Decreased Activity and Expression of Intestinal Oligopeptide Transporter PEPT1 in Rats with Hyperthyroidism in Vivo

Ashida, Kayoko; Katsura, Toshiya; Saya, Hideyuki; Inui, Ken Ichi

doi:10.1023/b:pham.0000029285.45101.ee

Cited by 19 publications

(10 citation statements)

References 33 publications

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“…3C). As we know, K m value represents the transport function of transporter, while V max value means the expression of transporter (Ashida et al, 2004;Miao et al, 2011). In other words, the reduction of V max value for dioscin indicated the expression of Oatps might be decreased in ANIT-induced cholestasis.…”

Section: Discussionmentioning

confidence: 99%

Dioscin protects against ANIT–induced cholestasis via regulating Oatps, Mrp2 and Bsep expression in rats

Zhang

Jia

et al. 2016

Toxicology and Applied Pharmacology

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Section: Discussionmentioning

confidence: 99%

Dioscin protects against ANIT–induced cholestasis via regulating Oatps, Mrp2 and Bsep expression in rats

Zhang

Jia

et al. 2016

Toxicology and Applied Pharmacology

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“…Eight-week-old male Wistar rats were housed in a temperature-and humidity-controlled room and fed rat chow ad libitum. Hyperthyroidism was induced by adding T 4 (12 mg/l) to the drinking water for 21 days as previously described (Ashida et al, 2004). After treatment, the kidney, liver, jejunum, and ileum were excised.…”

Section: Methodsmentioning

confidence: 99%

Modulation of P-Glycoprotein Expression in Hyperthyroid Rat Tissues

Nishio¹,

Katsura²,

Ashida³

et al. 2005

Drug Metab Dispos

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ABSTRACT:P-glycoprotein (Pgp) is expressed in various normal tissues and plays an important role in drug absorption and disposition. In addition, it is supposed that alterations in the expression levels of Pgp are involved in the inter-and intraindividual variability of pharmacokinetics of many drugs. Since pharmacokinetic properties of various drugs are altered in patients with thyroid disorders, we examined the expression of Pgp and mdr1a/1b mRNA in the kidney, liver, jejunum, and ileum from euthyroid and hyperthyroid rats. Western blot analysis revealed that Pgp expression was markedly increased in the kidney and liver of hyperthyroid rats. In contrast, it was slightly increased in the jejunum and ileum. mdr1a/1b mRNA levels were significantly increased in the kidney of hyperthyroid rats. However, they were not increased in the liver as well as in the jejunum and ileum of hyperthyroid rats. Expression levels of bile salt export pump and mdr2 mRNA were also unchanged in hyperthyroid rat liver. Taken together, these findings suggest that thyroid hormone induces Pgp expression in a tissue-selective manner, and that the modulation of mdr1a/1b mRNA expression in the hyperthyroid state varies among tissues.P-glycoprotein (Pgp) is expressed in various tissues such as brain, liver, kidney, and intestine (Cordon-Cardo et al., 1990;Brady et al., 2002) and plays an important role in defining the pharmacokinetics of many drugs. Pgp functions as a drug efflux pump and exports hydrophobic, bulky drugs such as anticancer agents, cardiac glycosides, ␤-blockers, calcium channel blockers, and immunosuppressants. Since Pgp has a broad substrate recognition, the concomitant administration of drugs often causes drug interactions by inhibiting Pgpmediated transport (Yu, 1999). For example, inhibition of digoxin transport in cultured epithelial cell lines expressing Pgp by various drugs such as quinidine (Tanigawara et al., 1992;Fromm et al., 1999), verapamil (Tanigawara et al., 1992, and cyclosporin A (Okamura et al., 1993) has been reported. We have also demonstrated that the renal clearance of digoxin was decreased in patients receiving a concomitant administration of clarithromycin and, accordingly, the plasma concentration of digoxin was increased (Wakasugi et al., 1998). The mechanism of this interaction was explained by the inhibition of Pgp-mediated tubular secretion of digoxin. On the other hand, recent studies have demonstrated that changes in the expression levels of Pgp affect the pharmacokinetic properties of Pgp substrates. Greiner et al. (1999) reported that rifampin administration induced Pgp expression in the small intestine and reduced the plasma concentration of orally administered digoxin, suggesting that alterations in the expression levels of Pgp are closely involved in the inter-and intraindividual variability of pharmacokinetics of Pgp substrates.Thyroid hormone is secreted from the thyroid gland to maintain normal growth and development, normal body temperature, and normal energy levels. Most of its effect...

