Decomposition of phosphatidylethanolamine with phospholipase C from Clostridium perfringens

Dyatlovitskaya, É. V.; Volkova, V. I.; Ispolatovskaya, M. V.; Bergelson, L.D.

doi:10.1007/bf00912457

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…However, the acyl composition given above differs from that of egg phosphatidylcholine reported by other workers (e.g. Dyatlovitskaya, Volkova & Bergel'son, 1967) Area/molecule (A2) 120 Fig. 1.…”

Section: Resultscontrasting

confidence: 59%

The interaction of fetuin with phosphatidylcholine monolayers. Characterization of a lipoprotein membrane system suitable for the attachment of myxoviruses

Tiffany

Blough

1970

Biochemical Journal

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1. An artificial membrane system was formed by spreading at air/water and oil/water interfaces, by using phosphatidylcholine and the glycoprotein fetuin (mol.wt. 48400). 2. The plot of increase of interfacial pressure against amount of protein added beneath a monomolecular film of phosphatidylcholine showed two discontinuities, corresponding to the completion of two distinct layers of protein: (a) largely denatured and closely associated with the polar head groups of phosphatidylcholine, possibly with penetration of non-polar protein groups between the phosphatidylcholine molecules and (b) an additional adsorbed layer of substantially native fetuin in either a close-packed or open-lattice array. A more compactly organized membrane was apparently formed at pH7.4 with 1mm-Mg(2+) in the aqueous phase than without Mg(2+); at 15mm-Mg(2+), more random adsorption of protein appeared to take place. Qualitatively similar results were obtained at pH5.1 with 1mm-Mg(2+). Closer initial packing of the phosphatidylcholine layer decreased both the magnitude of the interfacial pressure change and the amounts of protein bound in the two layers. 3. The amount of N-acetylneuraminic acid released by neuraminidase (EC 3.2.1.18) in the subphase was measured at pH5.1; a mean distribution of 9.7x10(13) residues/cm(2) was calculated for the completed second protein layer.

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Section: Resultscontrasting

confidence: 59%

The interaction of fetuin with phosphatidylcholine monolayers. Characterization of a lipoprotein membrane system suitable for the attachment of myxoviruses

Tiffany

Blough

1970

Biochemical Journal

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“…Indeed, an eukaryotic cells have phospholipase C that cleave link between glycerine and the phosphate group in most types of phospholipids, however phospholipase C of an eukaryotesdoes not disconnect this link in PtdE [47,48]. The enzyme, cleaving this link precisely in PtdE, was found only in pathogenic bacteria Clostridium perfringens, synthesizing «aggressive enzymes» such as proteinase, lecithinase, collagenase, hyaluronidase and α-toxin, which is a phospholipase C [49].In eukaryotic cells such enzyme is still not detected for PtdE, so the reliability of the first assumptions is unlikely. It can be presumed that the accumulation of the PEA is due to a breach of PtdE synthesis at the stage of PEA and CTP interaction with the participation of CTP: phosphoethanolamine cytidylyltransferase.…”

Section: Pеа and Taurine In The Brain Of Vertebrates At Low Temperaturesmentioning

confidence: 99%

Influence of Low Temperature on the Evolution of Amino Acid Pools Adaptive Modifications inPoikilothermal Animals

Каранова¹

2013

ijbb

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Interpretation of phylogenesis in the light of comparative biochemistry is currently still limited. Adaptive changes in the concentration of the FAA in response to the decrease in temperature are one of the essential features of poikilothermic animals living in areas with large temperature difference. It is purpose of this review to reveal the hierarchy of certain biochemical sign, namely the FAA that underlie of differences in invertebrate and vertebrate poikilotherms and in the process of evolution begin to discover themselves only at low temperatures. The review summarizes literature and author information about evolutionary development of adaptive FAA responses of poikilothermic animals, at different levels of phylogenesis, as a result of the seasonal drop in temperature to negative or zero values. It is concluded that the non-specific accumulation of proteinogenic amino acids, characteristic for many invertebrates, is replaced in the vertebrates animals by the accumulation of new participants in the mechanisms of the low temperatures adaptation, which is a nonproteinogenic sulphoamino acid, taurine, and in the brain it is РЕА and phosphoamine acid, РS.

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Decomposition of phosphatidylethanolamine with phospholipase C from Clostridium perfringens

Cited by 2 publications

References 14 publications

The interaction of fetuin with phosphatidylcholine monolayers. Characterization of a lipoprotein membrane system suitable for the attachment of myxoviruses

The interaction of fetuin with phosphatidylcholine monolayers. Characterization of a lipoprotein membrane system suitable for the attachment of myxoviruses

Influence of Low Temperature on the Evolution of Amino Acid Pools Adaptive Modifications inPoikilothermal Animals

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