Deconstruction of lignocelluloses: potential biological approaches

Sahay, Sanjay

doi:10.1016/b978-0-12-822810-4.00010-5

Cited by 4 publications

(3 citation statements)

References 185 publications

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“…The fundamental task of (hemi)cellulolytic enzymes, active on linear polysaccharides chains, is greatly improved by biocatalysts able to break down the chemical linkages ( Figure 3 ) which strictly interconnect the different plant cell wall polymers. These enzymes, commonly named auxiliary enzymes, have a relevant role in substituting traditional chemical pretreatments, aiding in fermentable sugar recovery and allowing the extraction of powerful bioactive compounds, mainly of polyphenolic nature, that crosslink plant cell wall components [ 47 ]. Their biotechnological relevance makes their expression, through the transplastomic platform, of particular interest in a circular economy perspective.…”

Section: New Challenges For Biocatalysts and The Circular Economymentioning

confidence: 99%

Plastid Transformation: New Challenges in the Circular Economy Era

Tamburino

Marcolongo²,

Sannino

et al. 2022

IJMS

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In a circular economy era the transition towards renewable and sustainable materials is very urgent. The development of bio-based solutions, that can ensure technological circularity in many priority areas (e.g., agriculture, biotechnology, ecology, green industry, etc.), is very strategic. The agricultural and fishing industry wastes represent important feedstocks that require the development of sustainable and environmentally-friendly industrial processes to produce and recover biofuels, chemicals and bioactive molecules. In this context, the replacement, in industrial processes, of chemicals with enzyme-based catalysts assures great benefits to humans and the environment. In this review, we describe the potentiality of the plastid transformation technology as a sustainable and cheap platform for the production of recombinant industrial enzymes, summarize the current knowledge on the technology, and display examples of cellulolytic enzymes already produced. Further, we illustrate several types of bacterial auxiliary and chitinases/chitin deacetylases enzymes with high biotechnological value that could be manufactured by plastid transformation.

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Section: New Challenges For Biocatalysts and The Circular Economymentioning

confidence: 99%

Plastid Transformation: New Challenges in the Circular Economy Era

Tamburino

Marcolongo²,

Sannino

et al. 2022

IJMS

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“…In terrestrial environments, Lignocellulose is the main product of photosynthesis and represents on average the most abundant renewable plant biomass bio-resource in the world [3]. Lignocellulosic material is mainly composed of three structural polymers, namely cellulose, hemicellulose, and lignin, and is one of the best options as an ecological raw material of industrial and biotechnological interest, as it is both inexpensive and easily available [4,5]. Their valorization reduces the use of fossil fuels and preserves the natural environment [6].…”

Section: Introductionmentioning

confidence: 99%

Screening of Cellulolytic Bacteria from Various Ecosystems and Their Cellulases Production under Multi-Stress Conditions

et al. 2022

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Cellulose represents the most abundant component of plant biomass on earth; it is degraded by cellulases, specific enzymes produced by microorganisms. However, cellulases of bacterial origin attract more interest due to their natural diversity and ability to inhabit a variety of niches, allowing the selection of cellulolytic strains resistant to environmental stresses. The screening of the cellulolytic activity of 398 bacteria isolated from various ecosystems in Algeria (cave, ruins, chott, thermal station, and rhizosphere of arid and semi-arid regions) was performed by the appearance of a hydrolysis zone on carboxymethylcellulose (CMC) medium. The cellulase activity on CMC (1%) broth allowed to select 26 strains among which 12 had the best activity (0.3 U/mL to 2.2 U/mL). Optimization of physicochemical parameters (salinity: 0–1 M NaCl; pH: 3, 4, 7, 9, and 11; temperature: 30, 45, and 50 °C; PEG8000: 0 and 30%) involved in growth and cellulose production showed that the majority of strains were mesophilic, neutrophilic, or alkali- tolerant and tolerant to 30% of PEG8000. The cellulase activity and stability under different stress allowed to retain five strains, which the most efficient. Based on the 16S-rRNA sequencing results, they belonged to the genus Bacillus. The physicochemical properties of cellulases (crude extract) showed a CMCase active over a wide range of pH (4 to 11), optimal at 50 °C and 60 °C. The inhibiting salinity effect on the activity was not detected and was negligible on the enzymatic stability. The residual CMCase activity remained between 40 and 70% in a temperature range between 40 and 70 °C, was stable over a wide range of saline concentrations (0–2000 mM), and was weakly affected at 30% of PEG8000. The crude enzyme extract was able to hydrolyze both soluble and insoluble cellulosic substrates. The evaluation of the hydrolysis capacity of lignocellulosic waste revealed the ability of tested strains to degrade wheat bran, barley bran, and corncob. In addition, the enzyme showed significant multi-stress resistance on solid and liquid media. By these characteristics, these cellulolytic bacteria could be attractive to be used in various industrial and biotechnology applications.

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“…Durante a degradação de materiais lignocelulósicos, esses microrganismos produzem uma ampla gama de enzimas hidrolíticas, incluindo celulase, xilanases, proteases, lipases, fosfatases e lactase, que levam à degradação da parede celular vegetal, e consequentemente, perda de resistência mecânica. 24,58 Por muito tempo essas limitações impediram a exploração do bambu como fonte de matéria-prima para o desenvolvimento de novos materiais com aplicações comerciais.…”

Section: Exploração Da Matriz Lignocelulósica Do Bambu Para Novos Mat...unclassified

Material De Carbono Condutor a Partir Da Pirólise Do Bambu Para Aplicações Elétricas, Eletrotérmicas E Eletroquímicas

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Deconstruction of lignocelluloses: potential biological approaches

Cited by 4 publications

References 185 publications

Plastid Transformation: New Challenges in the Circular Economy Era

Plastid Transformation: New Challenges in the Circular Economy Era

Screening of Cellulolytic Bacteria from Various Ecosystems and Their Cellulases Production under Multi-Stress Conditions

Material De Carbono Condutor a Partir Da Pirólise Do Bambu Para Aplicações Elétricas, Eletrotérmicas E Eletroquímicas

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