Decreased Erythrocyte NA⁺,K⁺-ATPase Activity and Increased Plasma TBARS in Prehypertensive Patients

Abstract

The essential hypertension has been associated with membrane cell damage. The aim of the present study is investigate the relationship between erythrocyte Na+,K+-ATPase and lipoperoxidation in prehypertensive patients compared to normotensive status. The present study involved the prehypertensive patients (systolic: 136 ± 7 mmHg; diastolic: 86.8 ± 6.3 mmHg; n = 8) and healthy men with normal blood pressure (systolic: 110 ± 6.4 mmHg; diastolic: 76.1 ± 4.2 mmHg; n = 8) who were matched for age (35 ± 4 years old). The venous blood samples of antecubital vein (5 mL) were collected into a tube containing sodium heparin as anticoagulant (1000 UI), and erythrocyte ghosts were prepared for quantifying Na+,K+-ATPase activity. The extent of the thiobarbituric acid reactive substances (TBARS) was determined in plasma. The statistical analysis was carried out by Student's t-test and Pearson's correlation coefficient. A P < 0.05 was considered significant. The Na+,K+-ATPase activity was lower in prehypertensive patients compared with normotensive subjects (4.9 versus 8.0 nmol Pi/mg protein/min; P < 0.05). The Na+,K+-ATPase activity correlated negatively with TBARS content (r = −0.6; P < 0.05) and diastolic blood pressure (r = −0.84; P < 0.05). The present study suggests that Na+,K+-ATPase activity reduction and elevation of the TBARS content may underlie the pathophysiological aspects linked to the prehypertensive status.

Anthocyanins suppress the secretion of proinflammatory mediators and oxidative stress, and restore ion pump activities in demyelination

“…Evidence in the literature has demonstrated a decrease in both the activity and the expression of Na + ,K + -ATPase and Ca 2+ -ATPase in the development of neurodegenerative diseases, as well as in MS, due to axonal degeneration and a reduction in the synaptic terminals [15][16][17]. Moreover, the marked reduction of these ions pumps can occur due to the oxidation of the SH groups present in its structure, as well as the oxidation of the lipid membranes that lead to a destabilization of the optimal conformation and ion transport [102,56,[103][104][105][106]. Additionally, we found a correlation between the decreases in Na + ,K + -ATPase and Ca 2+ -ATPase activity and the increase in markers of oxidative stress (Fig.…”

Protective Effects of Ferulic Acid on High Glucose-Induced Protein Glycation, Lipid Peroxidation, and Membrane Ion Pump Activity in Human Erythrocytes

“…The erythrocyte membranes were prepared according to a previous method with minor modification [ 22 ]. Erythrocyte samples were lysed with Tris-HCl (15 mM, pH 7.4) and centrifuged at 12,000 g at 4°C for 30 min.…”

Fluoride Exposure Induces Inhibition of Sodium-and Potassium-Activated Adenosine Triphosphatase (Na+, K+-ATPase) Enzyme Activity: Molecular Mechanisms and Implications for Public Health

“…Past studies have also shown that reduced NKA activity may underlie the pathophysiological aspects linked to the prehypertensive status in humans [40,545]. Additionally, it has been widely documented that inhibition of NKA activity is associated with hypertension [44,45,46,47,48,49,50,51,52,53].…”