Biochemical studies on antibiotic production from Streptomyces griseus

Abstract

The present study was aimed for identify Streptomyces griseus by biochemical tests. All Streptomyces spp. isolates (MH1, MH7, MH3, MH17, MH25) were screened for their antibacterial activity using scross-streak technique against Gram-negative (Pseudomonas aeruginosa and Escherichia coli) and Gram-positive (Staphylococcus aureus). Screening was performed by agar-well diffusion method and growth inhibition zones were measured in millimeters for each of the Streptomyces spp. isolates MH1, MH7, MH3, MH25. Tested isolates have shown potent in vitro antibacterial activities against all tested pathogens. The highest activities were shown by isolate MH7 against Staphylococcus aureus (21 mm), Pseudomonas aeruginosa (17.8 mm), Escherichia coli (22.5 mm). Streptomyces spp isolates (MH1, MH7, MH3, MH25) were selected for biochemical studies. All isolates hydrolyzed starch. All isolates produced Catalase, Gelatinase, Protease, Urease, Amylase, Cellulase, Chitinase and Lipase. All Streptomyces spp. isolates have ability to reduce nitrate, Tyrosine degradation and Casein hydrolysis. Hydrogen sulphide (H2S) production, Oxidase production, Indole production and Melanine reaction were studied for Streptomyces spp. isolates(MH1, MH7, MH3, MH25). Most of the isolates were not H2S producer, Oxidase, Indole and Melanine reaction except one isolate (MH3). All Streptomyces spp. isolates could Citrate utilization and Pectin degradation except MH7. Streptomyces spp. isolates 4 isolate utilizes eight carbon sources (Carbohydrates utilization) such as the Glucose, Galactose, Fructose, Sucrose, Xylose, Maltose, Lactose and Mannitol). Results of Streptomyces spp. isolates by Utilization of nitrogen (amino acid) sources (L-arginine ,L-isoleucine, Lcysteine, L-glycine, L-tyrosine and L-alanine). Four Streptomyces spp. isolates were selected, characterized based and identified by biochemical examination according to Bergey's Manual of determinative bacteriology. The bacteria was described to genus Streptomyces and species griseus. Given 4 isolates are presumptively the same or similar species (e.g., Streptomyces griseus). All isolates to have possessed similar biochemical tests results as the ones that were Streptomyces griseus.

Diversity of Actinomycetes Isolated from Peat Soil of Undistrubed Forest and Pineapple Plantation in Sessang, Sarawak

“…It was also noted in this study that Streptomyces griseus strain PS42 can be considered the most potent producer of enzymes screened (Table 3). This statement was supported by Talib Saleh et al (2023), where the researchers observed that S. griseus has the ability to produce multiple enzymes such as celllulase, lipase and protease.…”

Cellulolytic potentials of aspergillus oryzae and streptomyces griseus isolated from waste dump soil in Nile University Of Nigeria, Abuja

“…Arora et al (2005), had reported that Streptomyces griseus B1 isolated from soil, when grown on cellulose powder as submerged culture produced high levels of all the three components of the cellulolytic enzyme systems. Also, in the research by Al-Rubaye et al (2023), different strains of S. griseus were able to utilize cellulose in variable degrees. The ability of S. griseus to utilize cellulose even much more than Aspergillus www.scienceworldjournal.org ISSN: 1597-6343 (Online), ISSN: 2756 Published by Faculty of Science, Kaduna State University oryzae, which belongs to a family of well-established cellulose utilizers as reported in the present study, could be lying in the report of Sato et al (2020) which asserts that the genome contains six lytic polysaccharide monooxygenases (LPMO)10-encoding genes that phylogenetically cluster with cellulose or chitin targeting LPMO10s.…”