Decreased expression of MUC2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients

Wibowo, Agung Ary; Pardjianto, Bambang; Sumitro, Sutiman Bambang; Kania, Nia; Handono, Kusworini

doi:10.1016/j.bbrep.2019.100655

Cited by 14 publications

(11 citation statements)

References 25 publications

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“…The examination of colon specimens from UC patients indicated that MUC2 expression significantly decreased compared to that from healthy individuals [72] . The mucus layer was also revealed to become thinner and even absent in some parts, independent of local inflammation [ 73 , 74 ], and the inner mucus layer of patients with active UC is reported to be more penetrable [ 74 , 75 ].…”

Section: Detection Of Muc2 Mucus Barrier Dysfunction Intestinal Bacterial Invasion and Dysbiosis During Uc Development Clinically And Manmentioning

confidence: 98%

MUC2 and related bacterial factors: Therapeutic targets for ulcerative colitis

et al. 2021

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The mucin2 (MUC2) mucus barrier acts as the first barrier that prevents direct contact between intestinal bacteria and colonic epithelial cells. Bacterial factors related to the MUC2 mucus barrier play important roles in the response to changes in dietary patterns, MUC2 mucus barrier dysfunction, contact stimulation with colonic epithelial cells, and mucosal and submucosal inflammation during the occurrence and development of ulcerative colitis (UC). In this review, these underlying mechanisms are summarized and updated, and related interventions for treating UC, such as dietary adjustment, exogenous repair of the mucus barrier, microbiota transplantation and targeted elimination of pathogenic bacteria, are suggested. Such interventions are likely to induce and maintain a long and stable remission period and reduce or even avoid the recurrence of UC. A better mechanistic understanding of the MUC2 mucus barrier and its related bacterial factors may help researchers and clinicians to develop novel approaches for treating UC.

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Section: Detection Of Muc2 Mucus Barrier Dysfunction Intestinal Bacterial Invasion and Dysbiosis During Uc Development Clinically And Manmentioning

confidence: 98%

MUC2 and related bacterial factors: Therapeutic targets for ulcerative colitis

et al. 2021

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“…Muc2 degradation of LS174T cell surface by Amuc_1434* was detected by immunofluorescence staining [31,57]. LS174T cells (10 6 cells/mL) were cultured on 6-well tissue culture plates at 37 • C overnight.…”

Section: Muc2 Immunofluorescencementioning

confidence: 99%

A Purified Aspartic Protease from Akkermansia Muciniphila Plays an Important Role in Degrading Muc2

Meng

Wang

Lan

et al. 2019

IJMS

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Akkermansia muciniphila can produce various mucin-degrading proteins. However, the functional characteristics of these proteins and their role in mucin degradation are unclear. Of the predicted protein-coding genes, Amuc_1434, which encodes for a hypothetical protein, is the focus in this study. A recombinant enzyme Amuc_1434 containing the 6× His-tag produced in Escherichia coli (hereinafter termed Amuc_1434*) was isolated to homogeneity and biochemically characterised. Results showed that the enzyme can hydrolyse hemoglobin with an activity of 17.21 U/μg. The optimal pH and temperature for hemoglobin hydrolysis of Amuc_1434* were found to be around 8.0 and 40 °C, respectively. Amuc_1434* is identified as a member of the aspartic protease family through the action of inhibitor pepstatin A. Amuc_1434* promotes the adhesion of colon cancer cell line LS174T, which can highly express Muc2. Significantly Amuc_1434* can degrade Muc2 of colon cancer cells. Amuc_1434 is mainly located in the colon of BALB/c mice. These results suggest that the presence of Amuc_1434 from Akkermansia muciniphila may be correlated with the restoration of gut barrier function by decreasing mucus layer thickness.

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“…Mucins are glycoproteins present in mucus, and gel‐forming mucins, namely, muc5ac‐ like and muc5d ( muc2‐ like), were significantly altered in zebrafish fed soya bean. During intestinal inflammation in humans, muc2 was downregulated because of apoptotic defects (Wibowo et al., 2019). An upregulation of muc5ac in the intestine cells of humans by food‐derived peptides has been reported previously (Martínez‐Maqueda et al., 2012).…”

Section: Discussionmentioning

confidence: 99%

Zebrafish intestinal transcriptome highlights subdued inflammatory responses to dietary soya bean and efficacy of yeast β‐glucan

Rehman

Gora

Siriyappagouder

et al. 2021

Journal of Fish Diseases

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The intestinal epithelium acts as a selective barrier, and the epithelial cells evoke appropriate responses to microbial signals and antigenic factors (Soderholm & Pedicord, 2019). Normal functioning of the associated mucosal barrier, which includes the epithelial layer and lamina propria, is vital to carry out digestion and nutrient absorption by the intestine (Farré et al., 2020). A compromised mucosal barrier increases the permeability of the intestine, and such a condition allows undesirable luminal antigens to cross the barrier, leading to dysbiosis and activation of inflammatory response in the tissue. Damage to intestinal tissue can eventually result in the disturbance of the overall gut physiology (Farré et al., 2020).Certain dietary components can alleviate issues related to intestinal disorders, whereas some others trigger intestinal inflammation (Hou et al., 2014;Khoshbin & Camilleri, 2020). Specific dietary components like soya saponins, the anti-nutritional factor in soya bean meal, can bind to the intestinal epithelial membrane cholesterol.

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Decreased expression of MUC2 due to a decrease in the expression of lectins and apoptotic defects in colitis patients

Cited by 14 publications

References 25 publications

MUC2 and related bacterial factors: Therapeutic targets for ulcerative colitis

MUC2 and related bacterial factors: Therapeutic targets for ulcerative colitis

A Purified Aspartic Protease from Akkermansia Muciniphila Plays an Important Role in Degrading Muc2

Zebrafish intestinal transcriptome highlights subdued inflammatory responses to dietary soya bean and efficacy of yeast β‐glucan

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