Plastid Transformation: New Challenges in the Circular Economy Era

Tamburino, Rachele; Marcolongo, Loredana; Sannino, Lorenza; Ionata, Elena; Scotti, Nunzia

doi:10.3390/ijms232315254

Cited by 3 publications

(3 citation statements)

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“…In plant breeding, cp genomes can serve as a valuable source of genetic markers, which can be employed for the development of improved crop varieties with desirable traits, such as increased yield, resistance to pests and diseases, and enhanced stress tolerance [ 28 ]. In biotechnology, cp genomes offer opportunities for the engineering of transplastomic plants, which can produce high levels of bioactive compounds, biopharmaceuticals, or functional proteins for industrial, pharmaceutical, or agricultural applications [ 29 ]. Lastly, the comprehensive knowledge of cp genome diversity and evolution can aid in the conservation of plant biodiversity by providing essential information for the identification of endangered species, the assessment of genetic diversity within and among populations, and the formulation of effective conservation strategies [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis and characterization of the chloroplast genome of Krascheninnikovia ceratoides (Amarathaceae): a xerophytic semi-shrub exhibiting drought resistance and high-quality traits

Liu,

Zheng,

et al. 2024

BMC Genom Data

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Background Krascheninnikovia ceratoides, a perennial halophytic semi-shrub belonging to the genus Krascheninnikovia (Amarathaceae), possesses noteworthy ecological, nutritional, and economic relevance. This species is primarily distributed across arid, semi-arid, and saline-alkaline regions of the Eurasian continent, encompassing Inner Mongolia, Xinjiang, Qinghai, Gansu, Ningxia, and Tibet. Results We reported the comprehensive chloroplast (cp) genome of K. ceratoides, characterized by a circular conformation spanning 151,968 bp with a GC content of 36.60%. The cp genome encompassed a large single copy (LSC, 84,029 bp), a small single copy (SSC, 19,043 bp), and a pair of inverted repeats (IRs) regions (24,448 bp each). This genome harbored 128 genes and encompassed 150 simple sequence repeats (SSRs). Through comparative analyses involving cp genomes from other Cyclolobeae (Amarathaceae) taxa, we observed that the K. ceratoides cp genome exhibited high conservation, with minor divergence events in protein-coding genes (PCGs) accD, matK, ndhF, ndhK, ycf1, and ycf2. Phylogenetic reconstructions delineated K. ceratoides as the sister taxon to Atriplex, Chenopodium, Dysphania, and Suaeda, thus constituting a robust clade. Intriguingly, nucleotide substitution ratios (Ka/Ks) between K. ceratoides and Dysphania species for ycf1 and ycf2 genes surpassed 1.0, indicating the presence of positive selection pressure on these loci. Conclusions The findings of this study augment the genomic repository for the Amarathaceae family and furnish crucial molecular instruments for subsequent investigations into the ecological adaptation mechanisms of K. ceratoides within desert ecosystems.

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Section: Introductionmentioning

confidence: 99%

Comparative analysis and characterization of the chloroplast genome of Krascheninnikovia ceratoides (Amarathaceae): a xerophytic semi-shrub exhibiting drought resistance and high-quality traits

Liu,

Zheng,

et al. 2024

BMC Genom Data

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“…capitata ), cauliflower ( Brassica oleracea var. botrytis ), sugar beet ( Beta vulgaris ), eggplant ( Solanum melongena ), carrot ( Daucus carota ), cotton ( Gossypium hirsutum ), and silver poplar ( Populus alba ) have been produced [ 154 , 155 ]. Quite recently, major success was achieved in the production of fertile transplastomic Arabidopsis thaliana plants [ 156 ].…”

Section: Specific Features Of the Organization Of The Plastid Transcr...mentioning

confidence: 99%

Three Parts of the Plant Genome: On the Way to Success in the Production of Recombinant Proteins

