Decolourization of reactive black 5 by laccase: Optimization by response surface methodology

Murugesan, Kumarasamy; Dhamija, Ankur; Nam, In-Hyun; Kim, Young Mo; Chang, Yoon Seok

doi:10.1016/j.dyepig.2006.04.020

Cited by 155 publications

(89 citation statements)

References 38 publications

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“…The crude laccase from solid-state culture of T. trogii SYBC-LZ and G. lucidum could decolorize RB 5 only in the presence of a mediator (Murugesan et al, 2007b;Zeng et al, 2011). Murugesan et al (2007a) reported the same observation about the dye decolorization activity of purified laccase of P. sajor caju. Camarero et al (2005) also reported that RB 5 and Azure B could be decolorized only in the presence of mediators.…”

Section: Discussionsupporting

confidence: 63%

Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii

Yeşilada¹,

Birhanlı²,

Ercan³

et al. 2014

Turk J Biol

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The effect of various factors on dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii ATCC 200800 to obtain rapid and high decolorization activity against Reactive Black 5 and Reactive Blue 171 dyes was investigated. All conditions used were important for dye decolorizing activity of this crude laccase enzyme. The optimum pH of decolorization was tested at 30 °C and it was around 3.0. This activity was highly reduced at pH 4.5-6.0. On the other hand, the optimum temperature for rapid and high decolorization was 50 °C. Importantly, the decolorization rate of crude laccase at pH 4.5 and pH 6.0 increased with the rise in temperature from 30 °C to 50 °C. Therefore, high decolorization could be obtained at pH 4.5 and even pH 6.0 by selecting the proper temperature for these pH values. Enzyme amount also affected the dye decolorization positively. This crude laccase could also decolorize the mixed dyes (Reactive Black 5 and Reactive Blue 171) and synthetic wastewaters. Native polyacrylamide gel electrophoresis showed the responsibility of the laccase enzyme in decolorization. Rapid and high textile dye decolorization through the selection of appropriate conditions could facilitate the development of more economical and environmentally friendly processes.

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Section: Discussionsupporting

confidence: 63%

Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii

Yeşilada¹,

Birhanlı²,

Ercan³

et al. 2014

Turk J Biol

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“…Laccase from Ascomycete mauginiella was found to be optimum at 40°C (Palonen et al, 2003) and 30°C for B. subtilis WD23 laccase (Wang et al, 2013) which was consistent with current results. Moreover, the laccase produced from B. subtilis MTCC 2414 was found to be active at pH 7, which indicates that the results is well accordance with laccase produced from R. praticola (Murugesan et al, 2007).…”

Section: Discussionsupporting

confidence: 72%

“…Initially, M S 66.2 kDa 45 kDa ~ 37 kDa the dyes were pre-heated with 40% sulfuric acid at a constant concentration of 0.01% (w/v). The final volume of culture filtrates (5 mL; 2.5×10 6 CFU mLG 1 ), free enzymes (50 U) and immobilized laccase (100 U) were analyzed and experiments were carried out under shaking conditions (150 rpm) at 40°C for different time intervals from 0-120 h. The residual dye concentration was monitored spectrophotomertrically (Hitachi U-2800 double beam spectrophotometer) at the maximum visible wavelength of each dye (Orange 3R-493 nm, T-Blue-664 nm, Yellow GR-430 nm) (Murugesan et al, 2007). Dye degradation was expressed in terms of percentage.…”

Section: Effect Of Ph and Thermal Stabilitymentioning

confidence: 99%

Application of Immobilized Laccase from Bacillus subtilis MTCC 2414 on Decolourization of Synthetic Dyes

Narayanan¹,

Sevanan²,

Eva³

et al. 2015

Research J. of Microbiology

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Laccases (benzenediol oxygen oxidoreductases, EC 1.10.3.2) are polyphenol oxidases (PPO) that catalyze the oxidation of various substituted phenolic compounds by using molecular oxygen as the electron acceptor. In the present study, Bacillus subtilis MTCC 2414 were employed for the production of laccase using guaiacol as a substrate under Submerged Fermentation (SmF) condition. Laccase was partially purified by salt precipitation method followed by dialysis. The synthetic dyes such as Orange 3R, Yellow GR and T-Blue were used for degradation studies using culture filtrate, free and immobilized laccases. The immobilized laccase obtained by entrapment method using sodium alginate was optimized and it exhibited maximum activity at pH 7 (321 U mLG 1 ) and temperature 35°C (317 U mLG 1 ). Similarly, free laccase was also optimized and the maximum activity was observed at pH 9 (309 U mLG 1 ) and temperature 35°C (339 U mLG 1 ). Surprisingly, Yellow GR dye was found to be highly degraded up to 81.72% by immobilized laccase when compared to free laccase (74.69% of T-Blue) and culture filtrate (72.16% of Orange 3R) respectively. The FTIR spectrum showed a spectrum peaks at 2110.12 c/m in control dye and degraded Yellow GR exposed peak at 3406.36 cmG 1 which represents C-N stretching of aromatic amine group in dyes. Therefore, high degradation ability of laccase from B. subtilis MTCC 2414 makes them attractive catalyst for biotechnological applications.

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“…Hydrogen ions concentration (pH) strongly impacts the enzymatic reactions and is receptive to hydrogen ion concentration present in the medium across the cellular membrane (Murugesan et al, 2007). Most laccase activity turned into determined at pH five for Streptomyces lavendulae, after a period of 3 hrs (Fig.…”

Section: Resultsmentioning

confidence: 99%

Production and Optimization of Laccase from Streptomyces lavendulae

Mishra¹,

Srivastava²,

Prakash³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Decolourization of reactive black 5 by laccase: Optimization by response surface methodology

Cited by 155 publications

References 38 publications

Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii

Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii

Application of Immobilized Laccase from Bacillus subtilis MTCC 2414 on Decolourization of Synthetic Dyes

Production and Optimization of Laccase from Streptomyces lavendulae

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