Decreased ecto-5′-nucleotidase activity in rabbit lymphocytes during experimental lensinduced uveitis

Uusitalo, Hannu; Uusitalo, Risto; Palkama, Arto

doi:10.3109/02713688408997227

Cited by 3 publications

(1 citation statement)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This has been taken to mean that the decrease in enzyme activity is due to immunological disease and not dependent on the inflammation in the eye. This is in agreement with our results in experimental animals where immunization with heterologous lens protein induced a decrease in lymphocyte AMPase activity (Uusitalo et al 1983).…”

Section: Discussionsupporting

confidence: 94%

Ecto 5‘‐nucleotidase Activity in Lymphocytes in Cases of Uveitis

Uusitalo

Palkama

1983

Acta Ophthalmologica

Self Cite

View full text Add to dashboard Cite

This study attempts to demonstrate cellular reactivity in cases of uveitis. Lymphocyte AMPase activity was measured in 59 patients with uveitis of different types, all in the active stage. The patients were divided into 3 groups: 1) anterior uveitis with known disease, 2) anterior uveitis with unknown aetiology, and 3) posterior uveitis. The AMPase activity in peripheral lymphocytes from uveitis patients with known disease (group 1) was significantly decreased in comparison to lymphocytes from controls. Patients having endogenous uveitis (unknown aetiology, group 2) showed heterogeneity concerning their enzyme activity, and no significant changes were demonstrated. A subpopulation of patients with very low AMPase activity in the active stage of their disease could be defined. Patients with posterior uveitis showed no change in their lymphocyte AMPase activity. B-cells were also counted in cell suspension to verify that there was no selective change in the B-cells. Cell suspensions from patients with low and normal AMPase activities were studied by the electron microscope and found to have similar proportions of different types of mononuclear cells. Enzyme deficiencies in lymphocytes were associated with immunological disorders and would reflect dysfunction of lymphocytes. The heterogenous results from patients with endogenous uveitis were interpreted as showing cellular reactivity to ocular damage caused by a variety of mechanisms.

show abstract

Section: Discussionsupporting

confidence: 94%

Ecto 5‘‐nucleotidase Activity in Lymphocytes in Cases of Uveitis

Uusitalo

Palkama

1983

Acta Ophthalmologica

Self Cite

View full text Add to dashboard Cite

show abstract

Anterior uveitis in children

Uusitalo

Mahlberg

1991

Graefe's Arch Clin Exp Ophthalmol

View full text Add to dashboard Cite

In this prospective study of childhood uveitis, 75 children with anterior uveitis were analyzed to determine whether a relationship could be found between the occurrence of uveitis, its clinical features, and humoral immunity to retinal S-antigen. For the purposes of analysis, children were divided into acute (18 cases) and chronic (57 cases) categories, depending on the duration of ocular inflammation. Clinical features of both of these groups were compared and significant differences were found in the occurrence of unilateral vs bilateral involvement, the incidence of complications, and visual outcome. Serum samples from children with acute (7 cases) and chronic uveitis (28 cases) and from healthy children (132 cases) were tested for antibodies to S-antigen by enzyme-linked immunosorbent assay (ELISA). A statistically significant difference in the level of specific antibodies between patients with chronic uveitis and controls was found. However, there was no difference between children with acute uveitis and healthy patients, nor was there any correlation between the severity of uveitis and antibody titer. For further elucidation of the significance of circulating anti-S antibodies, 14 children with chronic anterior uveitis were followed for as long as 18 months after the initial visit. Multiple serum antibody titers to bovine retinal S-antigen were determined and compared with the clinical activity at the time of each sampling. In only 6 of 14 patients did the titers to S-antigen tend to decrease with clinical improvement and stabilize at titers somewhat higher than normal values.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract