Decrease of insoluble glucan formation in Streptococcus mutans by co-cultivation with Enterococcus faecium T7 and glucanase addition

Abstract

ObjectivesTo develop preventive canine oral health bio-materials consisting of probiotics and glucanase to reduce insoluble glucan and volatile sulfur compound formation.ResultsCo-cultivation of Enterococcus faecium T7 with Streptococcus mutans at inoculation ratio of 3:1 (v/v) resulted in 25% reduction in the growth of Streptococcus mutans. Amounts of soluble and insoluble glucans produced by S. mutans were decreased to 70 and 55%, respectively. Insoluble glucan was decreased from 0.6 µg/ml in S. mutans culture to 0.03 µg/ml in S. mutans co-cultivated with E. faecium T7 in the presence of Lipomyces starkeyi glucanase. Volatile sulfur compound, a main component of halitosis produced by Fusobacteria nucleatum, was decreased by co-cultivating F. nucleatum with E. faecium. Conclusion E. faecium and glucanase can be combined as potentially active ingredients of oral care products for pets by reducing plaque-forming bacteria growth and their by-products that cause cavity and periodontal disease.Electronic supplementary materialThe online version of this article (10.1007/s10529-017-2478-z) contains supplementary material, which is available to authorized users.

Oral probiotic activities and biosafety of Lactobacillus gasseri HHuMIN D

“…In one study, S. mutans created artificial dental plaque was suppressed by 53% by E. faecium T7 co-culture [ 29 ]. In another experiment, Lactobacillus lactis 1370 suppressed plaque formation by 95% [ 67 ].…”

“…To assess whether and the degree to which L. gasseri HHuMIN D prevents the development of artificial dental plaque by S. mutans , beaker wire tests following the protocol of Yu et al [ 29 ] were conducted. Five percent sucrose was added to 40 ml of broth consisting of equivalent quantities of BHI and MRS broth and the pH was adjusted to 7.0 by the addition of 0.1 M MOPS buffer.…”

Oral Probiotic Activities and Biosafety of Lactobacillus gasseri HHuMIN D

“…To validate the degree to which L. gasseri HHuMIN D prevents the development of arti cial dental plaque by S. mutans, beaker wire tests following the protocol of Yu, et al [33] were conducted. Five percent sucrose was added to 40 ml of broth consisting of equivalent quantities of BHI and MRS broth and the pH was adjusted to 6.9 to 7.0 by the addition of 0.1 M MOPS buffer.…”

Anti-cariogenic Characteristics of Rubusoside

“…The pellet was dissolved in 1 M NaOH. The amount of waterinsoluble glucan was measured via thin-layer chromatography (TLC) [12]. One μl of sample was spotted onto a precoated TLC plate (Merck, Darmstadt, Germany) and developed with two ascents of acetonitrile:water (85:15, v/v).…”

“…Carbohydrates were visualized by dipping the TLC plate into 0.5% (w/v) N-(1-naphtyl) ethylenediamine dihydrochloride (Sigma Aldrich, USA) and 5% (w/v) sulfuric acid (Duksan Chemicals, South Korea) in methanol (Duksan Chemicals, South Korea) followed by heating at 120°C for 5 min. The amount of carbohydrates was analyzed by conversion to the integrated density value using the AlphaEaseFC 4.0 Image Program (Alpha Inotech, CA, USA) [12].…”