Розов

Zagorskaya

Konstantinov

et al. 2022

Plants

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Recombinant proteins are the most important product of current industrial biotechnology. They are indispensable in medicine (for diagnostics and treatment), food and chemical industries, and research. Plant cells combine advantages of the eukaryotic protein production system with simplicity and efficacy of the bacterial one. The use of plants for the production of recombinant proteins is an economically important and promising area that has emerged as an alternative to traditional approaches. This review discusses advantages of plant systems for the expression of recombinant proteins using nuclear, plastid, and mitochondrial genomes. Possibilities, problems, and prospects of modifications of the three parts of the genome in light of obtaining producer plants are examined. Examples of successful use of the nuclear expression platform for production of various biopharmaceuticals, veterinary drugs, and technologically important proteins are described, as are examples of a high yield of recombinant proteins upon modification of the chloroplast genome. Potential utility of plant mitochondria as an expression system for the production of recombinant proteins and its advantages over the nucleus and chloroplasts are substantiated. Although these opportunities have not yet been exploited, potential utility of plant mitochondria as an expression system for the production of recombinant proteins and its advantages over the nucleus and chloroplasts are substantiated.

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“…To develop this circular network, the ability of microorganisms to convert raw substrates into small molecules and proteins is exploited via fermentation processes, using either wild-type or engineered organisms ( Cherubini, 2010 ). However, many interesting feedstocks, such as lignocellulosic biomass, usually require enzymatic pre-treatment to become bioaccessible for the microorganism ( Tamburino et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free PDH promoter

Bernat-Camps

Ebner²,

Schusterbauer³

et al. 2023

Front. Bioeng. Biotechnol.

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The current transition towards the circular bioeconomy requires a rational development of biorefineries to sustainably fulfill the present demands. The use of Komagataella phaffii (Pichia pastoris) can meet this challenge, since it has the capability to use crude glycerol as a carbon-source, a by-product from the biodiesel industry, while producing high- and low-added value products. Recombinant protein production (RPP) using K. phaffii has often been driven either by the methanol induced AOX1 promoter (PAOX1) and/or the constitutive GAP promoter (PGAP). In the last years, strong efforts have been focused on developing novel expression systems that expand the toolbox variety of K. phaffii to efficiently produce diverse proteins that requires different strategies. In this work, a study was conducted towards the development of methanol-free expression system based on a heat-shock gene promoter (PDH) using glycerol as sole carbon source. Using this promoter, the recombinant expression is strongly induced in carbon-starving conditions. The classical PGAP was used as a benchmark, taking for both strains the lipase B from Candida antarctica (CalB) as model protein. Titer of CalB expressed under PDH outperformed PGAP controlled expression in shake-flask cultivations when using a slow-release continuous feeding technology, confirming that PDH is induced under pseudo-starving conditions. This increase was also confirmed in fed-batch cultivations. Several optimization rounds were carried out for PDH under different feeding and osmolarity conditions. In all of them the PDH controlled process outperformed the PGAP one in regard to CalB titer. The best PDH approach reached 3.6-fold more specific productivity than PGAP fed-batch at low μ. Compared to the optimum approach for PGAP-based process, the best PDH fed-batch strategy resulted in 2.3-fold higher titer, while the specific productivity was very similar. To summarize, PDH is an inducible promoter that exhibited a non-coupled growth regulation showing high performance, which provides a methanol-free additional solution to the usual growth-coupled systems for RPP. Thus, this novel system emerges as a potential alternative for K. phaffii RPP bioprocess and for revaluing crude glycerol, promoting the transition towards a circular economy.

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Plastid Transformation: New Challenges in the Circular Economy Era

Cited by 3 publications

References 131 publications

Comparative analysis and characterization of the chloroplast genome of Krascheninnikovia ceratoides (Amarathaceae): a xerophytic semi-shrub exhibiting drought resistance and high-quality traits

Comparative analysis and characterization of the chloroplast genome of Krascheninnikovia ceratoides (Amarathaceae): a xerophytic semi-shrub exhibiting drought resistance and high-quality traits

Three Parts of the Plant Genome: On the Way to Success in the Production of Recombinant Proteins

Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free PDH promoter

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