Decreased abundance of <i><scp>F</scp>aecalibacterium prausnitzii</i> in the gut microbiota of <scp>C</scp>rohn's disease

Fujimoto, Takehide; Imaeda, Hirotsugu; Takahashi, Kenichiro; Kasumi, Eiji; Bamba, Shigeki; Fujiyama, Yoshihide; Andoh, Akira

doi:10.1111/jgh.12073

Cited by 250 publications

(179 citation statements)

References 47 publications

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“…14,34 The relative decrease of present and active F. prausnitzii (mean decrease from 2.42% to 0.46% at the DNA level and from 1.00% to 0.10% at the RNA level) as a consequence of AB usage is particularly crucial: apart from the above-mentioned anti-inflammatory capacity, 33 this species is known to be less abundant in individuals suffering from inflammatory bowel diseases like Crohn's disease (CD) or UC. [35][36][37] A statistically significant association of prior AB usage and CD was reported as well. 38 Taking this into account, we argue that AB-induced reduction of intestinal species such as F. prausnitzii could support the onset of intestinal disorders, possibly fostering CD or UC.…”

Section: Ab Therapy Leads To a Reduction Of Beneficial Otusmentioning

confidence: 92%

Dynamic changes of the luminal and mucosa-associated gut microbiota during and after antibiotic therapy with paromomycin

Heinsen

Knecht

Neulinger³

et al. 2015

Gut Microbes

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(2015) Dynamic changes of the luminal and mucosa-associated gut microbiota during and after antibiotic therapy with paromomycin, Gut Microbes, 6:4, 243-254, DOI: 10.1080/19490976.2015 To link to this article: https://doi.org/10. 1080/19490976.2015 Keywords: 16S rRNA gene, antibiotics, lumen, microbiota, mucosaGut microbiota play a key role in the host's health system. Broad antibiotic therapy is known to disrupt the microbial balance affecting pathogenic as well as host-associated microbes. The aim of the present study was to investigate the influence of antibiotic paromomycin on the luminal and mucosa-associated microbiota at the DNA (abundance) and RNA (potential activity) level as well as to identify possible differences. The influence of antibiotic treatment on intestinal microbiota was investigated in 5 healthy individuals (age range: 20-22 years). All participants received the antibiotic paromomycin for 3 d. Fecal samples as well as sigmoidal biopsies were collected before and immediately after cessation of antibiotic treatment as well as after a recovery phase of 42 d. Compartment-and treatment statusspecific indicator operational taxonomic units (OTUs) as well as abundance-and activity-specific patterns were identified by 16S rRNA and 16S rRNA gene amplicon libraries and high-throughput pyrosequencing. Microbial composition of lumen and mucosa were significantly different at the DNA compared to the RNA level. Antibiotic treatment resulted in changes of the microbiota, affecting the luminal and mucosal bacteria in a similar way. Several OTUs were identified as compartment-and/or treatment status-specific. Abundance and activity patterns of some indicator OTUs differed considerably. The study shows fundamental changes in composition of gut microbiota under antibiotic therapy at both the potential activity and the abundance level at different treatment status. It may help to understand the complex processes of gut microbiota changes involved in resilience mechanisms and on development of antibiotic-associated clinical diseases.

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Section: Ab Therapy Leads To a Reduction Of Beneficial Otusmentioning

confidence: 92%

Dynamic changes of the luminal and mucosa-associated gut microbiota during and after antibiotic therapy with paromomycin

Heinsen

Knecht

Neulinger³

et al. 2015

Gut Microbes

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“…These clostridia induced IL-10-producing T reg cells that attenuated colitis in mice [9]. Faecalibacterium prausnitzii belongs to Clostridium cluster IV, and its abundance is significantly decreased in Crohn's disease patients as compared with healthy individuals [91]. In addition, oral administration of either live Faecalibacterium prausnitzii or its supernatant markedly reduced the severity of TNBS-induced colitis through the induction of IL-10 [17].…”

Section: ? T Cells or Transfer Of Foxp3mentioning

confidence: 96%

A breakthrough in probiotics: Clostridium butyricum regulates gut homeostasis and anti-inflammatory response in inflammatory bowel disease

2015

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Intestinal immune homeostasis is regulated by gut microbiota, including beneficial and pathogenic microorganisms. Imbalance in gut bacterial constituents provokes host proinflammatory responses causing diseases such as inflammatory bowel disease (IBD). The development of next-generation sequencing technology allows the identification of microbiota alterations in IBD. Several studies have shown reduced diversity in the gut microbiota of patients with IBD. Advances in gnotobiotic technology have made possible analysis of the role of specific bacterial strains in immune cells in the intestine. Using these techniques, we have shown that Clostridium butyricum as a probiotic induces interleukin-10-producing macrophages in inflamed mucosa via the Toll-like receptor 2/myeloid differentiation primary response gene 88 pathway to prevent acute experimental colitis. In this review, we focus on the new approaches for the role of specific bacterial strains in immunological responses, as well as the potential of bacterial therapy for IBD treatments.

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“…have been associated with the production of butyrate necessary for the health of colonic epithelial tissue [10,11], and have been shown to be depleted in inflammatory bowel disease [12]. Unusual polysaccharide binding and degradation strategies have been described in Ruminococcus flavefaciens [13,14], while another Ruminococcaceae, Faecalibacterium prausnitzii, has been shown to be depleted in Crohn's disease [15].…”

Section: Lachnospiraceae and Ruminococcaceae Are Active Members Of Thmentioning

confidence: 99%

Untangling the Genetic Basis of Fibrolytic Specialization by Lachnospiraceae and Ruminococcaceae in Diverse Gut Communities

et al. 2013

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Abstract:The Lachnospiraceae and Ruminococcaceae are two of the most abundant families from the order Clostridiales found in the mammalian gut environment, and have been associated with the maintenance of gut health. While they are both diverse groups, they share a common role as active plant degraders. By comparing the genomes of the Lachnospiraceae and Ruminococcaceae with the Clostridiaceae, a more commonly free-living group, we identify key carbohydrate-active enzymes, sugar transport mechanisms, and metabolic pathways that distinguish these two commensal groups as specialists for the degradation of complex plant material.

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Decreased abundance of Faecalibacterium prausnitzii in the gut microbiota of Crohn's disease

Cited by 250 publications

References 47 publications

Dynamic changes of the luminal and mucosa-associated gut microbiota during and after antibiotic therapy with paromomycin

Dynamic changes of the luminal and mucosa-associated gut microbiota during and after antibiotic therapy with paromomycin

A breakthrough in probiotics: Clostridium butyricum regulates gut homeostasis and anti-inflammatory response in inflammatory bowel disease

Untangling the Genetic Basis of Fibrolytic Specialization by Lachnospiraceae and Ruminococcaceae in Diverse Gut Communities

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