Decomposition of oxalic acid and other organic acids by streptomycetes as a taxonomic aid

Nitsch, B.; Kutzner, H. J.

doi:10.1002/jobm.19690090804

Cited by 3 publications

(2 citation statements)

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“…These tests were also used in the probability matrices of Langham et al (1989a), who found that the utilization of melibiose was an additional differentiating character. The utilization of organic acids such as malonate for identification purposes was recommended by Nitsch & Kutzner (1969).…”

Section: P Kampfer and R M Kroppenstedtmentioning

confidence: 99%

Probabilistic identification of streptomycetes using miniaturized physiological tests

Kämpfer

Kroppenstedt

1991

Journal of General Microbiology

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A frequency matrix of positive results for phena defined in a previous phenetic classification was constructed. A total of 329 physiological characters from 782 strains was taken as the basis for this identification matrix. The minimum number of diagnostic characters for the matrix was selected using computer programs for calculation of different separation indices (CHARSEP) and selection of group diagnostic properties (DIACHAR). The resulting matrix consisted of 52 phena versus 50 characters. Overlap of phena was found to be relatively small (OVERMAT program). Identification scores for the most typical hypothetical organism of each phenon was satisfactory (MOSTTYP program). The matrix was evaluated theoretically and practically (MATIDEN program). For members of major clusters and subclusters, e.g. Streptomyces albidoflavus, S. halstedii, S. griseus, S. fradiae, S. rimosus and S. albus, identification scores were high. Organisms of phena containing only small numbers of strains could be identified correctly, but with lower accuracy. The identification rate of the matrix (Willcox probability > 0.90) in the theoretical evaluation was 84.39%, and in the practical evaluation 78.12%.

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Section: P Kampfer and R M Kroppenstedtmentioning

confidence: 99%

Probabilistic identification of streptomycetes using miniaturized physiological tests

Kämpfer

Kroppenstedt

1991

Journal of General Microbiology

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“…Although it is generally considered that amylase and protease are mainly fungal and eubacterial products, the results of starch hydrolysis and protease expression. The production of citrase, urease, catalase and oxidase are considered for characterizing Streptomyces (Nitsch and Kutzner, 1969;Gotoh et al, 1982). The hydrogen sulphide and melanin production has been considered as the other important characters for the identification of actinomycetes (Shirling and Gottlieb, 1966)…”

Section: Indole Testmentioning

confidence: 99%

Biochemical studies on antibiotic production from Streptomyces griseus

2023

JPTCP

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The present study was aimed for identify Streptomyces griseus by biochemical tests. All Streptomyces spp. isolates (MH1, MH7, MH3, MH17, MH25) were screened for their antibacterial activity using scross-streak technique against Gram-negative (Pseudomonas aeruginosa and Escherichia coli) and Gram-positive (Staphylococcus aureus). Screening was performed by agar-well diffusion method and growth inhibition zones were measured in millimeters for each of the Streptomyces spp. isolates MH1, MH7, MH3, MH25. Tested isolates have shown potent in vitro antibacterial activities against all tested pathogens. The highest activities were shown by isolate MH7 against Staphylococcus aureus (21 mm), Pseudomonas aeruginosa (17.8 mm), Escherichia coli (22.5 mm). Streptomyces spp isolates (MH1, MH7, MH3, MH25) were selected for biochemical studies. All isolates hydrolyzed starch. All isolates produced Catalase, Gelatinase, Protease, Urease, Amylase, Cellulase, Chitinase and Lipase. All Streptomyces spp. isolates have ability to reduce nitrate, Tyrosine degradation and Casein hydrolysis. Hydrogen sulphide (H2S) production, Oxidase production, Indole production and Melanine reaction were studied for Streptomyces spp. isolates(MH1, MH7, MH3, MH25). Most of the isolates were not H2S producer, Oxidase, Indole and Melanine reaction except one isolate (MH3). All Streptomyces spp. isolates could Citrate utilization and Pectin degradation except MH7. Streptomyces spp. isolates 4 isolate utilizes eight carbon sources (Carbohydrates utilization) such as the Glucose, Galactose, Fructose, Sucrose, Xylose, Maltose, Lactose and Mannitol). Results of Streptomyces spp. isolates by Utilization of nitrogen (amino acid) sources (L-arginine ,L-isoleucine, Lcysteine, L-glycine, L-tyrosine and L-alanine). Four Streptomyces spp. isolates were selected, characterized based and identified by biochemical examination according to Bergey's Manual of determinative bacteriology. The bacteria was described to genus Streptomyces and species griseus. Given 4 isolates are presumptively the same or similar species (e.g., Streptomyces griseus). All isolates to have possessed similar biochemical tests results as the ones that were Streptomyces griseus.

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