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“…On the one hand, Rosebrough, Russell, and Richards () reported that high dietary protein increases T4 and Insulin‐like growth factor 1, while decreases plasma triiodothyronine and growth hormone. On the other hand, Ashida, Katsura, Saito, and Inui () induced hyperthyroidism by T4 supplementing of drinking water for 21 days in rats, resulting in a decrease in expression of intestinal PepT1 mRNA. Conversely, Lu and Klaassen () induced hypothyroidism by thyroidectomy in rats, resulted in increased PepT1 expression in the kidney.…”

Section: Discussionmentioning

confidence: 99%

Impact of mash and crumble diets on intestinal amino acids transporters, intestinal morphology and pancreatic enzyme activity of broilers

Mahdavi

Osmanyan

Фисинин

et al. 2018

Animal Physiology Nutrition

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The objective of this study was to evaluate effects of mash and crumble pre-starter diets on pancreatic enzyme activity, intestinal morphology, gene expression of intestinal peptide and amino acid (AA) transporters of broilers. Broilers in battery cages were assigned to different feed forms of pre-starter diet from 1 to 10 days of age. Significantly increased body weight gain (BWG), feed intake (FI) and lowered FCR were observed in birds fed crumble pre-starter diet (CPD, p < 0.05). Feed forms had no effect on whole and small intestine length, but relative intestinal length and relative small intestinal length significantly increased in the broilers fed a mash pre-starter diet (MPD, p < 0.05). Feeding CPD increased the weight of pancreas (p < 0.05), but relative weight of the pancreas was not influenced by treatments. Pancreatic protease and amylase activities significantly increased in the broilers fed CPD (p < 0.05) but the activity of lipase was not influenced. Crypt depth (CD) and villus height (VH) were higher in broilers fed CPD (p < 0.05) but villus width (VW), villus surface area (VSA) and villus height-to-crypt depth ratio (VCR) were not influenced by treatments. mRNA levels for peptide transporter 1 (PepT1), Na -independent cationic AA transporter1 (CAT1), Na -independent cationic and Na -dependent neutral AA transporter 1 (y LAT1) and Na -dependent neutral AA transporter (B AT) were lower in birds fed CPD (p < 0.05). There were no differences in mRNA abundance of Na -independent cationic and zwitterionic AA transporter (b AT) among treatments. Overall, the present data showed that feeding crumble diet during first 10 days of age, through higher FI, enhanced intestinal histomorphology, increased digestive enzyme activity is beneficial to growth performance of broilers. Indeed, dietary form can be an important factor in the expression of jejunal transporters.

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Decreased Activity and Expression of Intestinal Oligopeptide Transporter PEPT1 in Rats with Hyperthyroidism in Vivo

Abstract: These results indicate that in hyperthyroid rats, the activity and expression of PEPT1 were decreased in the small intestine.

Cited by 19 publications

References 33 publications

Dioscin protects against ANIT–induced cholestasis via regulating Oatps, Mrp2 and Bsep expression in rats

Dioscin protects against ANIT–induced cholestasis via regulating Oatps, Mrp2 and Bsep expression in rats

Modulation of P-Glycoprotein Expression in Hyperthyroid Rat Tissues

Impact of mash and crumble diets on intestinal amino acids transporters, intestinal morphology and pancreatic enzyme activity of broilers

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