this review aims to provide a comprehensive overview of the current state of research related to ALOX15B.

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Dendritic Cells and Macrophages in the Pathogenesis of Psoriasis

Cited by 73 publications

References 131 publications

Single-Cell RNA Sequencing Reveals the Immune Cell Profiling in IMQ Induced Psoriasis-Like Model

Single-Cell RNA Sequencing Reveals the Immune Cell Profiling in IMQ Induced Psoriasis-Like Model

Dissolvable hyaluronic acid microneedles loaded with β-Elemene for the treatment of psoriasis

Arachidonate 15-lipoxygenase type B: Regulation, function, and its role in pathophysiology

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